PMID:2477358: Difference between revisions

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<span style = 'font-size: large'> '''Claverie-Martin, F, Diaz-Torres, MR, Yancey, SD and Kushner, SR'''  (1989) Cloning of the altered mRNA stability (ams) gene of Escherichia coli K-12.''J. Bacteriol.'' '''171''':5479-86</span>
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!align=left  |Citation
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'''No author listed'''  (1989) Cloning of the altered mRNA stability (ams) gene of Escherichia coli K-12. ''J. Bacteriol.'' '''171''':5479-86
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!align=left  |Abstract
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A temperature-sensitive mutation in the ams gene of Escherichia coli causes an increase in the chemical half-life of pulse-labeled RNA at the nonpermissive temperature. Using lambda clones containing DNA fragments from the 23- to 24-min region on the E. coli chromosome, we have isolated a 5.8-kilobase DNA fragment which, when present in a low-copy-number plasmid, complements the conditional lethality and increased mRNA stability associated with the ams-1 mutation. The approximate initiation site and the direction of transcription of the ams gene were determined from the size of truncated polypeptides produced by Tn1000 insertions and Bal 31 deletions. Overexpression of the ams locus by using a T7 RNA polymerase-promoter system permitted the identification of an ams-encoded polypeptide of 110 kilodaltons.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2477358 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC210387 PMC210387]


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|{{TableHeadingColor}} |'''Abstract''' || <protect>A temperature-sensitive mutation in the ams gene of Escherichia coli causes an increase in the chemical half-life of pulse-labeled RNA at the nonpermissive temperature. Using lambda clones containing DNA fragments from the 23- to 24-min region on the E. coli chromosome, we have isolated a 5.8-kilobase DNA fragment which, when present in a low-copy-number plasmid, complements the conditional lethality and increased mRNA stability associated with the ams-1 mutation. The approximate initiation site and the direction of transcription of the ams gene were determined from the size of truncated polypeptides produced by Tn1000 insertions and Bal 31 deletions. Overexpression of the ams locus by using a T7 RNA polymerase-promoter system permitted the identification of an ams-encoded polypeptide of 110 kilodaltons.</protect>
!align=left  |Keywords
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|{{TableHeadingColor}} nowrap |'''Links''' ||  [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2477358 PubMed]<br/>
Blotting, Southern; Cloning, Molecular; DNA Mutational Analysis; DNA Transposable Elements; DNA, Bacterial/genetics; Escherichia coli/genetics; Genes, Bacterial; Genetic Complementation Test; Molecular Weight; Nucleic Acid Hybridization; RNA, Bacterial/metabolism; RNA, Messenger/metabolism; Restriction Mapping; Ribonucleases/genetics
[http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2477358 Full text]
 
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==Significance {{HelpIcon|Help:Literature}} ==
==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}
{{LitSignificance}}

Revision as of 19:17, 1 September 2013

Citation

No author listed (1989) Cloning of the altered mRNA stability (ams) gene of Escherichia coli K-12. J. Bacteriol. 171:5479-86

Abstract

A temperature-sensitive mutation in the ams gene of Escherichia coli causes an increase in the chemical half-life of pulse-labeled RNA at the nonpermissive temperature. Using lambda clones containing DNA fragments from the 23- to 24-min region on the E. coli chromosome, we have isolated a 5.8-kilobase DNA fragment which, when present in a low-copy-number plasmid, complements the conditional lethality and increased mRNA stability associated with the ams-1 mutation. The approximate initiation site and the direction of transcription of the ams gene were determined from the size of truncated polypeptides produced by Tn1000 insertions and Bal 31 deletions. Overexpression of the ams locus by using a T7 RNA polymerase-promoter system permitted the identification of an ams-encoded polypeptide of 110 kilodaltons.

Links

PubMed PMC210387

Keywords

Blotting, Southern; Cloning, Molecular; DNA Mutational Analysis; DNA Transposable Elements; DNA, Bacterial/genetics; Escherichia coli/genetics; Genes, Bacterial; Genetic Complementation Test; Molecular Weight; Nucleic Acid Hybridization; RNA, Bacterial/metabolism; RNA, Messenger/metabolism; Restriction Mapping; Ribonucleases/genetics

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Annotations

Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status
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