SeqSearcher: Difference between revisions
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!align=left {{table heading style}} |Link/URL: | |||
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[http://ecoliwiki.net/tools/seqsearcher/index.php SeqSearcher] | |||
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!align=left {{table heading style}} |What: | |||
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Finds restriction sites that can be engineered into a gene without changing the amino acid sequence | |||
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!align=left {{table heading style}} |Who: | |||
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== Short Description == | == Short Description == | ||
[[Image:Seqsearch_home.jpg|right|thumb|300px|Home Page]] | |||
{{UserContentHelp}} | {{UserContentHelp}} | ||
Seqsearcher takes a polypeptide sequence and returns positions where silent mutations can be introduced to create restriction sites. Since it can't tell what the real DNA sequence is, it will also find real restriction sites. This is useful for generating cloning sites for cassette mutagenesis<ref name='PMID:1779849'/>. Cassette mutagenesis based on cloning between restriction sites has largely been superceded by PCR methods, but the software is available. | Seqsearcher takes a polypeptide sequence and returns positions where silent mutations can be introduced to create restriction sites. Since it can't tell what the real DNA sequence is, it will also find real restriction sites. This is useful for generating cloning sites for cassette mutagenesis<ref name='PMID:1779849'/>. Cassette mutagenesis based on cloning between restriction sites has largely been superceded by PCR methods, but the software is available. | ||
Latest revision as of 22:16, 17 June 2010
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| What: |
Finds restriction sites that can be engineered into a gene without changing the amino acid sequence |
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Short Description
Share your knowledge and ideas. How you can help. See Help pages if you need help with using the wiki
Seqsearcher takes a polypeptide sequence and returns positions where silent mutations can be introduced to create restriction sites. Since it can't tell what the real DNA sequence is, it will also find real restriction sites. This is useful for generating cloning sites for cassette mutagenesis[1]. Cassette mutagenesis based on cloning between restriction sites has largely been superceded by PCR methods, but the software is available.
Because the technique is not widely used anymore, further development of SeqSearcher is not being actively pursued.
Links
Seqsearcher can be run via a web form at http://ecoliwiki.net/tools/seqsearcher/
Requirements
The current version of SeqSearcher is a php script and is largely platform independent. The code can be downloaded here.
User notes
See Also
Similar functionality is available from other sequence analysis packages.
References
See Help:References for how to manage references in EcoliWiki.