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{{Tab On|Quickview|amtB}}{{Tab|Gene|amtB}}{{Tab|Gene Product(s)|amtB}}{{Tab|Expression|amtB}}{{Tab|Evolution|amtB}}{{Tab|On One Page|amtB}}{{TabsBottom}}
{{Tab On|Quickview|amtB}}{{Tab|Gene|amtB}}{{Tab|Gene Product(s)|amtB}}{{Tab|Expression|amtB}}{{Tab|Evolution|amtB}}{{Tab|On One Page|amtB}}{{TabsBottom}}
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!{{table_heading_style}} |Gene Name
!align=left {{table_heading_style}} |Standard Name
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[[amtB:Gene|''amtB]]'' (synonyms: ''ECK0445, b0451, JW0441, ybaG'')<ref name='LIB:Riley_2006'/>
''amtB''
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!{{table_heading_style}} |Product(s)  
!align=left {{table_heading_style}} |Gene Synonym(s)
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[[amtB:Gene_Product(s)|AmtB product]]<ref name='LIB:Riley_2006'/>: ammonium transporter<ref name='LIB:Riley_2006'/>, B0451<ref name='LIB:EcoCyc10.6'/>, YbaG<ref name='LIB:EcoCyc10.6'/>, AmtB<ref name='LIB:EcoCyc10.6'/>, AmtB ammonium Amt transporter<ref name='LIB:EcoCyc11.1'/>, [[:Category:Complex:ammonium Amt transporter|ammonium Amt transporter]]<ref name='LIB:EcoCyc11.1'/>
''ECK0445, b0451, JW0441, ybaG''<ref name='LIB:Riley_2006'/>, ybaG''
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!{{table_heading_style}} |Description
!align=left {{table_heading_style}} |Product Desc.
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AmtB ammonium Amt transporter<ref name='LIB:EcoCyc10.6'/>AmtB<ref name='LIB:EcoCyc11.1'/>
AmtB ammonium Amt transporter<ref name='LIB:EcoCyc10.6'/>; AmtB<ref name='LIB:EcoCyc11.1'/>;


Component of [[:Category:Complex:ammonium Amt transporter|ammonium Amt transporter]]<ref name='LIB:EcoCyc11.1'/>


Ammonia gas channel; sequesters GlnK, a negative regulator of AmtB activity, to the inner membrane; bi-directional facilitated diffusion<ref name='LIB:EcoGene'/>
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!align=left {{table_heading_style}} |Product Synonyms(s)
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''ammonium transporter<ref name='LIB:Riley_2006'/>, B0451<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, YbaG<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, AmtB<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, AmtB ammonium Amt transporter<ref name='LIB:EcoCyc11.1'/><ref name='LIB:Riley_2006'/> , ECK0445, JW0441, ybaG, b0451''
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!align=left {{table_heading_style}} |Function from GO
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<GO_nr />
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!{{table_heading_style}} |Knock-Out Phenotype
!align=left {{table_heading_style}} |Knock-Out Phenotype
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!{{table_heading_style}} |Regulation/Expression
!align=left {{table_heading_style}} |Regulation/Expression
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transcription unit(s): [[:Category:txn_units:glnK-amtB|glnK-amtB]]<ref name='LIB:EcoCyc10.6'/>  
transcription unit(s): [[:Category:Txn_unit:glnK-amtB|glnK-amtB]]<ref name='LIB:EcoCyc10.6'/>  
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!{{table_heading_style}} |Regulation/Activity
!align=left {{table_heading_style}} |Regulation/Activity
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!align=left {{table_heading_style}} |Quick Links
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[[File:porteco.png|link=http://porteco.org/AjaxSearch.jsp?searchString=amtB&s=search]] [[File:EcoCyc.gif|link=http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG11821]] [[File:regulondb.jpg|link=http://regulondb.ccg.unam.mx/gene?term=ECK120001753&organism=ECK12&format=jsp&type=gene ]] <br/><br/> [[File:dnadisplay.png|link=http://ecoliwiki.net/tools/display_dna.php?gene=amtB&loc=MG1655%3A472190..473476,W3110%3A472190..473476,DH10B%3A411521..412807,REL606%3A445033..446319,BW2952%3A374949..376235]] [[File:proteindisplay.png|link=http://ecoliwiki.net/tools/display_protein.php?f=NC_000913&start=374949&end=376235&gene=amtB&aa=mkiatiktglaslamlpglvmaapavadkadnafmmictalvlfmtipgialfygglirgknvlsmltqvtvtfalvcilwvvygyslafgegnnffgninwlmlknieltavmgsiyqyihvafqgsfacitvglivgalaerirfsavlifvvvwltlsyipiahmvwgggllashgaldfaggtvvhinaaiaglvgayligkrvgfgkeafkphnlpmvftgtailyigwfgfnagsagtaneiaalafvntvvataaailgwifgewalrgkpsllgacsgaiaglvgvtpacgyigvggaliigvvaglaglwgvtmlkrllrvddpcdvfgvhgvcgivgcimtgifaasslggvgfaegvtmghqllvqlesiaitivwsgvvafigykladltvglrvpeeqeregldvnshgenayna]]&nbsp;&nbsp;  [[File:pubmed.jpg|link=http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=Search&Term=amtB+AND+escherichia+coli]]&nbsp;&nbsp;  [[File:textpresso.jpg|link=http://textpresso.tamu.edu/cgi-bin/textpresso/search?q=amtB]]&nbsp;&nbsp;
 
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==Notes==
===Notes===
{{BacktoTop}}
 
AmtB is a trimeric inner membrane protein. Each subunit has 11 TM helices; a predicted N-terminal 12th TM helix is actually a cleaved signal peptide. Ammonium is proposed to bind  to the periplasmic face of AmtB and then to be deprotonated to ammonia for diffusion through the AmtB channel, then reprotonated by water to hydrate the ammonia gas to ammonium ion in the cytoplasm. AmtB enables growth on ammonium as sole nitrogen source at low ammonium ion concentrations (Soupene, 1998). The human Rh proteins are AmtB homologs and have been variously proposed to act as ammonia channels or as carbon dioxide gas channels.<ref name='LIB:EcoGene'/>
 




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==References==
==References==
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Latest revision as of 21:55, 27 May 2013

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
References Suggestions
Standard Name

amtB

Gene Synonym(s)

ECK0445, b0451, JW0441, ybaG[1], ybaG

Product Desc.

AmtB ammonium Amt transporter[2]; AmtB[3];

Component of ammonium Amt transporter[3]

Ammonia gas channel; sequesters GlnK, a negative regulator of AmtB activity, to the inner membrane; bi-directional facilitated diffusion[4]

Product Synonyms(s)

ammonium transporter[1], B0451[2][1], YbaG[2][1], AmtB[2][1], AmtB ammonium Amt transporter[3][1] , ECK0445, JW0441, ybaG, b0451

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): glnK-amtB[2]

Regulation/Activity
Quick Links



        

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Notes

[back to top]


AmtB is a trimeric inner membrane protein. Each subunit has 11 TM helices; a predicted N-terminal 12th TM helix is actually a cleaved signal peptide. Ammonium is proposed to bind to the periplasmic face of AmtB and then to be deprotonated to ammonia for diffusion through the AmtB channel, then reprotonated by water to hydrate the ammonia gas to ammonium ion in the cytoplasm. AmtB enables growth on ammonium as sole nitrogen source at low ammonium ion concentrations (Soupene, 1998). The human Rh proteins are AmtB homologs and have been variously proposed to act as ammonia channels or as carbon dioxide gas channels.[4]



References

[back to top]


  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
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