PMID:9190822: Difference between revisions

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<span style = 'font-size: large'> '''Franklund, CV and Kadner, RJ'''  (1997) Multiple transcribed elements control expression of the Escherichia coli btuB gene.''J. Bacteriol.'' '''179''':4039-42</span>
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!align=left  |Citation
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'''Franklund, CV and Kadner, RJ'''  (1997) Multiple transcribed elements control expression of the Escherichia coli btuB gene. ''J. Bacteriol.'' '''179''':4039-42
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!align=left  |Abstract
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Repression by vitamin B12 of the cobalamin transport protein BtuB in the outer membrane of Escherichia coli operates at both the transcriptional and translational levels and is controlled by transcribed sequences within the leader and proximal portion of the btuB coding sequence. The effects of deletions from either end of this region on repression and expression were determined with lac fusions. An element at the 5' end of the transcript and the putative attenuator within the coding sequence were required for transcriptional repression. The presence of either element caused a marked reduction in btuB-lacZ expression which was reversed by the presence of a conserved sequence element in the leader, suggesting the importance of long-range interactions in the btuB leader for expression and regulation.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9190822 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC179215 PMC179215]


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|{{TableHeadingColor}} |'''Abstract''' || Repression by vitamin B12 of the cobalamin transport protein BtuB in the outer membrane of Escherichia coli operates at both the transcriptional and translational levels and is controlled by transcribed sequences within the leader and proximal portion of the btuB coding sequence. The effects of deletions from either end of this region on repression and expression were determined with lac fusions. An element at the 5' end of the transcript and the putative attenuator within the coding sequence were required for transcriptional repression. The presence of either element caused a marked reduction in btuB-lacZ expression which was reversed by the presence of a conserved sequence element in the leader, suggesting the importance of long-range interactions in the btuB leader for expression and regulation.
!align=left  |Keywords
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Bacterial Outer Membrane Proteins; Escherichia coli/genetics; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Genes, Bacterial; Genes, Regulator; Membrane Transport Proteins; RNA, Messenger/analysis; Receptors, Peptide/genetics
|{{TableHeadingColor}} nowrap |'''Links''' ||  [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9190822 PubMed]
 
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==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
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Latest revision as of 04:57, 15 September 2013

Citation

Franklund, CV and Kadner, RJ (1997) Multiple transcribed elements control expression of the Escherichia coli btuB gene. J. Bacteriol. 179:4039-42

Abstract

Repression by vitamin B12 of the cobalamin transport protein BtuB in the outer membrane of Escherichia coli operates at both the transcriptional and translational levels and is controlled by transcribed sequences within the leader and proximal portion of the btuB coding sequence. The effects of deletions from either end of this region on repression and expression were determined with lac fusions. An element at the 5' end of the transcript and the putative attenuator within the coding sequence were required for transcriptional repression. The presence of either element caused a marked reduction in btuB-lacZ expression which was reversed by the presence of a conserved sequence element in the leader, suggesting the importance of long-range interactions in the btuB leader for expression and regulation.

Links

PubMed PMC179215

Keywords

Bacterial Outer Membrane Proteins; Escherichia coli/genetics; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Genes, Bacterial; Genes, Regulator; Membrane Transport Proteins; RNA, Messenger/analysis; Receptors, Peptide/genetics

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