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{{Tab On|Quickview|dcm}}{{Tab|Gene|dcm}}{{Tab|Gene Product(s)|dcm}}{{Tab|Expression|dcm}}{{Tab|Evolution|dcm}}{{Tab|On One Page|dcm}}{{TabsBottom}}
{{Tab On|Quickview|dcm}}{{Tab|Gene|dcm}}{{Tab|Gene Product(s)|dcm}}{{Tab|Expression|dcm}}{{Tab|Evolution|dcm}}{{Tab|On One Page|dcm}}{{TabsBottom}}
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!{{table_heading_style}} |Gene Name
!align=left {{table_heading_style}} |Standard Name
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[[dcm:Gene|''dcm]]'' (synonyms: ''ECK1959, b1961, JW1944, mec'')<ref name='LIB:Riley_2006'/>
''dcm''
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!{{table_heading_style}} |Product(s)  
!align=left {{table_heading_style}} |Gene Synonym(s)
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[[dcm:Gene_Product(s)|Dcm product]]<ref name='LIB:Riley_2006'/>: DNA cytosine methylase<ref name='LIB:Riley_2006'/>, B1961<ref name='LIB:EcoCyc10.6'/>, Mec<ref name='LIB:EcoCyc10.6'/>, Dcm<ref name='LIB:EcoCyc10.6'/>
''ECK1959, b1961, JW1944, mec''<ref name='LIB:Riley_2006'/>, mec''
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!{{table_heading_style}} |Description
!align=left {{table_heading_style}} |Product Desc.
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DNA cytosine methylase<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>
DNA cytosine methylase<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>


 
DNA cytosine methyltransferase; internal cytosine methylated<ref name='LIB:EcoGene'/>
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!align=left {{table_heading_style}} |Product Synonyms(s)
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''DNA cytosine methylase<ref name='LIB:Riley_2006'/>, B1961<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, Mec<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, Dcm<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/> , ECK1959, JW1944, mec, b1961''
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!align=left {{table_heading_style}} |Function from GO
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<GO_nr />
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!{{table_heading_style}} |Knock-Out Phenotype
!align=left {{table_heading_style}} |Knock-Out Phenotype
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!{{table_heading_style}} |Regulation/Expression
!align=left {{table_heading_style}} |Regulation/Expression
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transcription unit(s): [[:Category:txn_units:dcm-vsr|dcm-vsr]]<ref name='LIB:EcoCyc10.6'/>  
transcription unit(s): [[:Category:Txn_unit:dcm-vsr|dcm-vsr]]<ref name='LIB:EcoCyc10.6'/>  
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!{{table_heading_style}} |Regulation/Activity
!align=left {{table_heading_style}} |Regulation/Activity
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[[File:porteco.png|link=http://porteco.org/AjaxSearch.jsp?searchString=dcm&s=search]] [[File:EcoCyc.gif|link=http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10211]] [[File:regulondb.jpg|link=http://regulondb.ccg.unam.mx/gene?term=ECK120000205&organism=ECK12&format=jsp&type=gene ]] <br/><br/> [[File:dnadisplay.png|link=http://ecoliwiki.net/tools/display_dna.php?gene=dcm&loc=MG1655%3A2030341..2028923,W3110%3A2034454..2033036,DH10B%3A2121349..2119931,BW2952%3A1921406..1922824]] [[File:proteindisplay.png|link=http://ecoliwiki.net/tools/display_protein.php?f=NC_000913&start=1921406&end=1922824&gene=dcm&aa=mqenisvtdsystgnaaqamlekllqiydvktlvaqlngvgenhwsaailkralandsawhrlsekefahlqtllpkppahhphyafrfidlfagiggirrgfesiggqcvftsewnkhavrtykanhycdpathhfnedirditlshkegvsdeaaaehirqhipehdvllagfpcqpfslagvskknslgrahgfacdtqgtlffdvvriidarrpamfvlenvknlkshdqgktfriimqtldelgydvadaedngpddpkiidgkhflpqhrerivlvgfrrdlnlkadftlrdisecfpaqrvtlaqlldpmveakyiltpvlwkylyryakkhqargngfgygmvypnnpqsvtrtlsaryykdgaeilidrgwdmatgekdfddplnqqhrprrltprecarlmgfeapgeakfripvsdtqayrqfgnsvvvpvfaavakllepkikqavalrqqeaqhgrrsr]]&nbsp;&nbsp;  [[File:pubmed.jpg|link=http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=Search&Term=dcm+AND+escherichia+coli]]&nbsp;&nbsp;  [[File:textpresso.jpg|link=http://textpresso.tamu.edu/cgi-bin/textpresso/search?q=dcm]]&nbsp;&nbsp;
 
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==Notes==
===Notes===
 
'''Method of Mutagenesis:''' Log-phase cultures of strain GM 1 were treated with Alkylating agent (N-methyl-N-nitro-nitrosoguanidine)<ref name=PMID:4576399/>
 
 
'''Phenotype Observed:''' Mutants were tested for S-adenosylmethionine methyltransferase activity by measuring the levels of DNA-cytosine (S-adenosylmethionine and DNA-adenine.  The mutants showed reduced or absent activity.  Also, deficient in methylcytosine in DNA. <ref name=PMID:4576399/>
 
 
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==References==
==References==
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Latest revision as of 21:51, 27 May 2013

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
References Suggestions
Standard Name

dcm

Gene Synonym(s)

ECK1959, b1961, JW1944, mec[1], mec

Product Desc.

DNA cytosine methylase[2][3]

DNA cytosine methyltransferase; internal cytosine methylated[4]

Product Synonyms(s)

DNA cytosine methylase[1], B1961[2][1], Mec[2][1], Dcm[2][1] , ECK1959, JW1944, mec, b1961

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): dcm-vsr[2]

Regulation/Activity
Quick Links



        

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Notes

Method of Mutagenesis: Log-phase cultures of strain GM 1 were treated with Alkylating agent (N-methyl-N-nitro-nitrosoguanidine)[5]


Phenotype Observed: Mutants were tested for S-adenosylmethionine methyltransferase activity by measuring the levels of DNA-cytosine (S-adenosylmethionine and DNA-adenine. The mutants showed reduced or absent activity. Also, deficient in methylcytosine in DNA. [5]


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References

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  1. ↑ 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 Marinus, MG & Morris, NR (1973) Isolation of deoxyribonucleic acid methylase mutants of Escherichia coli K-12. J. Bacteriol. 114 1143-50 PubMed
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