PMID:3025182: Difference between revisions

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<span style = 'font-size: large'> '''Chye, ML and Pittard, J'''  (1987) Transcription control of the aroP gene in Escherichia coli K-12: analysis of operator mutants.''J. Bacteriol.'' '''169''':386-93</span>
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!align=left  |Citation
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'''Chye, ML and Pittard, J'''  (1987) Transcription control of the aroP gene in Escherichia coli K-12: analysis of operator mutants. ''J. Bacteriol.'' '''169''':386-93
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!align=left  |Abstract
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The nucleotide sequence of the region containing the promoter-operator for the aroP gene was determined. The start site of aroP transcription was identified by using S1 nuclease mapping and primer extension techniques. Examination of the nucleotide sequence revealed the presence of two "TYR R" boxes which are similar to those identified in the regulatory regions of other genes in the tyrR regulon. Bisulfite-induced aroP operator-constitutive mutants were analyzed, and the base-pair changes responsible for alterations in aroP regulation were located within these boxes.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3025182 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC211779 PMC211779]


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|{{TableHeadingColor}} |'''Abstract''' || The nucleotide sequence of the region containing the promoter-operator for the aroP gene was determined. The start site of aroP transcription was identified by using S1 nuclease mapping and primer extension techniques. Examination of the nucleotide sequence revealed the presence of two "TYR R" boxes which are similar to those identified in the regulatory regions of other genes in the tyrR regulon. Bisulfite-induced aroP operator-constitutive mutants were analyzed, and the base-pair changes responsible for alterations in aroP regulation were located within these boxes.
!align=left  |Keywords
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Acetyltransferases/metabolism; Base Sequence; Chloramphenicol O-Acetyltransferase; Electrophoresis, Polyacrylamide Gel; Endonucleases/metabolism; Escherichia coli/genetics; Genes, Bacterial; Mutation; Operon; Promoter Regions, Genetic; Single-Strand Specific DNA and RNA Endonucleases; Sulfites/pharmacology; Transcription, Genetic
|{{TableHeadingColor}} nowrap |'''Links''' ||  [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3025182 PubMed]
 
[http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=3025182 Full text]
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==Annotations {{HelpIcon|Help:Literature}}==
==Annotations {{HelpIcon|Help:Literature}}==
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==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
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Latest revision as of 04:45, 15 September 2013

Citation

Chye, ML and Pittard, J (1987) Transcription control of the aroP gene in Escherichia coli K-12: analysis of operator mutants. J. Bacteriol. 169:386-93

Abstract

The nucleotide sequence of the region containing the promoter-operator for the aroP gene was determined. The start site of aroP transcription was identified by using S1 nuclease mapping and primer extension techniques. Examination of the nucleotide sequence revealed the presence of two "TYR R" boxes which are similar to those identified in the regulatory regions of other genes in the tyrR regulon. Bisulfite-induced aroP operator-constitutive mutants were analyzed, and the base-pair changes responsible for alterations in aroP regulation were located within these boxes.

Links

PubMed PMC211779

Keywords

Acetyltransferases/metabolism; Base Sequence; Chloramphenicol O-Acetyltransferase; Electrophoresis, Polyacrylamide Gel; Endonucleases/metabolism; Escherichia coli/genetics; Genes, Bacterial; Mutation; Operon; Promoter Regions, Genetic; Single-Strand Specific DNA and RNA Endonucleases; Sulfites/pharmacology; Transcription, Genetic

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