pThioHis C: Difference between revisions
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==At a Glance== | ==At a Glance== | ||
[[ | __NOTOC__ | ||
[[File:PThioHis C.jpg|thumb|450px|pThioHis C pMOD-3, Image made with [[PlasMapper]]]] | |||
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{|{{Prettytable}} width='30%' align=left | {| {{Prettytable}} width='30%' align=left id="R470af5383e6e0" class=" tableEdit PlasmidTable" | ||
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!{{table heading style}} |Type | !align=left {{table heading style}} |Type | ||
| | || | ||
Expression | Expression | ||
|- | |- | ||
!{{table heading style}} |Origin of Replication | !align=left {{table heading style}} |Origin of Replication | ||
| | || | ||
pUC | pUC | ||
|- | |- | ||
!{{table heading style}} |Copy # | !align=left {{table heading style}} |Copy # | ||
| | || | ||
|- | |- | ||
!{{table heading style}} |Markers | !align=left {{table heading style}} |Markers | ||
| | || | ||
Ampicillin | Ampicillin | ||
|- | |- | ||
!{{table heading style}} |Link to Sequence | !align=left {{table heading style}} |Link to Sequence | ||
| | || | ||
[http://www.invitrogen.com/content/sfs/vectors/pthiohisc_seq.txt Invitrogen] | [http://www.invitrogen.com/content/sfs/vectors/pthiohisc_seq.txt Invitrogen] | ||
|- | |- | ||
!{{table heading style}} |Features | !align=left {{table heading style}} |Features | ||
| | || | ||
Lac Repressor (lacIq) ORF<br> | Lac Repressor (lacIq) ORF<br /> | ||
Trc Promotor Region<br> | Trc Promotor Region<br /> | ||
lac operator (lacO)<br> | lac operator (lacO)<br /> | ||
rrnB antitermination<br> | rrnB antitermination<br /> | ||
gene 10 region<br> | gene 10 region<br /> | ||
Ribosome binding site<br> | Ribosome binding site<br /> | ||
Thioredoxin ATG<br> | Thioredoxin ATG<br /> | ||
Trx forward priming site<br> | Trx forward priming site<br /> | ||
Enterokinase site<br> | Enterokinase site<br /> | ||
Multiple cloning site<br> | Multiple cloning site<br /> | ||
Sph I<br> | Sph I<br /> | ||
aspA Termination<br> | aspA Termination<br /> | ||
Trx reverse priming site | Trx reverse priming site | ||
|- | |- | ||
!{{table heading style}} |Accession Number | !align=left {{table heading style}} |Accession Number | ||
| | || | ||
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==Notes== | ==Notes== | ||
pThioHis vectors include a modified thioredoxin gene for the purification of fusion proteins with metal-chelating resins and an enterokinase cleavage site for efficient cleavage of N-terminal fusions. Sph I in multiple cloning site is unique to pThioHis C. | pThioHis vectors include a modified thioredoxin gene for the purification of fusion proteins with metal-chelating resins and an enterokinase cleavage site for efficient cleavage of N-terminal fusions. Sph I in multiple cloning site is unique to pThioHis C. | ||
| Line 62: | Line 66: | ||
==Sources== | ==Sources== | ||
[http://www.invitrogen.com/search/ | [http://www.invitrogen.com/search/global/searchAction.action?query=pThioHis+C&resultPage=1&resultsPerPage=15&x=25&y=4 Invitrogen] | ||
==References== | ==References== | ||
{{BacktoTop}} | |||
{{RefHelp}} | {{RefHelp}} | ||
<references/> | <references /> | ||
==Sequence== | ==Sequence== | ||
Latest revision as of 17:03, 30 August 2011
At a Glance
| Type |
Expression |
|---|---|
| Origin of Replication |
pUC |
| Copy # | |
| Markers |
Ampicillin |
| Link to Sequence | |
| Features |
Lac Repressor (lacIq) ORF |
| Accession Number |
|
| edit table |
Notes
pThioHis vectors include a modified thioredoxin gene for the purification of fusion proteins with metal-chelating resins and an enterokinase cleavage site for efficient cleavage of N-terminal fusions. Sph I in multiple cloning site is unique to pThioHis C.
History
pThioHis Vectors are intended for use with the His-Patch ThioFusion™ Expression System.
Sources
References
See Help:References for how to manage references in EcoliWiki.
Sequence
GACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTC AGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATG TATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTC AACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAAC GCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGC TATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCA GAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTA TGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGA AGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCT GAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAA CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAG TTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGA GCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTAC ACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTA AGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATT TAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTC CACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCT GCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCT TTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTA GGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTG CTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCG GTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATAC CTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCG GCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGT CGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA AACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTTACGTTGA CACCATCGAATGGTGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTG GTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCC GCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCT GAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACC TCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTG CCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCT CGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCT GCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCT CCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTT AGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAAT CAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAA TGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCG CGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGAC AGCTCATGTTATATCCCGCCGTCAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGG ACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAA AAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAG CTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCGCGAA TTGATCTGGTTTGACAGCTTATCATCGACTGCACGGTGCACCAATGCTTCTGGCGTCAGGCAGCCATCGG AAGCTGTGGTATGGCTGTGCAGGTCGTAAATCACTGCATAATTCGTGTCGCTCAAGGCGCACTCCCGTTC TGGATAATGTTTTTTGCGCCGACATCATAACGGTTCTGGCAAATATTCTGAAATGAGCTGTTGACAATTA ATCATCCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCGCCGCTGA GAAAAAGCGAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGACCGGAATTAT CGATTAACTTTATTATTAAAAATTAAAGAGGTATATACATATGTCTGATAAAATTATTCATCTGACTGAT GATTCTTTTGATACTGATGTACTTAAGGCAGATGGTGCAATCCTGGTTGATTTCTGGGCACACTGGTGCG GTCCGTGCAAAATGATCGCTCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGC AAAACTGAACATCGATCACAACCCGGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTCTGCTG CTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCC TCGACGCTAACCTGGCCGGCTCTGGATCCGGTGATGACGATGACAAGGTACCTGGCATGCTGAGCTCGAG ATCTTCGAATTCCGCGGCCGCAGGCCTCTAGAGTCGACCTGCAGTAATCGTACAGGGTAGTACAAATAAA AAAGGCACGTCAGATGACGTGCCTTTTTTCTTGTGAGCAGTAAGCTTGGCACTGGCCGTCGTTTTACAAC GTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTG GCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGC CTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATATGGTGCACTCTCAGTAC AATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGG GCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGT TTTCACCGTCATCACCGAAACGCGCGA