PMID:6379647: Difference between revisions
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!align=left |Citation | |||
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'''Blanar, MA, Sandler, SJ, Armengod, ME, Ream, LW and Clark, AJ''' (1984) Molecular analysis of the recF gene of Escherichia coli. ''Proc. Natl. Acad. Sci. U.S.A.'' '''81''':4622-6 | |||
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!align=left |Abstract | |||
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We analyzed the nucleotide sequence of a 1.325-kilobase region of wild-type Escherichia coli containing a functional recF gene and six Tn3 mutations that inactivate recF. The analysis shows a potentially translatable reading frame of 1071 nucleotides, which is interrupted by all six insertions. A protein of 40.5 kilodaltons would result from translation of the open reading frame, and a radioactive band of protein of an apparent molecular weight of approximately 40 kilodaltons was seen by the maxicell method using a recF+ plasmid. Putative truncated peptides were seen when two recF::Tn3 mutant plasmids were used. Differential expression of dnaN and recF from a common promoter was noted. recF332::Tn3 was transferred to the chromosome where, in hemizygous condition, it produced UV sensitivity indistinguishable from that produced by two presumed recF point mutations. | |||
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6379647 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC391542 PMC391542] | |||
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| | !align=left |Keywords | ||
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|{{ | Amino Acid Sequence; Bacterial Proteins/genetics; Base Sequence; DNA Repair; DNA Replication; Escherichia coli/genetics; Genes, Bacterial; Molecular Weight; Mutation; Plasmids; Recombination, Genetic | ||
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==Significance {{HelpIcon|Help:Literature}} == | ==Significance {{HelpIcon|Help:Literature}} == | ||
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==Annotations {{HelpIcon|Help:Literature}}== | ==Annotations {{HelpIcon|Help:Literature}}== | ||
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==EcoliWiki Links {{HelpIcon|Help:Literature}} == | ==EcoliWiki Links {{HelpIcon|Help:Literature}} == | ||
Latest revision as of 04:49, 15 September 2013
| Citation |
Blanar, MA, Sandler, SJ, Armengod, ME, Ream, LW and Clark, AJ (1984) Molecular analysis of the recF gene of Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 81:4622-6 |
|---|---|
| Abstract |
We analyzed the nucleotide sequence of a 1.325-kilobase region of wild-type Escherichia coli containing a functional recF gene and six Tn3 mutations that inactivate recF. The analysis shows a potentially translatable reading frame of 1071 nucleotides, which is interrupted by all six insertions. A protein of 40.5 kilodaltons would result from translation of the open reading frame, and a radioactive band of protein of an apparent molecular weight of approximately 40 kilodaltons was seen by the maxicell method using a recF+ plasmid. Putative truncated peptides were seen when two recF::Tn3 mutant plasmids were used. Differential expression of dnaN and recF from a common promoter was noted. recF332::Tn3 was transferred to the chromosome where, in hemizygous condition, it produced UV sensitivity indistinguishable from that produced by two presumed recF point mutations. |
| Links | |
| Keywords |
Amino Acid Sequence; Bacterial Proteins/genetics; Base Sequence; DNA Repair; DNA Replication; Escherichia coli/genetics; Genes, Bacterial; Molecular Weight; Mutation; Plasmids; Recombination, Genetic |
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