PMID:12379844: Difference between revisions

From EcoliWiki
Jump to navigation Jump to search
← Older edit
Edited by wikibot
imported>Wikientrybot
change PMID template to TableEdit version
 
(6 intermediate revisions by 2 users not shown)
Line 1: Line 1:
{{RightTOC}}
{{RightTOC}}
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.118909.N5223842a77115-->
<!--
******************************************************************************************
*
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **
*
****************************************************************************************** -->
{|  id="N5223842a77115"  class=" tableEdit PMID_info_table" 


<span style = 'font-size: large'> '''Guarné, A, Zhao, Q, Ghirlando, R and Yang, W'''  (2002) Insights into negative modulation of E. coli replication initiation from the structure of SeqA-hemimethylated DNA complex.''Nat. Struct. Biol.'' '''9''':839-43</span>
{|{{Prettytable}} align = 'center' width= '70%'
|-
|-
|{{TableHeadingColor}} |'''Abstract''' || The SeqA protein binds clusters of fully methylated or hemimethylated GATC sequences at oriC and negatively modulates the initiation of DNA replication. We find that SeqA can be proteolytically cleaved into an N-terminal multimerization and a C-terminal DNA-binding domain and have determined the crystal structure of the C-terminal domain in complex with a hemimethylated GATC site. SeqA makes direct hydrogen bonds and van der Waals contacts with the hemimethylated A-T base pair in addition to interactions with the surrounding bases and DNA backbone. The tetrameric protein-DNA complex found in the crystal suggests that SeqA binds multiple GATC sites on separate DNA duplexes, altering the overall DNA topology and sequestering oriC from replication initiation.
!align=left  |Citation
</noinclude>
||
'''Guarné, A, Zhao, Q, Ghirlando, R and Yang, W'''  (2002) Insights into negative modulation of E. coli replication initiation from the structure of SeqA-hemimethylated DNA complex. ''Nat. Struct. Biol.'' '''9''':839-43
|-
!align=left  |Abstract
||
The SeqA protein binds clusters of fully methylated or hemimethylated GATC sequences at oriC and negatively modulates the initiation of DNA replication. We find that SeqA can be proteolytically cleaved into an N-terminal multimerization and a C-terminal DNA-binding domain and have determined the crystal structure of the C-terminal domain in complex with a hemimethylated GATC site. SeqA makes direct hydrogen bonds and van der Waals contacts with the hemimethylated A-T base pair in addition to interactions with the surrounding bases and DNA backbone. The tetrameric protein-DNA complex found in the crystal suggests that SeqA binds multiple GATC sites on separate DNA duplexes, altering the overall DNA topology and sequestering oriC from replication initiation.
|-
!align=left  |Links
||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12379844 PubMed]
Online version:[http://dx.doi.org/10.1038/nsb857 10.1038/nsb857]
|-
|-
|{{TableHeadingColor}} nowrap |'''Links''' ||  [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12379844 PubMed]
!align=left  |Keywords
||
Bacterial Outer Membrane Proteins; Crystallography, X-Ray; DNA Methylation; DNA Replication/physiology; DNA, Bacterial/biosynthesis; DNA, Bacterial/chemistry; DNA, Bacterial/metabolism; DNA-Binding Proteins; Escherichia coli/genetics; Escherichia coli/physiology; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/metabolism; Models, Molecular; Nucleic Acid Conformation; Protein Binding; Protein Structure, Tertiary; Recombinant Proteins/chemistry; Replication Origin; Transcription Factors/chemistry; Transcription Factors/metabolism
 
|- class="tableEdit_footer"
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.118909.N5223842a77115&page=118909&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table]</span> ||
|}
|}
 
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.118909.N5223842a77115-->
==Significance {{HelpIcon|Help:Literature}} ==
==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}
{{LitSignificance}}
Line 19: Line 38:


==Annotations {{HelpIcon|Help:Literature}}==
==Annotations {{HelpIcon|Help:Literature}}==
<!--box uid=63ce1649aab8b3180235b0af7618f667.0.U47a9778d16990-->
<annotationlinks/>
<!--
******************************************************************************************
*
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **
*
****************************************************************************************** -->
{|class = 'sortable' {{Prettytable}} id='226583'
|- {{table heading style}}
!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-class='sortbottom'
|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.U47a9778d16990&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || || ||
|}
<!--box uid=63ce1649aab8b3180235b0af7618f667.0.U47a9778d16990-->


==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
==EcoliWiki Links {{HelpIcon|Help:Literature}} ==

Latest revision as of 04:18, 15 September 2013

Citation

Guarné, A, Zhao, Q, Ghirlando, R and Yang, W (2002) Insights into negative modulation of E. coli replication initiation from the structure of SeqA-hemimethylated DNA complex. Nat. Struct. Biol. 9:839-43

Abstract

The SeqA protein binds clusters of fully methylated or hemimethylated GATC sequences at oriC and negatively modulates the initiation of DNA replication. We find that SeqA can be proteolytically cleaved into an N-terminal multimerization and a C-terminal DNA-binding domain and have determined the crystal structure of the C-terminal domain in complex with a hemimethylated GATC site. SeqA makes direct hydrogen bonds and van der Waals contacts with the hemimethylated A-T base pair in addition to interactions with the surrounding bases and DNA backbone. The tetrameric protein-DNA complex found in the crystal suggests that SeqA binds multiple GATC sites on separate DNA duplexes, altering the overall DNA topology and sequestering oriC from replication initiation.

Links

PubMed Online version:10.1038/nsb857

Keywords

Bacterial Outer Membrane Proteins; Crystallography, X-Ray; DNA Methylation; DNA Replication/physiology; DNA, Bacterial/biosynthesis; DNA, Bacterial/chemistry; DNA, Bacterial/metabolism; DNA-Binding Proteins; Escherichia coli/genetics; Escherichia coli/physiology; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/metabolism; Models, Molecular; Nucleic Acid Conformation; Protein Binding; Protein Structure, Tertiary; Recombinant Proteins/chemistry; Replication Origin; Transcription Factors/chemistry; Transcription Factors/metabolism

edit table

Significance

You can help EcoliWiki by summarizing why this paper is useful

Useful Materials and Methods

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Annotations

<annotationlinks/>

EcoliWiki Links

Add links to pages that link here (e.g. gene, product, method pages)

References

See Help:References for how to manage references in EcoliWiki.

Retrieved from "https://ecoliwiki.org/colipedia/index.php?title=PMID:12379844&oldid=1455506"