PMID:776928: Difference between revisions

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<span style = 'font-size: large'> '''Austin, S'''  (1976) Wild-type and mutant in vitro products of an operon for ribonucleic acid polymerase subunits.''J. Bacteriol.'' '''127''':32-9</span>
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!align=left  |Citation
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'''Austin, S'''  (1976) Wild-type and mutant in vitro products of an operon for ribonucleic acid polymerase subunits. ''J. Bacteriol.'' '''127''':32-9
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!align=left  |Abstract
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An in vitro protein-synthesizing system can synthesize two ribonucleic acid (RNA) polymerase subunits of Escherichia coli, beta and beta', when a transducing phage deoxyribonucleic acid (DNA) template containing the rpoB region of the bacterial chromosome is added. Recombinant rpoB transducing phages were isolated that carry "nonsense" mutations of the class rpo-rifampin zero amber (formally referred to as rifoam). DNA was extracted from two of these phages. These DNAs are unable to direct the synthesis of beta subunits, whereas beta' synthesis is unaffected. Both mutations can be efficiently suppressed in vitro by the addition of suppressor transfer RNA. One of the mutations (rpoB115) produces a detectable nonsense (or restart) fragment of the beta protein in the absence of suppression. It is concluded that rpoB115 is an amber mutation within the structural gene for the beta subunit of RNA polymerase.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=776928 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC233029 PMC233029]


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|{{TableHeadingColor}} |'''Abstract''' || <protect>An in vitro protein-synthesizing system can synthesize two ribonucleic acid (RNA) polymerase subunits of Escherichia coli, beta and beta', when a transducing phage deoxyribonucleic acid (DNA) template containing the rpoB region of the bacterial chromosome is added. Recombinant rpoB transducing phages were isolated that carry "nonsense" mutations of the class rpo-rifampin zero amber (formally referred to as rifoam). DNA was extracted from two of these phages. These DNAs are unable to direct the synthesis of beta subunits, whereas beta' synthesis is unaffected. Both mutations can be efficiently suppressed in vitro by the addition of suppressor transfer RNA. One of the mutations (rpoB115) produces a detectable nonsense (or restart) fragment of the beta protein in the absence of suppression. It is concluded that rpoB115 is an amber mutation within the structural gene for the beta subunit of RNA polymerase.</protect>
!align=left  |Keywords
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Cell-Free System; Coliphages/drug effects; Coliphages/growth & development; DNA, Viral; DNA-Directed RNA Polymerases/biosynthesis; Drug Resistance, Microbial; Escherichia coli/enzymology; Lysogeny; Mutation; Operon; Recombination, Genetic; Rifampin/pharmacology; Suppression, Genetic; Templates, Genetic; Transduction, Genetic
|{{TableHeadingColor}} nowrap |'''Links''' ||  [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=776928 PubMed]
 
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==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
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Latest revision as of 04:51, 15 September 2013

Citation

Austin, S (1976) Wild-type and mutant in vitro products of an operon for ribonucleic acid polymerase subunits. J. Bacteriol. 127:32-9

Abstract

An in vitro protein-synthesizing system can synthesize two ribonucleic acid (RNA) polymerase subunits of Escherichia coli, beta and beta', when a transducing phage deoxyribonucleic acid (DNA) template containing the rpoB region of the bacterial chromosome is added. Recombinant rpoB transducing phages were isolated that carry "nonsense" mutations of the class rpo-rifampin zero amber (formally referred to as rifoam). DNA was extracted from two of these phages. These DNAs are unable to direct the synthesis of beta subunits, whereas beta' synthesis is unaffected. Both mutations can be efficiently suppressed in vitro by the addition of suppressor transfer RNA. One of the mutations (rpoB115) produces a detectable nonsense (or restart) fragment of the beta protein in the absence of suppression. It is concluded that rpoB115 is an amber mutation within the structural gene for the beta subunit of RNA polymerase.

Links

PubMed PMC233029

Keywords

Cell-Free System; Coliphages/drug effects; Coliphages/growth & development; DNA, Viral; DNA-Directed RNA Polymerases/biosynthesis; Drug Resistance, Microbial; Escherichia coli/enzymology; Lysogeny; Mutation; Operon; Recombination, Genetic; Rifampin/pharmacology; Suppression, Genetic; Templates, Genetic; Transduction, Genetic

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