PMID:1459941: Difference between revisions

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<span style = 'font-size: large'> '''Santini, CL, Iobbi-Nivol, C, Romane, C, Boxer, DH and Giordano, G'''  (1992) Molybdoenzyme biosynthesis in Escherichia coli: in vitro activation of purified nitrate reductase from a chlB mutant.''J. Bacteriol.'' '''174''':7934-40</span>
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'''Santini, CL, Iobbi-Nivol, C, Romane, C, Boxer, DH and Giordano, G'''  (1992) Molybdoenzyme biosynthesis in Escherichia coli: in vitro activation of purified nitrate reductase from a chlB mutant. ''J. Bacteriol.'' '''174''':7934-40
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!align=left  |Abstract
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All molybdoenzyme activities are absent in chlB mutants because of their inability to synthesize molybdopterin guanine dinucleotide, which together with molybdate constitutes the molybdenum cofactor in Escherichia coli. The chlB mutants are able to synthesize molybdopterin. We have previously shown that the inactive nitrate reductase present in a chlB mutant can be activated in a process requiring protein FA and a heat-stable low-molecular-weight substance. We show here that purified nitrate reductase from the soluble fraction of a chlB mutant can be partially activated in a process that requires protein FA, GTP, and an additional protein termed factor X. It appears that the molybdopterin present in the nitrate reductase of a chlB mutant is converted to molybdopterin guanine dinucleotide during activation. The activation is absolutely dependent upon both protein FA and factor X. Factor X activity is present in chlA, chlB, chlE, and chlG mutants.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1459941 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC207528 PMC207528]


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|{{TableHeadingColor}} |'''Abstract''' || All molybdoenzyme activities are absent in chlB mutants because of their inability to synthesize molybdopterin guanine dinucleotide, which together with molybdate constitutes the molybdenum cofactor in Escherichia coli. The chlB mutants are able to synthesize molybdopterin. We have previously shown that the inactive nitrate reductase present in a chlB mutant can be activated in a process requiring protein FA and a heat-stable low-molecular-weight substance. We show here that purified nitrate reductase from the soluble fraction of a chlB mutant can be partially activated in a process that requires protein FA, GTP, and an additional protein termed factor X. It appears that the molybdopterin present in the nitrate reductase of a chlB mutant is converted to molybdopterin guanine dinucleotide during activation. The activation is absolutely dependent upon both protein FA and factor X. Factor X activity is present in chlA, chlB, chlE, and chlG mutants.
!align=left  |Keywords
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Bacterial Proteins/metabolism; Chlorates/pharmacology; Coenzymes; Drug Resistance, Microbial/genetics; Enzyme Activation; Escherichia coli/drug effects; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/metabolism; Guanosine Triphosphate/metabolism; Kinetics; Metalloproteins/metabolism; Molecular Weight; Mutation; Nitrate Reductase; Nitrate Reductases/genetics; Nitrate Reductases/metabolism; Pteridines/metabolism; Spectrometry, Fluorescence
|{{TableHeadingColor}} nowrap |'''Links''' ||  [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1459941 PubMed]
 
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Latest revision as of 04:22, 15 September 2013

Citation

Santini, CL, Iobbi-Nivol, C, Romane, C, Boxer, DH and Giordano, G (1992) Molybdoenzyme biosynthesis in Escherichia coli: in vitro activation of purified nitrate reductase from a chlB mutant. J. Bacteriol. 174:7934-40

Abstract

All molybdoenzyme activities are absent in chlB mutants because of their inability to synthesize molybdopterin guanine dinucleotide, which together with molybdate constitutes the molybdenum cofactor in Escherichia coli. The chlB mutants are able to synthesize molybdopterin. We have previously shown that the inactive nitrate reductase present in a chlB mutant can be activated in a process requiring protein FA and a heat-stable low-molecular-weight substance. We show here that purified nitrate reductase from the soluble fraction of a chlB mutant can be partially activated in a process that requires protein FA, GTP, and an additional protein termed factor X. It appears that the molybdopterin present in the nitrate reductase of a chlB mutant is converted to molybdopterin guanine dinucleotide during activation. The activation is absolutely dependent upon both protein FA and factor X. Factor X activity is present in chlA, chlB, chlE, and chlG mutants.

Links

PubMed PMC207528

Keywords

Bacterial Proteins/metabolism; Chlorates/pharmacology; Coenzymes; Drug Resistance, Microbial/genetics; Enzyme Activation; Escherichia coli/drug effects; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/metabolism; Guanosine Triphosphate/metabolism; Kinetics; Metalloproteins/metabolism; Molecular Weight; Mutation; Nitrate Reductase; Nitrate Reductases/genetics; Nitrate Reductases/metabolism; Pteridines/metabolism; Spectrometry, Fluorescence

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