PMID:2139644: Difference between revisions

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<span style = 'font-size: large'> '''Lichens-Park, A, Smith, CL and Syvanen, M'''  (1990) Integration of bacteriophage lambda into the cryptic lambdoid prophages of Escherichia coli.''J. Bacteriol.'' '''172''':2201-8</span>
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!align=left  |Citation
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'''Lichens-Park, A, Smith, CL and Syvanen, M'''  (1990) Integration of bacteriophage lambda into the cryptic lambdoid prophages of Escherichia coli. ''J. Bacteriol.'' '''172''':2201-8
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!align=left  |Abstract
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Bacteriophage lambda missing its chromosomal attachment site will integrate into recA+ Escherichia coli K-12 and C at the sites of cryptic prophages. The specific regions in which these recombination events occur were identified in both lambda and the bacterial chromosomes. A NotI restriction site on the prophage allowed its physical mapping. This allowed us to identify the locations of Rac, Qin, and Qsr' cryptic prophages on the NotI map of E. coli K-12 and, by analogy, to identify the cryptic prophage in E. coli C as Qin. No new cryptic prophages were detected in E. coli K-12.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2139644 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC208843 PMC208843]


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|{{TableHeadingColor}} |'''Abstract''' || Bacteriophage lambda missing its chromosomal attachment site will integrate into recA+ Escherichia coli K-12 and C at the sites of cryptic prophages. The specific regions in which these recombination events occur were identified in both lambda and the bacterial chromosomes. A NotI restriction site on the prophage allowed its physical mapping. This allowed us to identify the locations of Rac, Qin, and Qsr' cryptic prophages on the NotI map of E. coli K-12 and, by analogy, to identify the cryptic prophage in E. coli C as Qin. No new cryptic prophages were detected in E. coli K-12.
!align=left  |Keywords
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Bacteriophage lambda/genetics; Blotting, Southern; Chromosomes, Bacterial; Escherichia coli/genetics; Genes, Viral; Genotype; Lysogeny; Plasmids; Rec A Recombinases/genetics; Recombination, Genetic; Restriction Mapping
|{{TableHeadingColor}} nowrap |'''Links''' ||  [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2139644 PubMed]
 
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==Annotations {{HelpIcon|Help:Literature}}==
==Annotations {{HelpIcon|Help:Literature}}==
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Latest revision as of 04:38, 15 September 2013

Citation

Lichens-Park, A, Smith, CL and Syvanen, M (1990) Integration of bacteriophage lambda into the cryptic lambdoid prophages of Escherichia coli. J. Bacteriol. 172:2201-8

Abstract

Bacteriophage lambda missing its chromosomal attachment site will integrate into recA+ Escherichia coli K-12 and C at the sites of cryptic prophages. The specific regions in which these recombination events occur were identified in both lambda and the bacterial chromosomes. A NotI restriction site on the prophage allowed its physical mapping. This allowed us to identify the locations of Rac, Qin, and Qsr' cryptic prophages on the NotI map of E. coli K-12 and, by analogy, to identify the cryptic prophage in E. coli C as Qin. No new cryptic prophages were detected in E. coli K-12.

Links

PubMed PMC208843

Keywords

Bacteriophage lambda/genetics; Blotting, Southern; Chromosomes, Bacterial; Escherichia coli/genetics; Genes, Viral; Genotype; Lysogeny; Plasmids; Rec A Recombinases/genetics; Recombination, Genetic; Restriction Mapping

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