PMID:7758961: Difference between revisions

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<span style = 'font-size: large'> '''Belyaev, AS, Hails, RS and Roy, P'''  (1995) High-level expression of five foreign genes by a single recombinant baculovirus.''Gene'' '''156''':229-33</span>
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!align=left  |Citation
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'''Belyaev, AS, Hails, RS and Roy, P'''  (1995) High-level expression of five foreign genes by a single recombinant baculovirus. ''Gene'' '''156''':229-33
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!align=left  |Abstract
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Co-infection with several viruses is often used to achieve simultaneous expression of several proteins. From co-infections involving several viruses, the ratio of proteins synthesized in individual cells can be very variable. This is disadvantageous where proteins are needed to interact to provide a maximum yield of a complex product. Multiple-gene-expression vectors offer an alternative to co-infections. They enable reproducible ratios of products to be provided in each infected cell. Until recently, multigene-expression vectors have only been developed to make two proteins simultaneously. Here, we describe the generation of a single recombinant baculovirus synthesising up to five foreign proteins with a fixed ratio comparable to the ratio during the synthesis of native proteins.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7758961 PubMed]


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|{{TableHeadingColor}} |'''Abstract''' || Co-infection with several viruses is often used to achieve simultaneous expression of several proteins. From co-infections involving several viruses, the ratio of proteins synthesized in individual cells can be very variable. This is disadvantageous where proteins are needed to interact to provide a maximum yield of a complex product. Multiple-gene-expression vectors offer an alternative to co-infections. They enable reproducible ratios of products to be provided in each infected cell. Until recently, multigene-expression vectors have only been developed to make two proteins simultaneously. Here, we describe the generation of a single recombinant baculovirus synthesising up to five foreign proteins with a fixed ratio comparable to the ratio during the synthesis of native proteins.
!align=left  |Keywords
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Animals; Baculoviridae/genetics; Bluetongue virus/genetics; Cells, Cultured; Gene Expression; Genetic Vectors/genetics; Recombinant Proteins/biosynthesis; Spodoptera/cytology; Viral Proteins/biosynthesis; Viral Proteins/genetics
|{{TableHeadingColor}} nowrap |'''Links''' ||  [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7758961 PubMed]
 
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==Significance {{HelpIcon|Help:Literature}} ==
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==Annotations {{HelpIcon|Help:Literature}}==
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Latest revision as of 04:51, 15 September 2013

Citation

Belyaev, AS, Hails, RS and Roy, P (1995) High-level expression of five foreign genes by a single recombinant baculovirus. Gene 156:229-33

Abstract

Co-infection with several viruses is often used to achieve simultaneous expression of several proteins. From co-infections involving several viruses, the ratio of proteins synthesized in individual cells can be very variable. This is disadvantageous where proteins are needed to interact to provide a maximum yield of a complex product. Multiple-gene-expression vectors offer an alternative to co-infections. They enable reproducible ratios of products to be provided in each infected cell. Until recently, multigene-expression vectors have only been developed to make two proteins simultaneously. Here, we describe the generation of a single recombinant baculovirus synthesising up to five foreign proteins with a fixed ratio comparable to the ratio during the synthesis of native proteins.

Links

PubMed

Keywords

Animals; Baculoviridae/genetics; Bluetongue virus/genetics; Cells, Cultured; Gene Expression; Genetic Vectors/genetics; Recombinant Proteins/biosynthesis; Spodoptera/cytology; Viral Proteins/biosynthesis; Viral Proteins/genetics

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