PMID:8385313: Difference between revisions

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<span style = 'font-size: large'> '''Belyaev, AS and Roy, P'''  (1993) Development of baculovirus triple and quadruple expression vectors: co-expression of three or four bluetongue virus proteins and the synthesis of bluetongue virus-like particles in insect cells.''Nucleic Acids Res.'' '''21''':1219-23</span>
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'''Belyaev, AS and Roy, P'''  (1993) Development of baculovirus triple and quadruple expression vectors: co-expression of three or four bluetongue virus proteins and the synthesis of bluetongue virus-like particles in insect cells. ''Nucleic Acids Res.'' '''21''':1219-23
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Baculovirus multiple gene transfer vectors pAcAB3 and pAcAB4 have been developed to facilitate the insertion of three or four foreign genes respectively into the Autographa californica nuclear polyhedrosis virus (AcNPV) genome by a single co-transfection experiment. The pAcAB3 vector contains a polyhedrin promoter and two p10 promoters on either side of the polyhedrin promoter but in opposite orientations. The pAcAB4 vector has an additional polyhedrin promoter in opposite orientation to the first copy that is in juxtaposition to the first p10 promoter. Each of these derived vectors (pAcAB3, pAcAB4) have been used for the simultaneous expression of three or four bluetongue virus (BTV) genes respectively. When Spodoptera frugiperda cells were infected with the recombinant virus (AcBT-3/2/7/5) expressing the four major structural genes of BTV, double-capsid, virus-like particles consisting of VP2, VP3, VP5 and VP7 of BTV were assembled.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8385313 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC309285 PMC309285]


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|{{TableHeadingColor}} |'''Abstract''' || Baculovirus multiple gene transfer vectors pAcAB3 and pAcAB4 have been developed to facilitate the insertion of three or four foreign genes respectively into the Autographa californica nuclear polyhedrosis virus (AcNPV) genome by a single co-transfection experiment. The pAcAB3 vector contains a polyhedrin promoter and two p10 promoters on either side of the polyhedrin promoter but in opposite orientations. The pAcAB4 vector has an additional polyhedrin promoter in opposite orientation to the first copy that is in juxtaposition to the first p10 promoter. Each of these derived vectors (pAcAB3, pAcAB4) have been used for the simultaneous expression of three or four bluetongue virus (BTV) genes respectively. When Spodoptera frugiperda cells were infected with the recombinant virus (AcBT-3/2/7/5) expressing the four major structural genes of BTV, double-capsid, virus-like particles consisting of VP2, VP3, VP5 and VP7 of BTV were assembled.
!align=left  |Keywords
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Animals; Baculoviridae/genetics; Base Sequence; Bluetongue virus/genetics; Cell Line; Cloning, Molecular/methods; DNA, Recombinant; DNA, Viral; Electrophoresis, Polyacrylamide Gel; Genetic Vectors; Microscopy, Electron; Molecular Sequence Data; Moths; Plasmids; Promoter Regions, Genetic; Viral Structural Proteins/genetics; Viral Structural Proteins/metabolism; Virion/metabolism
|{{TableHeadingColor}} nowrap |'''Links''' ||  [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8385313 PubMed]
 
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Latest revision as of 04:54, 15 September 2013

Citation

Belyaev, AS and Roy, P (1993) Development of baculovirus triple and quadruple expression vectors: co-expression of three or four bluetongue virus proteins and the synthesis of bluetongue virus-like particles in insect cells. Nucleic Acids Res. 21:1219-23

Abstract

Baculovirus multiple gene transfer vectors pAcAB3 and pAcAB4 have been developed to facilitate the insertion of three or four foreign genes respectively into the Autographa californica nuclear polyhedrosis virus (AcNPV) genome by a single co-transfection experiment. The pAcAB3 vector contains a polyhedrin promoter and two p10 promoters on either side of the polyhedrin promoter but in opposite orientations. The pAcAB4 vector has an additional polyhedrin promoter in opposite orientation to the first copy that is in juxtaposition to the first p10 promoter. Each of these derived vectors (pAcAB3, pAcAB4) have been used for the simultaneous expression of three or four bluetongue virus (BTV) genes respectively. When Spodoptera frugiperda cells were infected with the recombinant virus (AcBT-3/2/7/5) expressing the four major structural genes of BTV, double-capsid, virus-like particles consisting of VP2, VP3, VP5 and VP7 of BTV were assembled.

Links

PubMed PMC309285

Keywords

Animals; Baculoviridae/genetics; Base Sequence; Bluetongue virus/genetics; Cell Line; Cloning, Molecular/methods; DNA, Recombinant; DNA, Viral; Electrophoresis, Polyacrylamide Gel; Genetic Vectors; Microscopy, Electron; Molecular Sequence Data; Moths; Plasmids; Promoter Regions, Genetic; Viral Structural Proteins/genetics; Viral Structural Proteins/metabolism; Virion/metabolism

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