pGEM-3Z: Difference between revisions

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==At a Glance==
==At a Glance==
[[Image:#Pgem 3z.gif|thumb|440px|right|Pgem 3z, Image provided by [http://www.promega.com/figures/popup.asp?fn=0278va&partno=P2151&product=pGEM%3Csup%3E%26%23x00AE%3B%3C%2Fsup%3E%2D3Z+Vector Promega]]]
[[File:PGEM-3Z.jpg|thumb|450px|pGEM-3Z, Image made with [[PlasMapper]]]]
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{|{{Prettytable}} width='30%' align=left
{| {{Prettytable}} width='30%' align=left id="B47252298a3ba8"  class=" tableEdit PlasmidTable" 
 
|-
|-
!{{table heading style}} |Type
!align=left {{table heading style}} |Type
|
||
Cloning
Cloning
|-
|-
!{{table heading style}} |Origin of Replication
!align=left {{table heading style}} |Origin of Replication
|
||


|-
|-
!{{table heading style}} |Copy #
!align=left {{table heading style}} |Copy #
|
||


|-
|-
!{{table heading style}} |Markers
!align=left {{table heading style}} |Markers
|
||
Ampicillin
Ampicillin
|-
|-
!{{table heading style}} |Link to Sequence
!align=left {{table heading style}} |Link to Sequence
|
||
[http://www.promega.com/vectors/pgem3z.txt Promega]
[http://www.ncbi.nlm.nih.gov/nuccore/7018282?report=fasta NCBI]
|-
|-
!{{table heading style}} |Features
!align=left {{table heading style}} |Features
|
||
SP6 RNA polymerase promoter <br>
SP6 RNA polymerase promoter <br />
T7 RNA polymerase promoter<br>
T7 RNA polymerase promoter<br />
MCS (multiple cloning site)
MCS (multiple cloning site)
|-
|-
!{{table heading style}} |Accession Number
!align=left {{table heading style}} |Accession Number
|
||
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=7018282 X65304]
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=7018282 X65304]
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==Notes==
==Notes==
The pGEM-3Z Vector is intended for use as a standard cloning vector, as well as for the synthesis of RNA in vitro. The vector carries the lacZ α-peptide and the multiple cloning region arrangement from pUC18 allowing selection of recombinants by blue/white screening.
In addition, the vector contains both the SP6 and T7 RNA polymerase promoters flanking the multiple cloning region.


==History {{HelpIcon|Help:Plasmid}}==
==History {{HelpIcon|Help:Plasmid}}==
Line 51: Line 57:


==Sources==
==Sources==
[http://www.promega.com/catalog/catalogproducts.asp?catalog_name=Promega_Products&category_name=pGEM-3Z+Vector Promega]
[http://www.promega.com/products/cloning-and-dna-markers/cloning-tools-and-competent-cells/pgem-and-psp-subcloning-vectors/pgem_3z-vector/ Promega]


==References==
==References==
{{BacktoTop}}
{{RefHelp}}
{{RefHelp}}
<references/>
<references />
 
<ref name='PMID:2985470'/>
==Sequence==
==Sequence==
  1    GGGCGAATTC GAGCTCGGTA CCCGGGGATC CTCTAGAGTC GACCTGCAGG
  1    GGGCGAATTC GAGCTCGGTA CCCGGGGATC CTCTAGAGTC GACCTGCAGG
Line 119: Line 126:




[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]][[Category:T7 Promoter]][[Category:SP6 Promoter]]
[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]][[Category:T7 Promoter]][[Category:SP6 Promoter]][[Category:MCS]]
__NOTOC__
__NOTOC__

Latest revision as of 17:00, 18 August 2011

At a Glance

pGEM-3Z, Image made with PlasMapper
Type

Cloning

Origin of Replication
Copy #
Markers

Ampicillin

Link to Sequence

NCBI

Features

SP6 RNA polymerase promoter
T7 RNA polymerase promoter
MCS (multiple cloning site)

Accession Number

X65304

edit table



Notes

The pGEM-3Z Vector is intended for use as a standard cloning vector, as well as for the synthesis of RNA in vitro. The vector carries the lacZ α-peptide and the multiple cloning region arrangement from pUC18 allowing selection of recombinants by blue/white screening.

In addition, the vector contains both the SP6 and T7 RNA polymerase promoters flanking the multiple cloning region.

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Promega

References

[back to top]


See Help:References for how to manage references in EcoliWiki.

[1]

Sequence

1     GGGCGAATTC GAGCTCGGTA CCCGGGGATC CTCTAGAGTC GACCTGCAGG
51    CATGCAAGCT TGAGTATTCT ATAGTGTCAC CTAAATAGCT TGGCGTAATC 
101   ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC
151   ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA
201   GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC
251   GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA
301   GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG
351   CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC
401   GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT
451   GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG
501   GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
551   CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT
601   TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT
651   ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA
701   TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC
751   TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC
801   GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT
851   GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG
901   CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA
951   GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT
1001  TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT
1051  TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT
1101  CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG
1151  TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC
1201  TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA
1251  ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC
1301  GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA
1351  TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA
1401  CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC
1451  AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA
1501  TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT
1551  AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG
1601  CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC
1651  GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT
1701  CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT
1751  TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT
1801  TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG
1851  CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC
1901  ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC
1951  GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC
2001  ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC
2051  TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG
2101  CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA
2151  AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT
2201  TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
2251  CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT
2301  AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG ATGACGGTGA
2351  AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG
2401  CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC
2451  GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG
2501  AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT
2551  ACCGCATCAG GCGCCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG
2601  CGATCGGTGC GGGCCTCTTC GCTATTACGC CAGCTGGCGA AAGGGGGATG
2651  TGCTGCAAGG CGATTAAGTT GGGTAACGCC AGGGTTTTCC CAGTCACGAC
2701  GTTGTAAAAC GACGGCCAGT GAATTGTAAT ACGACTCACT ATA
  1. ↑ Yanisch-Perron, C et al. (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33 103-19 PubMed
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