pACYC184: Difference between revisions
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==At a Glance | ==At a Glance== | ||
[[File:pACYC184.png|thumb|450px|pACYC184, image BCCM/LMBP]] | |||
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|- | |- | ||
!{{table heading style}} |Type | !align=left {{table heading style}} |Type | ||
| | || | ||
Cloning | Cloning | ||
|- | |- | ||
!{{table heading style}} |Origin of Replication | !align=left {{table heading style}} |Origin of Replication | ||
| | || | ||
p15A | p15A | ||
|- | |||
!align=left {{table heading style}} |Copy # | |||
|| | |||
low, 15 copies/cell | |||
|- | |- | ||
!{{table heading style}} | | !align=left {{table heading style}} |Markers | ||
| | || | ||
Chloramphenicol Tetracycline | |||
|- | |- | ||
!{{table heading style}} | | !align=left {{table heading style}} |Link to Sequence | ||
| | || | ||
[http://bccm.belspo.be/catalogues/files/lmbp-plasmids/p992.txt LMBP 992] | |||
|- | |- | ||
!{{table heading style}} | | !align=left {{table heading style}} |Features | ||
| | || | ||
|- | |- | ||
!{{table heading style}} | | !align=left {{table heading style}} |Accession Number | ||
| | || | ||
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=X06403 X06403] | |||
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==Notes== | ==Notes== | ||
This multicopy plasmid is compatible with ColE1- or pMB1-derived plasmids.<br /> | |||
pACYC184 can be amplified 10-fold in the presence of spectinomycin.<br /> | |||
The chloramphenicol resistance gene is excisable as a HaeII fragment.<br /> | |||
==History {{HelpIcon|Help:Plasmid}}== | ==History {{HelpIcon|Help:Plasmid}}== | ||
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==Sources {{HelpIcon|Help:Plasmid}}== | ==Sources {{HelpIcon|Help:Plasmid}}== | ||
[http://bccm.belspo.be/catalogues/lmbp-plasmids-plasmid-details?NUM=992 BCCM/LMBP Plasmid Collection]<br /> | |||
[http://tools.invitrogen.com/content/sfs/manuals/prsete_man.pdf Invitrogen] | |||
==References== | ==References== | ||
[http://www.ncbi.nlm.nih.gov/pubmed/149110 Chang et al., J. Bacteriol. 134 (1978), 1141-1156] | |||
==Sequence {{HelpIcon|Help:Plasmid}}== | ==Sequence {{HelpIcon|Help:Plasmid}}== | ||
1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt | |||
61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt | |||
121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga | |||
181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga | |||
241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt | |||
301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc | |||
361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat | |||
421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt | |||
481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg | |||
541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact | |||
601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa | |||
661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc | |||
721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc | |||
781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa | |||
841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc | |||
901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc | |||
961 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc | |||
1021 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac | |||
1081 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt | |||
1141 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt | |||
1201 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg | |||
1261 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt | |||
1321 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc | |||
1381 aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca | |||
1441 atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc | |||
1501 atgtttgaca gcttatcatc gataagcttt aatgcggtag tttatcacag ttaaattgct | |||
1561 aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg | |||
1621 tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg | |||
1681 atatcgtcca ttccgacagc atcgccagtc actatggcgt gctgctagcg ctatatgcgt | |||
1741 tgatgcaatt tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc | |||
1801 cagtcctgct cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac | |||
1861 ccgtcctgtg gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg | |||
1921 cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg | |||
1981 ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt | |||
2041 tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc | |||
2101 tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga ccgatgccct | |||
2161 tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg | |||
2221 cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg | |||
2281 tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg | |||
2341 tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt | |||
2401 tcggcgagaa gcaggccatt atcgccggca tggcggccga cgcgctgggc tacgtcttgc | |||
2461 tggcgttcgc gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg | |||
2521 gcatcgggat gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg | |||
2581 gacagcttca aggatcgctc gcggctctta ccagcctaac ttcgatcact ggaccgctga | |||
2641 tcgtcacggc gatttatgcc gcctcggcga gcacatggaa cgggttggca tggattgtag | |||
2701 gcgccgccct ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca | |||
2761 cctcgacctg aatggaagcc ggcggcacct cgctaacgga ttcaccactc caagaattgg | |||
2821 agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc agaacatatc | |||
2881 catcgcgtcc gccatctcca gcagccgcac gcggcgcatc tcgggcagcg ttgggtcctg | |||
2941 gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc cggctaggct ggcggggttg | |||
3001 ccttactggt tagcagaatg aatcaccgat acgcgagcga acgtgaagcg actgctgctg | |||
3061 caaaacgtct gcgacctgag caacaacatg aatggtcttc ggtttccgtg tttcgtaaag | |||
3121 tctggaaacg cggaagtccc ctacgtgctg ctgaagttgc ccgcaacaga gagtggaacc | |||
3181 aaccggtgat accacgatac tatgactgag agtcaacgcc atgagcggcc tcatttctta | |||
3241 ttctgagtta caacagtccg caccgctgtc cggtagctcc ttccggtggg cgcggggcat | |||
3301 gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc | |||
3361 ggcagcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc | |||
3421 gccctgcacc attatgttcc ggatctgcat cgcaggatgc tgctggctac cctgtggaac | |||
3481 acctacatct gtattaacga agcgctaacc gtttttatca ggctctggga ggcagaataa | |||
3541 atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg gcctcaggca | |||
3601 tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa ccagcaatag | |||
3661 acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa tttgctttcg | |||
3721 aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag gcgtttaagg | |||
3781 gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc agtactgttg | |||
3841 taattcatta agcattctgc cgacatggaa gccatcacag acggcatgat gaacctgaat | |||
3901 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg | |||
3961 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg | |||
4021 attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc | |||
4081 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta | |||
4141 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg | |||
4201 aacactatcc catatcacca gctcaccgtc tttcattgcc atacg | |||
Latest revision as of 15:11, 3 October 2016
At a Glance
| Type |
Cloning |
|---|---|
| Origin of Replication |
p15A |
| Copy # |
low, 15 copies/cell |
| Markers |
Chloramphenicol Tetracycline |
| Link to Sequence | |
| Features | |
| Accession Number | |
| edit table |
Notes
This multicopy plasmid is compatible with ColE1- or pMB1-derived plasmids.
pACYC184 can be amplified 10-fold in the presence of spectinomycin.
The chloramphenicol resistance gene is excisable as a HaeII fragment.
History
You can help EcoliWiki by writing about the history of this plasmid.
Sources
BCCM/LMBP Plasmid Collection
Invitrogen
References
Chang et al., J. Bacteriol. 134 (1978), 1141-1156
Sequence
1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc
961 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
1021 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
1081 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
1141 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
1201 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
1261 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
1321 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
1381 aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca
1441 atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc
1501 atgtttgaca gcttatcatc gataagcttt aatgcggtag tttatcacag ttaaattgct
1561 aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg
1621 tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg
1681 atatcgtcca ttccgacagc atcgccagtc actatggcgt gctgctagcg ctatatgcgt
1741 tgatgcaatt tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc
1801 cagtcctgct cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac
1861 ccgtcctgtg gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg
1921 cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg
1981 ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt
2041 tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc
2101 tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga ccgatgccct
2161 tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg
2221 cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg
2281 tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg
2341 tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt
2401 tcggcgagaa gcaggccatt atcgccggca tggcggccga cgcgctgggc tacgtcttgc
2461 tggcgttcgc gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg
2521 gcatcgggat gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg
2581 gacagcttca aggatcgctc gcggctctta ccagcctaac ttcgatcact ggaccgctga
2641 tcgtcacggc gatttatgcc gcctcggcga gcacatggaa cgggttggca tggattgtag
2701 gcgccgccct ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca
2761 cctcgacctg aatggaagcc ggcggcacct cgctaacgga ttcaccactc caagaattgg
2821 agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc agaacatatc
2881 catcgcgtcc gccatctcca gcagccgcac gcggcgcatc tcgggcagcg ttgggtcctg
2941 gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc cggctaggct ggcggggttg
3001 ccttactggt tagcagaatg aatcaccgat acgcgagcga acgtgaagcg actgctgctg
3061 caaaacgtct gcgacctgag caacaacatg aatggtcttc ggtttccgtg tttcgtaaag
3121 tctggaaacg cggaagtccc ctacgtgctg ctgaagttgc ccgcaacaga gagtggaacc
3181 aaccggtgat accacgatac tatgactgag agtcaacgcc atgagcggcc tcatttctta
3241 ttctgagtta caacagtccg caccgctgtc cggtagctcc ttccggtggg cgcggggcat
3301 gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc
3361 ggcagcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc
3421 gccctgcacc attatgttcc ggatctgcat cgcaggatgc tgctggctac cctgtggaac
3481 acctacatct gtattaacga agcgctaacc gtttttatca ggctctggga ggcagaataa
3541 atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg gcctcaggca
3601 tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa ccagcaatag
3661 acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa tttgctttcg
3721 aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag gcgtttaagg
3781 gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc agtactgttg
3841 taattcatta agcattctgc cgacatggaa gccatcacag acggcatgat gaacctgaat
3901 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg
3961 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg
4021 attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc
4081 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta
4141 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg
4201 aacactatcc catatcacca gctcaccgtc tttcattgcc atacg