pSP64: Difference between revisions

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==At a Glance ==
==At a Glance ==
[[Image:#PSP64.jpg|thumb|400px|right|pSP64, Image provided by [http://www.promega.com/figures/popup.asp?fn=0302va Promega]]]
 
[[Image:PSP64_GCK.jpg|thumb|450px|right|pSP64, Key: Red = Ampicillin marker, Magenta = SP6 promoter, Stripes = MCS]]
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{|{{Prettytable}} width='30%' align=left
{| {{Prettytable}} width='30%' align=left id="O471c1ab2cfb28"  class=" tableEdit PlasmidTable" 
 
|-
|-
!{{table heading style}} |Type
!align=left {{table heading style}} |Type
|
||
 
Cloning
|-
|-
!{{table heading style}} |Origin of Replication
!align=left {{table heading style}} |Origin of Replication
|
||


|-
|-
!{{table heading style}} |Copy #
!align=left {{table heading style}} |Copy #
|
||


|-
|-
!{{table heading style}} |Markers
!align=left {{table heading style}} |Markers
|
||
 
Ampicillin
|-
|-
!{{table heading style}} |Link to Sequence
!align=left {{table heading style}} |Link to Sequence
|
||
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=58229 PubMed]
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=58229 PubMed]
|-
|-
!{{table heading style}} |Comments
!align=left {{table heading style}} |Features
|
||
SP6 promoter<br>                   
multiple cloning sites<br>       
lacZ start codon<br>                       
lac operon<br>               
lac operator<br>                   


Hind III, Pst  I, Sal  I, Acc  I, Hinc II,Xba  I, Ava  I, BamH I, Sma  I, Sac  I, EcoR I
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!align=left {{table heading style}} |Accession Number
||
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=X65327 X65327]
 
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==Notes==
Used for cloning, in vitro transcription.


==History {{HelpIcon|Help:Plasmid}}==
==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}
{{PlasmidHistory}}


==Sources {{HelpIcon|Help:Plasmid}}==
==Sources ==
{{PlasmidSources}}
[http://www.promega.com/products/pm/discontinued-vectors/#DV14 Promega]. Discontinued.


==References==
==References==
{{BacktoTop}}
{{RefHelp}}
{{RefHelp}}
<references/>
<references />


==Sequence ==
==Sequence ==
         1 gaactcgagc agctgaagct tgaattcatc gatgatatca gatctgccgg tctccctata
         1 gaatacaagc ttgggctgca ggtcgactct agaggatccc cgggcgagct cgaattcgta
       61 gtgagtcgta ttaatttcga taagccaggt taacctgcat taatgaatcg gccaacgcgc
       61 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
       121 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
       121 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
       181 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
       181 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
       241 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
       241 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
       301 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
       301 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
       361 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
       361 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
       421 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
       421 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
       481 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag
       481 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
       541 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
       541 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
       601 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
       601 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
       661 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
       661 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
       721 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
       721 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
       781 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
       781 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
       841 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
       841 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
       901 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
       901 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
       961 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
       961 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     1021 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     1021 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     1081 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     1081 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     1141 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     1141 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     1201 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     1201 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     1261 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     1261 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     1321 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     1321 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     1381 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     1381 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     1441 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     1441 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     1501 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     1501 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     1561 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     1561 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     1621 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     1621 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     1681 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     1681 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     1741 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     1741 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     1801 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     1801 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     1861 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     1861 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     1921 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     1921 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     1981 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     1981 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     2041 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     2041 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     2101 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
     2101 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     2161 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     2161 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     2221 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     2221 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     2281 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgga
     2281 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     2341 catattgtcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2341 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     2401 atttaggtga cactata
     2401 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
    2461 gagagtgcac catatcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt
    2521 agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg
    2581 cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg
    2641 agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca
    2701 accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatctggcta
    2761 gcgatgaccc tgctgattgg ttcgctgacc atttccgggg tgcggaacgg cgttaccaga
    2821 aactcagaag gttcgtccaa ccaaaccgac tctgacggca gtttacgaga gagatgatag
    2881 ggtctgcttc agtaagccag atgctacaca attaggcttg tacatattgt cgttagaacg
    2941 cggctacaat taatacataa ccttatgtat catacacata cgatttaggt gacactata






[[Category:Plasmids]]
[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]][[Category:SP6 Promoter]]
__NOTOC__
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Latest revision as of 18:30, 22 August 2011

At a Glance

pSP64, Key: Red = Ampicillin marker, Magenta = SP6 promoter, Stripes = MCS
Type

Cloning

Origin of Replication
Copy #
Markers

Ampicillin

Link to Sequence

PubMed

Features

SP6 promoter
multiple cloning sites
lacZ start codon
lac operon
lac operator

Hind III, Pst I, Sal I, Acc I, Hinc II,Xba I, Ava I, BamH I, Sma I, Sac I, EcoR I

Accession Number

X65327

edit table



Notes

Used for cloning, in vitro transcription.

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Promega. Discontinued.

References

[back to top]


See Help:References for how to manage references in EcoliWiki.


Sequence

       1 gaatacaagc ttgggctgca ggtcgactct agaggatccc cgggcgagct cgaattcgta
      61 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     121 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     181 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     241 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     301 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     361 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     421 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     481 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     541 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     601 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     661 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     721 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     781 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     841 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     901 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     961 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    1021 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    1081 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
    1141 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
    1201 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
    1261 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
    1321 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
    1381 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
    1441 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    1501 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    1561 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    1621 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    1681 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    1741 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    1801 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    1861 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    1921 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    1981 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    2041 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    2101 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    2161 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    2221 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
    2281 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
    2341 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
    2401 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
    2461 gagagtgcac catatcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt
    2521 agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg
    2581 cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg
    2641 agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca
    2701 accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatctggcta
    2761 gcgatgaccc tgctgattgg ttcgctgacc atttccgggg tgcggaacgg cgttaccaga
    2821 aactcagaag gttcgtccaa ccaaaccgac tctgacggca gtttacgaga gagatgatag
    2881 ggtctgcttc agtaagccag atgctacaca attaggcttg tacatattgt cgttagaacg
    2941 cggctacaat taatacataa ccttatgtat catacacata cgatttaggt gacactata
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