pSP64 Poly(A): Difference between revisions

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==At a Glance==
==At a Glance==
[[File:PSP64 Poly(A).jpg|thumb|450px|pSP64 Poly(A), Image made with [[PlasMapper]]]]
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{|{{Prettytable}} width='30%' align=left
{| {{Prettytable}} width='30%' align=left id="B471e0a1806df3"  class=" tableEdit PlasmidTable" 
 
|-
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!{{table heading style}} |Type
!align=left {{table heading style}} |Type
|
||
Cloning
Cloning
|-
|-
!{{table heading style}} |Origin of Replication
!align=left {{table heading style}} |Origin of Replication
|
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|-
|-
!{{table heading style}} |Copy #
!align=left {{table heading style}} |Copy #
|
||


|-
|-
!{{table heading style}} |Markers
!align=left {{table heading style}} |Markers
|
||
Beta-lactamase
Ampicillin
|-
|-
!{{table heading style}} |Link to Sequence
!align=left {{table heading style}} |Link to Sequence
|
||
[http://www.promega.com/vectors/psp64pa.txt Promega]
[http://www.ncbi.nlm.nih.gov/nuccore/X65328  NCBI]
|-
|-
!{{table heading style}} |Comments
!align=left {{table heading style}} |Features
|
||
SP6 RNA polymerase transcription initiation site<br />


SP6 RNA polymerase promoter<br />
multiple cloningregion<br />
Poly(A)region<br />
beta-lactamase (Ampr) codingregion<br />
binding site of pUC/M13 Reverse Primer<br />
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!align=left {{table heading style}} |Accession Number
||
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=7688144 X65328]
 
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==Notes==
The pSP64 Poly(A) Vector is able to be used as a standaed cloning vector and also for in vitro transcription from the SP6 promoter.  The unique feature of the pSP64 Poly (A) Vector is the fact that is can also be used to generate poly(A)+ transcripts in vitro.  The vector contains a stretch of 30 dA:dT redsidues which are inserted between SacI and EcorRI sites.  WHen foreign DNA is cloned into any polylinker site other than EcoRI in the pSP64 vector causes linearization of the recombinant plasmid with EcoRI occurs.  This allows the use of SP6 RNA polymerase in vitro to prepare RNA copies.  These copies are of the inserted sequences that conatin a synthetic 3' "poly (A)" tail of 30 residues.
==History {{HelpIcon|Help:Plasmid}}==
==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}
{{PlasmidHistory}}


==Sources==
==Sources==
[http://www.promega.com/catalog/CatalogProducts.asp?catalog%5Fname=Promega%5FProducts&category%5Fname=pSP64+Poly%28A%29+Vector&description%5Ftext=pSP64+Poly%28A%29+Vector Promega]
[http://www.promega.com/products/cloning-and-dna-markers/cloning-tools-and-competent-cells/pgem-and-psp-subcloning-vectors/psp64-poly_a_-vector/ Promega]


==References==
==References==
{{BacktoTop}}
{{RefHelp}}
{{RefHelp}}
<references/>
<references />


==Sequence==
==Sequence==
{{PlasmidSeq}}
<pre>
1  GAATACAAGC TTGGGCTGCA GGTCGACTCT AGAGGATCCC CGGGCGAGCT
 
51  CCCAAAAAAA AAAAAAAAAA AAAAAAAAAA AAACCGAATT CGTAATCATG
 
101  TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA
 
151  CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA
 
201  GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA
 
251  AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG
 
301  CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC
 
351  GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA
 
401  ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC
 
451  AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT
 
501  TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA
 
551  AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC
 
601  CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG
 
651  GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC
 
701  TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG
 
751  CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA
 
801  ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA
 
851  GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC
 
901  AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT
 
951  TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT
 
1001  AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT
 
1051  TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT
 
1101  TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA
 
1151  GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT
 
1201  AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT
 
1251  GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC
 
1301  TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC
 
1351  TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC
 
1401  GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC
 
1451  GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA
 
1501  GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA
 
1551  GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG
 
1601  TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT
 
1651  TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC
 
1701  CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG
 
1751  GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC
 
1801  TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC
 
1851  GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT
 
1901  AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA
 
1951  ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC
 
2001  GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG
 
2051  TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC
 
2101  ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA
 
2151  TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG
 
2201  AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC
 
2251  TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT AAAAATAGGC
 
2301  GTATCACGAG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC
 
2351  CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA
 
2401  TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT
 
2451  GTCGGGGCTG GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG
 
2501  CACCATTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA
 
2551  GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC
 
2601  AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC
 
2651  CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC
 
2701  CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG
 
2751  GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCTGGC TAGCGATGAC
 
2801  CCTGCTGATT GGTTCGCTGA CCATTTCCGG GTGCGGGACG GCGTTACCAG
 
2851  AAACTCAGAA GGTTCGTCCA ACCAAACCGA CTCTGACGGC AGTTTACGAG
 
2901  AGAGATGATA GGGTCTGCTT CAGTAAGCCA GATGCTACAC AATTAGGCTT
 
2951  GTACATATTG TCGTTAGAAC GCGGCTACAA TTAATACATA ACCTTATGTA
 
3001  TCATACACAT ACGATTTAGG TGACACTATA


</pre>






[[Category:Plasmids]]
[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]]
__NOTOC__
__NOTOC__

Latest revision as of 18:51, 22 August 2011

At a Glance

pSP64 Poly(A), Image made with PlasMapper
Type

Cloning

Origin of Replication
Copy #
Markers

Ampicillin

Link to Sequence

NCBI

Features

SP6 RNA polymerase transcription initiation site

SP6 RNA polymerase promoter

multiple cloningregion

Poly(A)region

beta-lactamase (Ampr) codingregion

binding site of pUC/M13 Reverse Primer

Accession Number

X65328

edit table



Notes

The pSP64 Poly(A) Vector is able to be used as a standaed cloning vector and also for in vitro transcription from the SP6 promoter. The unique feature of the pSP64 Poly (A) Vector is the fact that is can also be used to generate poly(A)+ transcripts in vitro. The vector contains a stretch of 30 dA:dT redsidues which are inserted between SacI and EcorRI sites. WHen foreign DNA is cloned into any polylinker site other than EcoRI in the pSP64 vector causes linearization of the recombinant plasmid with EcoRI occurs. This allows the use of SP6 RNA polymerase in vitro to prepare RNA copies. These copies are of the inserted sequences that conatin a synthetic 3' "poly (A)" tail of 30 residues.

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Promega

References

[back to top]


See Help:References for how to manage references in EcoliWiki.


Sequence

1  GAATACAAGC TTGGGCTGCA GGTCGACTCT AGAGGATCCC CGGGCGAGCT

51  CCCAAAAAAA AAAAAAAAAA AAAAAAAAAA AAACCGAATT CGTAATCATG

101  TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA

151  CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA

201  GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA

251  AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG

301  CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC

351  GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA

401  ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC

451  AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT

501  TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA

551  AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC

601  CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG

651  GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC

701  TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG

751  CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA

801  ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA

851  GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC

901  AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT

951  TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT

1001  AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT

1051  TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT

1101  TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA

1151  GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT

1201  AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT

1251  GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC

1301  TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC

1351  TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC

1401  GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC

1451  GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA

1501  GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA

1551  GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG

1601  TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT

1651  TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC

1701  CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG

1751  GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC

1801  TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC

1851  GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT

1901  AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA

1951  ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC

2001  GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG

2051  TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC

2101  ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA

2151  TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG

2201  AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC

2251  TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT AAAAATAGGC

2301  GTATCACGAG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC

2351  CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA

2401  TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT

2451  GTCGGGGCTG GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG

2501  CACCATTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA

2551  GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC

2601  AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC

2651  CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC

2701  CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG

2751  GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCTGGC TAGCGATGAC

2801  CCTGCTGATT GGTTCGCTGA CCATTTCCGG GTGCGGGACG GCGTTACCAG

2851  AAACTCAGAA GGTTCGTCCA ACCAAACCGA CTCTGACGGC AGTTTACGAG

2901  AGAGATGATA GGGTCTGCTT CAGTAAGCCA GATGCTACAC AATTAGGCTT

2951  GTACATATTG TCGTTAGAAC GCGGCTACAA TTAATACATA ACCTTATGTA

3001  TCATACACAT ACGATTTAGG TGACACTATA
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