pGL2-Enhancer: Difference between revisions

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==At a Glance==
==At a Glance==
[[Image:pGL2-Enhancer.gif|thumb|right|350px|pGL2-Enhancer, image provided by [http://www.promega.com/figures/popup.asp?fn=0314va Promega]]]
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{|{{Prettytable}} width='30%' align=left
{| {{Prettytable}} width='30%' align=left id="Z47176b3de3da6"  class=" tableEdit PlasmidTable" 
 
|-
|-
!{{table heading style}} |Type
!align=left {{table heading style}} |Type
|
||
Cloning
Cloning
|-
|-
!{{table heading style}} |Origin of Replication
!align=left {{table heading style}} |Origin of Replication
|
||
ColE1
f1
|-
|-
!{{table heading style}} |Copy #
!align=left {{table heading style}} |Copy #
|
||


|-
|-
!{{table heading style}} |Markers
!align=left {{table heading style}} |Markers
|
||
Beta-lactamase
Ampicillin
|-
|-
!{{table heading style}} |Link to Sequence
!align=left {{table heading style}} |Link to Sequence
|
||
[http://www.promega.com/vectors/pgl2-e.txt Promega]
[http://www.ncbi.nlm.nih.gov/nuccore/27650341?report=fasta NCBI]
|-
|-
!{{table heading style}} |Comments
!align=left {{table heading style}} |Features
|
||
Contains an SV40 enhancer derived from the SV40 early promoter, located downstream of the luc gene after the poly(A) signal
Multiple cloning region<Br />
Luciferase gene (luc)<Br />
GLprimer2 binding site<Br />
SV40 late poly(A) signal<Br />
SV40 Enhancer<Br />
RVprimer4 binding site<Br />
ColE1-derived plasmid replication origin<Br />
lactamase (Ampr) gene<Br />
GLprimer1 binding site<Br />
|-
|-
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!align=left {{table heading style}} |Accession Number
||
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=27650341 X65325]
 
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==Notes==
The pGL2-Enhancer Vector contains an SV40 enhancer, derived from the SV40 early promoter, located downstream of the luciferase gene after the poly(A) signal. This aids in the verification of functional promoter elements because the presence of the enhancer in many cases will result in transcription of luc at higher levels.
==History {{HelpIcon|Help:Plasmid}}==
==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}
{{PlasmidHistory}}


==Sources==
==Sources==
{{PlasmidSources}}
[http://www.promega.com/search-results/?q=pGL2-Enhancer Promega]


==References==
==References==
{{BacktoTop}}
{{RefHelp}}
{{RefHelp}}
<references/>
<references />
 
<ref name='PMID:2889129'/>
<ref name='PMID:3323819'/>
==Sequence==
==Sequence==
{{PlasmidSeq}}
<pre>
 
    1  CCCGGGAGGT ACCGAGCTCT TACGCGTGCT AGCTCGAGAT CTAAGTAAGC
  51  TTGGCATTCC GGTACTGTTG GTAAAATGGA AGACGCCAAA AACATAAAGA
  101  AAGGCCCGGC GCCATTCTAT CCTCTAGAGG ATGGAACCGC TGGAGAGCAA
  151  CTGCATAAGG CTATGAAGAG ATACGCCCTG GTTCCTGGAA CAATTGCTTT
  201  TACAGATGCA CATATCGAGG TGAACATCAC GTACGCGGAA TACTTCGAAA
  251  TGTCCGTTCG GTTGGCAGAA GCTATGAAAC GATATGGGCT GAATACAAAT
  301  CACAGAATCG TCGTATGCAG TGAAAACTCT CTTCAATTCT TTATGCCGGT
  351  GTTGGGCGCG TTATTTATCG GAGTTGCAGT TGCGCCCGCG AACGACATTT
  401  ATAATGAACG TGAATTGCTC AACAGTATGA ACATTTCGCA GCCTACCGTA
  451  GTGTTTGTTT CCAAAAAGGG GTTGCAAAAA ATTTTGAACG TGCAAAAAAA
  501  ATTACCAATA ATCCAGAAAA TTATTATCAT GGATTCTAAA ACGGATTACC
  551  AGGGATTTCA GTCGATGTAC ACGTTCGTCA CATCTCATCT ACCTCCCGGT
  601  TTTAATGAAT ACGATTTTGT ACCAGAGTCC TTTGATCGTG ACAAAACAAT
  651  TGCACTGATA ATGAATTCCT CTGGATCTAC TGGGTTACCT AAGGGTGTGG
  701  CCCTTCCGCA TAGAACTGCC TGCGTCAGAT TCTCGCATGC CAGAGATCCT
  751  ATTTTTGGCA ATCAAATCAT TCCGGATACT GCGATTTTAA GTGTTGTTCC
  801  ATTCCATCAC GGTTTTGGAA TGTTTACTAC ACTCGGATAT TTGATATGTG
  851  GATTTCGAGT CGTCTTAATG TATAGATTTG AAGAAGAGCT GTTTTTACGA
  901  TCCCTTCAGG ATTACAAAAT TCAAAGTGCG TTGCTAGTAC CAACCCTATT
  951  TTCATTCTTC GCCAAAAGCA CTCTGATTGA CAAATACGAT TTATCTAATT
1001  TACACGAAAT TGCTTCTGGG GGCGCACCTC TTTCGAAAGA AGTCGGGGAA
1051  GCGGTTGCAA AACGCTTCCA TCTTCCAGGG ATACGACAAG GATATGGGCT
1101  CACTGAGACT ACATCAGCTA TTCTGATTAC ACCCGAGGGG GATGATAAAC
1151  CGGGCGCGGT CGGTAAAGTT GTTCCATTTT TTGAAGCGAA GGTTGTGGAT
1201  CTGGATACCG GGAAAACGCT GGGCGTTAAT CAGAGAGGCG AATTATGTGT
1251  CAGAGGACCT ATGATTATGT CCGGTTATGT AAACAATCCG GAAGCGACCA
1301  ACGCCTTGAT TGACAAGGAT GGATGGCTAC ATTCTGGAGA CATAGCTTAC
1351  TGGGACGAAG ACGAACACTT CTTCATAGTT GACCGCTTGA AGTCTTTAAT
1401  TAAATACAAA GGATATCAGG TGGCCCCCGC TGAATTGGAA TCGATATTGT
1451  TACAACACCC CAACATCTTC GACGCGGGCG TGGCAGGTCT TCCCGACGAT
1501  GACGCCGGTG AACTTCCCGC CGCCGTTGTT GTTTTGGAGC ACGGAAAGAC
1551  GATGACGGAA AAAGAGATCG TGGATTACGT CGCCAGTCAA GTAACAACCG
1601  CGAAAAAGTT GCGCGGAGGA GTTGTGTTTG TGGACGAAGT ACCGAAAGGT
1651  CTTACCGGAA AACTCGACGC AAGAAAAATC AGAGAGATCC TCATAAAGGC
1701  CAAGAAGGGC GGAAAGTCCA AATTGTAAAA TGTAACTGTA TTCAGCGATG
1751  ACGAAATTCT TAGCTATTGT AATACTGCGA TGAGTGGCAG GGCGGGGCGT
1801  AATTTTTTTA AGGCAGTTAT TGGTGCCCTT AAACGCCTGG TTGCTACGCC
1851  TGAATAAGTG ATAATAAGCG GATGAATGGC AGAAATTCGC CGGATCTTTG
1901  TGAAGGAACC TTACTTCTGT GGTGTGACAT AATTGGACAA ACTACCTACA
1951  GAGATTTAAA GCTCTAAGGT AAATATAAAA TTTTTAAGTG TATAATGTGT
2001  TAAACTACTG ATTCTAATTG TTTGTGTATT TTAGATTCCA ACCTATGGAA
2051  CTGATGAATG GGAGCAGTGG TGGAATGCCT TTAATGAGGA AAACCTGTTT
2101  TGCTCAGAAG AAATGCCATC TAGTGATGAT GAGGCTACTG CTGACTCTCA
2151  ACATTCTACT CCTCCAAAAA AGAAGAGAAA GGTAGAAGAC CCCAAGGACT
2201  TTCCTTCAGA ATTGCTAAGT TTTTTGAGTC ATGCTGTGTT TAGTAATAGA
2251  ACTCTTGCTT GCTTTGCTAT TTACACCACA AAGGAAAAAG CTGCACTGCT
2301  ATACAAGAAA ATTATGGAAA AATATTCTGT AACCTTTATA AGTAGGCATA
2351  ACAGTTATAA TCATAACATA CTGTTTTTTC TTACTCCACA CAGGCATAGA
2401  GTGTCTGCTA TTAATAACTA TGCTCAAAAA TTGTGTACCT TTAGCTTTTT
2451  AATTTGTAAA GGGGTTAATA AGGAATATTT GATGTATAGT GCCTTGACTA
2501  GAGATCATAA TCAGCCATAC CACATTTGTA GAGGTTTTAC TTGCTTTAAA
2551  AAACCTCCCA CACCTCCCCC TGAACCTGAA ACATAAAATG AATGCAATTG
2601  TTGTTGTTAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT
2651  AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG
2701  TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGG ATCTGAACCA
2751  TGGAGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG
2801  AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA
2851  TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC
2901  TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA
2951  CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCAGATCC TCTAGGGATC
3001  CGTCGACCGA TGCCCTTGAG AGCCTTCAAC CCAGTCAGCT CCTTCCGGTG
3051  GGCGCGGGGC ATGACTATCG TCGCCGCACT TATGACTGTC TTCTTTATCA
3101  TGCAACTCGT AGGACAGGTG CCGGCAGCGC TCTTCCGCTT CCTCGCTCAC
3151  TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT
3201  CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG
3251  AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC
3301  GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA
3351  ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC
3401  CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT
3451  GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
3501  TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC
3551  TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC
3601  CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT
3651  CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA
3701  GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG
3751  AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
3801  AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT
3851  GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
3901  AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA
3951  ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC
4001  TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA
4051  TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA
4101  TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC
4151  GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
4201  AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA
4251  ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC
4301  GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC
4351  GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG
4401  TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA
4451  TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
4501  CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC
4551  TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA
4601  AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA
4651  GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA
4701  CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT
4751  TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
4801  GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA
4851  GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA
4901  ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA
4951  TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
5001  AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA
5051  AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT
5101  GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG
5151  CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC
5201  CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT
5251  ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG
5301  GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG
5351  TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT
5401  CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT
5451  GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTTAACAAA
5501  ATATTAACGC TTACAATTTG CCATTCGCCA TTCAGGCTGC GCAACTGTTG
5551  GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT ATTACGCCAG CCCAAGCTAC
5601  CATGATAAGT AAGTAATATT AAGGTACGTG GAGGTTTTAC TTGCTTTAAA
5651  AAACCTCCCA CACCTCCCCC TGAACCTGAA ACATAAAATG AATGCAATTG
5701  TTGTTGTTAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT
5751  AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG
5801  TGGTTTGTCC AAACTCATCA ATGTATCTTA TGGTACTGTA ACTGAGCTAA
5851  CATAA


</pre>




[[Category:Plasmids]]
[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]]
__NOTOC__
__NOTOC__

Latest revision as of 21:50, 29 August 2011

At a Glance

pGL2-Enhancer, image provided by Promega
Type

Cloning

Origin of Replication

f1

Copy #
Markers

Ampicillin

Link to Sequence

NCBI

Features

Multiple cloning region
Luciferase gene (luc)
GLprimer2 binding site
SV40 late poly(A) signal
SV40 Enhancer
RVprimer4 binding site
ColE1-derived plasmid replication origin
lactamase (Ampr) gene
GLprimer1 binding site

Accession Number

X65325

edit table



Notes

The pGL2-Enhancer Vector contains an SV40 enhancer, derived from the SV40 early promoter, located downstream of the luciferase gene after the poly(A) signal. This aids in the verification of functional promoter elements because the presence of the enhancer in many cases will result in transcription of luc at higher levels.

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Promega

References

[back to top]


See Help:References for how to manage references in EcoliWiki.

[1] [2]

Sequence

    1  CCCGGGAGGT ACCGAGCTCT TACGCGTGCT AGCTCGAGAT CTAAGTAAGC
   51  TTGGCATTCC GGTACTGTTG GTAAAATGGA AGACGCCAAA AACATAAAGA
  101  AAGGCCCGGC GCCATTCTAT CCTCTAGAGG ATGGAACCGC TGGAGAGCAA
  151  CTGCATAAGG CTATGAAGAG ATACGCCCTG GTTCCTGGAA CAATTGCTTT
  201  TACAGATGCA CATATCGAGG TGAACATCAC GTACGCGGAA TACTTCGAAA
  251  TGTCCGTTCG GTTGGCAGAA GCTATGAAAC GATATGGGCT GAATACAAAT
  301  CACAGAATCG TCGTATGCAG TGAAAACTCT CTTCAATTCT TTATGCCGGT
  351  GTTGGGCGCG TTATTTATCG GAGTTGCAGT TGCGCCCGCG AACGACATTT
  401  ATAATGAACG TGAATTGCTC AACAGTATGA ACATTTCGCA GCCTACCGTA
  451  GTGTTTGTTT CCAAAAAGGG GTTGCAAAAA ATTTTGAACG TGCAAAAAAA
  501  ATTACCAATA ATCCAGAAAA TTATTATCAT GGATTCTAAA ACGGATTACC
  551  AGGGATTTCA GTCGATGTAC ACGTTCGTCA CATCTCATCT ACCTCCCGGT
  601  TTTAATGAAT ACGATTTTGT ACCAGAGTCC TTTGATCGTG ACAAAACAAT
  651  TGCACTGATA ATGAATTCCT CTGGATCTAC TGGGTTACCT AAGGGTGTGG
  701  CCCTTCCGCA TAGAACTGCC TGCGTCAGAT TCTCGCATGC CAGAGATCCT
  751  ATTTTTGGCA ATCAAATCAT TCCGGATACT GCGATTTTAA GTGTTGTTCC
  801  ATTCCATCAC GGTTTTGGAA TGTTTACTAC ACTCGGATAT TTGATATGTG
  851  GATTTCGAGT CGTCTTAATG TATAGATTTG AAGAAGAGCT GTTTTTACGA
  901  TCCCTTCAGG ATTACAAAAT TCAAAGTGCG TTGCTAGTAC CAACCCTATT
  951  TTCATTCTTC GCCAAAAGCA CTCTGATTGA CAAATACGAT TTATCTAATT
 1001  TACACGAAAT TGCTTCTGGG GGCGCACCTC TTTCGAAAGA AGTCGGGGAA
 1051  GCGGTTGCAA AACGCTTCCA TCTTCCAGGG ATACGACAAG GATATGGGCT
 1101  CACTGAGACT ACATCAGCTA TTCTGATTAC ACCCGAGGGG GATGATAAAC
 1151  CGGGCGCGGT CGGTAAAGTT GTTCCATTTT TTGAAGCGAA GGTTGTGGAT
 1201  CTGGATACCG GGAAAACGCT GGGCGTTAAT CAGAGAGGCG AATTATGTGT
 1251  CAGAGGACCT ATGATTATGT CCGGTTATGT AAACAATCCG GAAGCGACCA
 1301  ACGCCTTGAT TGACAAGGAT GGATGGCTAC ATTCTGGAGA CATAGCTTAC
 1351  TGGGACGAAG ACGAACACTT CTTCATAGTT GACCGCTTGA AGTCTTTAAT
 1401  TAAATACAAA GGATATCAGG TGGCCCCCGC TGAATTGGAA TCGATATTGT
 1451  TACAACACCC CAACATCTTC GACGCGGGCG TGGCAGGTCT TCCCGACGAT
 1501  GACGCCGGTG AACTTCCCGC CGCCGTTGTT GTTTTGGAGC ACGGAAAGAC
 1551  GATGACGGAA AAAGAGATCG TGGATTACGT CGCCAGTCAA GTAACAACCG
 1601  CGAAAAAGTT GCGCGGAGGA GTTGTGTTTG TGGACGAAGT ACCGAAAGGT
 1651  CTTACCGGAA AACTCGACGC AAGAAAAATC AGAGAGATCC TCATAAAGGC
 1701  CAAGAAGGGC GGAAAGTCCA AATTGTAAAA TGTAACTGTA TTCAGCGATG
 1751  ACGAAATTCT TAGCTATTGT AATACTGCGA TGAGTGGCAG GGCGGGGCGT
 1801  AATTTTTTTA AGGCAGTTAT TGGTGCCCTT AAACGCCTGG TTGCTACGCC
 1851  TGAATAAGTG ATAATAAGCG GATGAATGGC AGAAATTCGC CGGATCTTTG
 1901  TGAAGGAACC TTACTTCTGT GGTGTGACAT AATTGGACAA ACTACCTACA
 1951  GAGATTTAAA GCTCTAAGGT AAATATAAAA TTTTTAAGTG TATAATGTGT
 2001  TAAACTACTG ATTCTAATTG TTTGTGTATT TTAGATTCCA ACCTATGGAA
 2051  CTGATGAATG GGAGCAGTGG TGGAATGCCT TTAATGAGGA AAACCTGTTT
 2101  TGCTCAGAAG AAATGCCATC TAGTGATGAT GAGGCTACTG CTGACTCTCA
 2151  ACATTCTACT CCTCCAAAAA AGAAGAGAAA GGTAGAAGAC CCCAAGGACT
 2201  TTCCTTCAGA ATTGCTAAGT TTTTTGAGTC ATGCTGTGTT TAGTAATAGA
 2251  ACTCTTGCTT GCTTTGCTAT TTACACCACA AAGGAAAAAG CTGCACTGCT
 2301  ATACAAGAAA ATTATGGAAA AATATTCTGT AACCTTTATA AGTAGGCATA
 2351  ACAGTTATAA TCATAACATA CTGTTTTTTC TTACTCCACA CAGGCATAGA
 2401  GTGTCTGCTA TTAATAACTA TGCTCAAAAA TTGTGTACCT TTAGCTTTTT
 2451  AATTTGTAAA GGGGTTAATA AGGAATATTT GATGTATAGT GCCTTGACTA
 2501  GAGATCATAA TCAGCCATAC CACATTTGTA GAGGTTTTAC TTGCTTTAAA
 2551  AAACCTCCCA CACCTCCCCC TGAACCTGAA ACATAAAATG AATGCAATTG
 2601  TTGTTGTTAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT
 2651  AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG
 2701  TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGG ATCTGAACCA
 2751  TGGAGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG
 2801  AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA
 2851  TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC
 2901  TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA
 2951  CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCAGATCC TCTAGGGATC
 3001  CGTCGACCGA TGCCCTTGAG AGCCTTCAAC CCAGTCAGCT CCTTCCGGTG
 3051  GGCGCGGGGC ATGACTATCG TCGCCGCACT TATGACTGTC TTCTTTATCA
 3101  TGCAACTCGT AGGACAGGTG CCGGCAGCGC TCTTCCGCTT CCTCGCTCAC
 3151  TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT
 3201  CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG
 3251  AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC
 3301  GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA
 3351  ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC
 3401  CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT
 3451  GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
 3501  TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC
 3551  TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC
 3601  CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT
 3651  CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA
 3701  GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG
 3751  AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
 3801  AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT
 3851  GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
 3901  AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA
 3951  ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC
 4001  TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA
 4051  TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA
 4101  TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC
 4151  GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
 4201  AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA
 4251  ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC
 4301  GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC
 4351  GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG
 4401  TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA
 4451  TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
 4501  CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC
 4551  TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA
 4601  AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA
 4651  GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA
 4701  CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT
 4751  TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
 4801  GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA
 4851  GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA
 4901  ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA
 4951  TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
 5001  AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA
 5051  AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT
 5101  GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG
 5151  CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC
 5201  CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT
 5251  ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG
 5301  GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG
 5351  TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT
 5401  CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT
 5451  GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTTAACAAA
 5501  ATATTAACGC TTACAATTTG CCATTCGCCA TTCAGGCTGC GCAACTGTTG
 5551  GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT ATTACGCCAG CCCAAGCTAC
 5601  CATGATAAGT AAGTAATATT AAGGTACGTG GAGGTTTTAC TTGCTTTAAA
 5651  AAACCTCCCA CACCTCCCCC TGAACCTGAA ACATAAAATG AATGCAATTG
 5701  TTGTTGTTAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT
 5751  AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG
 5801  TGGTTTGTCC AAACTCATCA ATGTATCTTA TGGTACTGTA ACTGAGCTAA
 5851  CATAA
  1. ↑ Rosenthal, N (1987) Identification of regulatory elements of cloned genes with functional assays. Meth. Enzymol. 152 704-20 PubMed
  2. ↑ Henikoff, S (1987) Unidirectional digestion with exonuclease III in DNA sequence analysis. Meth. Enzymol. 155 156-65 PubMed
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