pBAD/gIII Calmodulin: Difference between revisions

Latest revision as of 16:35, 23 August 2011

At a Glance

Type	Expression
Origin of Replication	pBR322
Copy #
Markers	Ampicillin
Link to Sequence	Invitrogen
Features	araBAD Promoter Calmodulin ORF myc epitope tag 6X His Tag AraC (complementary strand)
Accession Number
edit table

Notes

The araBAD/gIII promoter of the pBAD/gIII vector provides tight regulation of secreted expression within E. coli. The fd bacteriophage fd gene III of this araBAD vector encodes for a leader peptide that directs the recombinant protein to the periplasmic space for simple purification, and its C-terminal (6xHis) & myc epitope tags enable an efficient means of purification, detection and analysis.

History

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Sources

Invitrogen

References

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Sequence

 AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCA
 AACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACA
 AAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAG
 CATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACC
 CGTTTTTTGGGCTAACAGGAGGAATTAACCATGAAAAAACTGCTGTTCGCGATTCCGCTGGTGGTGCCGTTC
 TATAGCCATAGCACCATGGAGCTCATGGCTGACCAGCTGACCGAAGAACAGATTGCAGAGTTCAAGGAAGCC
 TTCTCCCTCTTTGACAAGGATGGAGATGGCACCATTACCACCAAGGAGCTGGGGACTGTGATGAGATCGCTG
 GGGCAAAACCCCACTGAGGCGGAACTGCAGGACATGATCAATGAGGTGGATGCTGATGGCAATGGGACCATT
 GACTTCCCAGAGTTCCTGACCATGATGGCCAGAAAGATGAAGGATACAGACAGCGAGGAGGAGATACCAGAG
 GCCTTCCGTGTCTTTGACAAGGATGGGAATGGCTACATCAGTGCTGCTGAGCTGCGTCACGTCATGACGAAC
 CTGGGGGAGAAGCTGACTGATGAGGAAGTGGATGAGATGATCCGAGAGGCGGACATTGATGGAGGCGGCCAG
 GTCAATTATGAAGAGTTTGTACAGATGATGACTGCGAGGGTTCTAGAACAAAAACTCATCTCAGAAGAGGAT
 CTGAATAGCGCCGTCGACCATCATCATCATCATCATTGAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCG
 GATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGC
 CTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATG
 GTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAA
 GACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCG
 GATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAA
 ATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTTGTTTATTTTTCTAAA
 TACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGA
 GTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTC
 ACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGG
 ATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAG
 TTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATT
 CTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAAT
 TATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGA
 AGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGA
 ATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTAT
 TAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAG
 GACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGT
 CTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGA
 GTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAAC
 TGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGG
 TGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACC
 CCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAA
 AACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCT
 TCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTG
 TAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTC
 TTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCA
 CACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCA
 CGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGG
 AGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGAT
 TTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGG
 CCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCG
 CCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGG
 AAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCA
 GTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGC
 TGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAG
 ACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCA
 GCAGATCAATTCGCGCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCAAATGGACGAAGCAGGGATTCT
 GCAAACCCTATGCTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAACTTGACGGCTAC
 ATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCG
 CGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAA
 AAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAA
 AAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGC
 CAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAAT
 CGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCC
 TTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAA
 CCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCA
 CTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAACAGCAAAAT
 ATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTGACCGCGAATGGTGAGATTGAG
 AATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTCAATCGGCGTTAA
 ACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCTTCAGCCATACTTTT
 CATACTCCCGCCATTCAGAG

@@ Line 1: / Line 1: @@
 ==At a Glance==
-[[Image:#Pbadcalmodium.jpg|thumb|440px|right|pBAD/gIII Calmodulin, Image provided by [https://www.invitrogen.com/content/sfs/vectors/pbadgiiicalmodulin_map.pdf Invitrogen]]]
+[[File:PBAD-gIII Calmodulin.jpg|thumb|450px|PBAD/gIII Calmodulin, Image made with [[PlasMapper]]]]
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-{|{{Prettytable}} width='30%' align=left
+{| {{Prettytable}} width='30%' align=left  id="O4705b4dce1dbb"  class=" tableEdit PlasmidTable"
 |-
-!{{table heading style}} |Type
+!align=left {{table heading style}} |Type
-|
+||
 Expression
 |-
-!{{table heading style}} |Origin of Replication
+!align=left {{table heading style}} |Origin of Replication
-|
+||
 pBR322
 |-
-!{{table heading style}} |Copy #
+!align=left {{table heading style}} |Copy #
-|
+||
 |-
-!{{table heading style}} |Markers
+!align=left {{table heading style}} |Markers
-|
+||
 Ampicillin
 |-
-!{{table heading style}} |Link to Sequence
+!align=left {{table heading style}} |Link to Sequence
-|
+||
 [https://www.invitrogen.com/content/sfs/vectors/pbadgiiicalmod_seq.txt Invitrogen]
 |-
-!{{table heading style}} |Comments
+!align=left {{table heading style}} |Features
-|
+||
+araBAD Promoter <br />
+Calmodulin ORF <br />
+myc epitope tag <br />
+X His Tag <br />
+AraC (complementary strand) <br />
+|-
+!align=left {{table heading style}} |Accession Number
+||
-|-
-|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.O4705b4dce1dbb&page=0&pagename={{FULLPAGENAMEE}} edit table]
+|- class="tableEdit_footer"
+|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.O4705b4dce1dbb&page=112830&pagename={{FULLPAGENAMEE}}&type=1&template=PlasmidTable edit table]</span> ||
 |}
 <!--box uid=63ce1649aab8b3180235b0af7618f667.0.O4705b4dce1dbb--></protect>
 <BR>
 <Br clear='all'>
+==Notes==
+The araBAD/gIII promoter of the pBAD/gIII vector provides tight regulation of secreted expression within E. coli. The fd bacteriophage fd gene III of this araBAD vector encodes for a leader peptide that directs the recombinant protein to the periplasmic space for simple purification, and its C-terminal (6xHis) & myc epitope tags enable an efficient means of purification, detection and analysis.
 ==History {{HelpIcon|Help:Plasmid}}==
@@ Line 47: / Line 60: @@
 ==References==
+{{BacktoTop}}
 {{RefHelp}}
-<references/>
+<references />
 ==Sequence==
@@ Line 119: / Line 133: @@
-[[Category:Plasmids]][[Category:Expression Plasmids]][[Category:Invitrogen Plasmids]][[Category:Ampicillin Marker Plasmids]]
+[[Category:Plasmids]][[Category:Expression Plasmids]][[Category:Invitrogen Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:araBAD Promoter]][[Category:Calmodulin]][[Category:6X His Tag]][[Category:araC]]
 __NOTOC__