pUC19: Difference between revisions

From EcoliWiki
Jump to navigation Jump to search
← Older edit
m Reverted edits by Gwen (Talk); changed back to last version by Miriam
Joncrz (talk | contribs)
2,390 edits
Table edited by Joncrz via TableEdit
 
(22 intermediate revisions by 7 users not shown)
Line 1: Line 1:
==At a Glance==
==At a Glance==
[[Image:PUC19.jpg|thumb|500px|right||pUC19. In red is the '''bla''' gene, with lacZα in yellow and the origin of replication in green]]
<protect><!--box uid=63ce1649aab8b3180235b0af7618f667.0.A46ca2cc5e80ae-->
<protect><!--box uid=63ce1649aab8b3180235b0af7618f667.0.A46ca2cc5e80ae-->
<!--
<!--
******************************************************************************************
******************************************************************************************
*
*  
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **  
*
*  
****************************************************************************************** -->
****************************************************************************************** -->
{|{{Prettytable}} width='30%' align=left
{| {{Prettytable}} width='30%' align=left id="A46ca2cc5e80ae"  class=" tableEdit PlasmidTable" 
 
|-
|-
!{{table heading style}} |Type
!align=left {{table heading style}} |Type
|
||
Cloning
Cloning
||
|-
|-
!{{table heading style}} |Origin of Replication
!align=left {{table heading style}} |Origin of Replication
|
||
pMB1 (mutant)
pMB1 (mutant)
||
ColE1
|-
|-
!{{table heading style}} |Copy #
!align=left {{table heading style}} |Copy #
|
||
Temperature dependent<BR>
Temperature dependent
75 at 37<BR>
 
>200 at 42
75 at 37
 
200 at 42
||
High(100+)
|-
|-
!{{table heading style}} |Markers
!align=left {{table heading style}} |Markers
|
||
Ampicillin
Ampicillin
||


|-
|-
!{{table heading style}} |Link to Sequence
!align=left {{table heading style}} |Link to Sequence
|
||
[http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/puc19.txt NEB]
[http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/puc19.txt NEB]
[http://www.ncbi.nlm.nih.gov/nuccore/L09137 NCBI]
||
|-
|-
!{{table heading style}} |Comments
!align=left {{table heading style}} |Features
|
||
Multiple Cloning Site is the inverse MCS of pUC18
Multiple Cloning Site is the inverse MCS of pUC18 <ref name='PMID:2985470'/>
 
||
lacZα
MCS
|-
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.A46ca2cc5e80ae&page=0&pagename={{FULLPAGENAMEE}} edit table]
!align=left {{table heading style}} |Accession Number
||
 
||
[http://www.ncbi.nlm.nih.gov/nuccore/L09137  L09137] <br />
 
Sigma-Aldrich:M77789
 
 
 
|- class="tableEdit_footer"
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.A46ca2cc5e80ae&page=112267&pagename={{FULLPAGENAMEE}}&type=1&template=PlasmidTable edit table]</span> || ||
|}
|}
<!--box uid=63ce1649aab8b3180235b0af7618f667.0.A46ca2cc5e80ae--></protect>
<!--box uid=63ce1649aab8b3180235b0af7618f667.0.A46ca2cc5e80ae--></protect>
<BR>
<BR>
<Br clear='all'>
<Br clear='all'>
==Notes==


==History {{HelpIcon|Help:Plasmid}}==
==History {{HelpIcon|Help:Plasmid}}==
Line 46: Line 79:


==Sources==
==Sources==
[http://www.neb.com/nebecomm/products/productN3041.asp New England Biolabs]
[http://www.neb.com/nebecomm/products/productN3041.asp New England Biolabs]<br>
[http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/D3404 Sigma Aldrich]


==References==
{{BacktoTop}}
{{RefHelp}}
<references />
<ref name='PMID:2985470 '/>
==Sequence==
==Sequence==


Line 78: Line 117:
     2601  CCGAAAAGTG CCACCTGACG TCTAAGAAAC CATTATTATC ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTC
     2601  CCGAAAAGTG CCACCTGACG TCTAAGAAAC CATTATTATC ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTC


==Images==
 
[[Image:PUC19.jpg|thumb|600px|center||pUC19. In red is the '''bla''' gene, with lacZα in yellow and the origin of replication in green]]
<BR>


<BR clear='all'>
<BR clear='all'>
==References==
<ref name='PMID:2985470 '/>
<references/>


__NOTOC__
__NOTOC__




[[Category:Plasmids]][[Category:Cloning Plasmids]]
[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:NEB Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Sigma-Aldrich Plasmids]][[Category:MCS]]

Latest revision as of 22:27, 29 August 2011

At a Glance

pUC19. In red is the bla gene, with lacZα in yellow and the origin of replication in green
Type

Cloning

Origin of Replication

pMB1 (mutant)

ColE1

Copy #

Temperature dependent

75 at 37

200 at 42

High(100+)

Markers

Ampicillin

Link to Sequence

NEB

NCBI

Features

Multiple Cloning Site is the inverse MCS of pUC18 [1]

lacZα MCS

Accession Number

L09137

Sigma-Aldrich:M77789


edit table



Notes

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

New England Biolabs
Sigma Aldrich

References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. ↑ Yanisch-Perron, C et al. (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33 103-19 PubMed

[1]

Sequence

      1  TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG 
    101  TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA 
    201  CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT 
    301  TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT 
    401  CGAGCTCGGT ACCCGGGGAT CCTCTAGAGT CGACCTGCAG GCATGCAAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT 
    501  CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC 
    601  GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC 
    701  TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
    801  AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC 
    901  AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA 
   1001  CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
   1101  TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC 
   1201  TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA 
   1301  CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
   1401  CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC 
   1501  GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA 
   1601  TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC 
   1701  CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA 
   1801  ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA 
   1901  GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA 
   2001  ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA 
   2101  TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG 
   2201  AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG 
   2301  TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC 
   2401  ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC 
   2501  AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC 
   2601  CCGAAAAGTG CCACCTGACG TCTAAGAAAC CATTATTATC ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTC



  1. ↑ Yanisch-Perron, C et al. (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33 103-19 PubMed
Retrieved from "https://ecoliwiki.org/colipedia/index.php?title=pUC19&oldid=1322152"