pBEn-SET2a: Difference between revisions

Latest revision as of 16:43, 16 September 2011

At a Glance

pBEn-SET2a Image provided by Agilent-Genomics

Type	Expression
Origin of Replication	pBR322
Copy #
Markers	Ampicillin
Link to Sequence	Agilent-Genomics
Features	T7 Promoter lac Operator extended RBS SET2 tag MCS lacI repressor ORF
Accession Number
edit table

Notes

Use pBEn vectors when want to use VariFlex tags at N-terminus of protein of interest.

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Agilent-Genomics

References

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See Help:References for how to manage references in EcoliWiki.

Sequence

   1  TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA  
  51  GAAATAATTT TGTTTAACTT TAAGAAGGAG ATATACATAT GGACCCCGAA  
 101  GAGGCGAGTG TTACTTCAAC AGAAGAAACC TTAACGCCAG CACAGGAGGC  
 151  CGCAGAGACC GAAGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGAGG  
 201  CTGAAACCGC TGAGCAACTG CTGGTTCCGC GTGGATCTGG ATCCGAATTC  
 251  TCTTCCCGGG TCTTGTTCCA TGGGTCGACT CGAGCTCAAG CTTAGATCCG  
 301  GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA  
 351  GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT  
 401  TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCCGCAAG AGGCCCGGCA  
 451  GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG  
 501  AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG  
 551  TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTAA TCGAAGATAA  
 601  GCTGTCAAAC ATGACAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT  
 651  ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG  
 701  GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA  
 751  ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT  
 801  TCAATAATAT TGAAAAAGGA ACAGTATGAG TATTCAACAT TTCCGTGTCG  
 851  CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA  
 901  GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT  
 951  GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC  
1001  GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT  
1051  GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG  
1101  CATACACTAT TCTCAGAATG ACTTGGTTGA CGCGTCACCA GTCACAGAAA  
1151  AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA  
1201  ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG  
1251  ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC  
1301  GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG  
1351  CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT  
1401  AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA  
1451  TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT  
1501  GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG  
1551  TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA  
1601  TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC  
1651  GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT  
1701  TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA  
1751  GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA  
1801  CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG  
1851  ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA  
1901  AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA  
1951  ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC  
2001  TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG  
2051  CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC  
2101  AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC  
2151  GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA  
2201  GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA  
2251  TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT  
2301  AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA  
2351  ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG  
2401  CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC  
2451  CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC  
2501  ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC  
2551  GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG  
2601  CGAGTCAGTG AGCGAGGAAG CGGAAGACCG CCTGATGCGG TATTTTCTCC  
2651  TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA  
2701  CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC  
2751  TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC  
2801  GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT  
2851  GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA  
2901  AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT  
2951  TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG  
3001  AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT  
3051  TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG  
3101  GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC  
3151  TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG  
3201  CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG  
3251  CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC  
3301  TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT  
3351  CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG  
3401  GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG  
3451  TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC  
3501  TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG  
3551  CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT  
3601  GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT  
3651  TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT  
3701  CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG  
3751  AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA  
3801  TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA  
3851  TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA  
3901  TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC  
3951  GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC  
4001  GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG  
4051  GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC  
4101  GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC  
4151  CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG  
4201  AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT  
4251  AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG  
4301  GGTTGAAGGC TCTCAAGGGC ATCGGTCGAG ATCCCGGTGC CTAATGAGTG  
4351  AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG  
4401  AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG  
4451  GCGGTTTGCG TATTGGGCGC CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC  
4501  GGGCAACAGC TGATTGCCCT TCACCGCCTG GCCCTGAGAG AGTTGCAGCA  
4551  AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG TTTGATGGTG  
4601  GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC  
4651  TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA  
4701  TTGCGCCCAG CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG  
4751  ATGCCCTCAT TCAGCATTTG CATGGTTTGT TGAAAACCGG ACATGGCACT  
4801  CCAGTCGCCT TCCCGTTCCG CTATCGGCTG AATTTGATTG CGAGTGAGAT  
4851  ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA ACTTAATGGG  
4901  CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC  
4951  GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG  
5001  TCTGGTCAGA GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT  
5051  TCCACAGCAA TGGCATCCTG GTCATCCAGC GGATAGTTAA TGATCAGCCC  
5101  ACTGACGCGT TGCGCGAGAA GATTGTGCAC CGCCGCTTTA CAGGCTTCGA  
5151  CGCCGCTTCG TTCTACCATC GACACCACCA CGCTGGCACC CAGTTGATCG  
5201  GCGCGAGATT TAATCGCCGC GACAATTTGC GACGGCGCGT GCAGGGCCAG  
5251  ACTGGAGGTG GCAACGCCAA TCAGCAACGA CTGTTTGCCC GCCAGTTGTT  
5301  GTGCCACGCG GTTGGGAATG TAATTCAGCT CCGCCATCGC CGCTTCCACT  
5351  TTTTCCCGCG TTTTCGCAGA AACGTGGCTG GCCTGGTTCA CCACGCGGGA  
5401  AACGGTCTGA TAAGAGACAC CGGCATACTC TGCGACATCG TATAACGTTA  
5451  CTGGTTTCAC ATTCACCACC CTGAATTGAC TGTCTTCCGG GCGCTATCAT  
5501  GCCATACCGC GAAAGGTTTT GCGCCATTCG ATGGTGTCCG GGATCTCGAC  
5551  GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG  
5601  AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC  
5651  GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC  
5701  AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG  
5751  TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC  
5801  CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT

@@ Line 1: / Line 1: @@
-==At a Glance {{HelpIcon|Help:Plasmid}}==
+==At a Glance==
-[[Image:Pbenset2a.JPG|thumb|right|300px|pBEn-SET2a From [http://www.stratagene.com/vectors/maps/pdf/pBEn-SET2a.pdf STRATAGENE]]]
+[[Image:Pbenset2a.JPG|thumb|right|300px|pBEn-SET2a Image provided by [http://www.genomics.agilent.com/files/Vectors/Maps/pdf/pBEn-SET2a.pdf Agilent-Genomics]]]
 <protect><!--box uid=63ce1649aab8b3180235b0af7618f667.0.Z46f3a860bbf0d-->
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-{|{{Prettytable}} width='30%' align=left
+{| {{Prettytable}} width='30%' align=left  id="Z46f3a860bbf0d"  class=" tableEdit PlasmidTable"
 |-
-!{{table heading style}} |Type
+!align=left {{table heading style}} |Type
-|
+||
 Expression
 |-
-!{{table heading style}} |Origin of Replication
+!align=left {{table heading style}} |Origin of Replication
-|
+||
 pBR322
+|-
+!align=left {{table heading style}} |Copy #
+||
-–2501
 |-
-!{{table heading style}} |Copy #
+!align=left {{table heading style}} |Markers
-|
+||
+Ampicillin
 |-
-!{{table heading style}} |Markers
+!align=left {{table heading style}} |Link to Sequence
-|
+||
+[http://www.genomics.agilent.com/files/Vectors/Sequences/pBEn-SET2a_s.txt Agilent-Genomics]
 |-
-!{{table heading style}} |Link to Sequence
+!align=left {{table heading style}} |Features
-|
+||
-[http://www.stratagene.com/vectors/sequences/pBEn-SET2c_s.txt Sequence at STRATAGENE]
+T7 Promoter<br />
+lac Operator<br />
+extended RBS<br />
+SET2 tag<br />
+MCS<br />
+lacI repressor ORF
 |-
-!{{table heading style}} |Comments
+!align=left {{table heading style}} |Accession Number
-|
+||
-|-
-|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.Z46f3a860bbf0d&page=0&pagename={{FULLPAGENAMEE}} edit table]
+|- class="tableEdit_footer"
+|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.Z46f3a860bbf0d&page=112555&pagename={{FULLPAGENAMEE}}&type=1&template=PlasmidTable edit table]</span> ||
 |}
 <!--box uid=63ce1649aab8b3180235b0af7618f667.0.Z46f3a860bbf0d--></protect>
 <BR>
 <Br clear='all'>
+==Notes==
+Use pBEn vectors when want to use VariFlex tags at N-terminus of protein of interest.
 ==History {{HelpIcon|Help:Plasmid}}==
 {{PlasmidHistory}}
-==Sources {{HelpIcon|Help:Plasmid}}==
+==Sources==
-{{PlasmidSources}}
+[http://www.stratagene.com/lit/vector.aspx Agilent-Genomics]
 ==References==
+{{BacktoTop}}
 {{RefHelp}}
-<references/>
+<references />
 ==Sequence==
@@ Line 59: / Line 72: @@
   GAGGCGAGTG TTACTTCAAC AGAAGAAACC TTAACGCCAG CACAGGAGGC
   CGCAGAGACC GAAGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGAGG
-  CTGAAACCGC TGAGCAACTG CTGGTTCCGC GTGGATCTGC GGATCCGAAT
+  CTGAAACCGC TGAGCAACTG CTGGTTCCGC GTGGATCTGG ATCCGAATTC
-  TCTCTTCCCG GGTCTTGTTC CATGGGTCGA CTCGAGCTCA AGCTTAGATC
+  TCTTCCCGGG TCTTGTTCCA TGGGTCGACT CGAGCTCAAG CTTAGATCCG
-  CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT
+  GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA
-  GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG
+  GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT
-  TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCCGCA AGAGGCCCGG
+  TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCCGCAAG AGGCCCGGCA
-  CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC
+  GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG
-  CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG
+  AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG
-  CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT AATCGAAGAT
+  TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTAA TCGAAGATAA
-  AAGCTGTCAA ACATGACAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC
+  GCTGTCAAAC ATGACAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT
-  CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG
+  ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG
-  TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT
+  GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA
-  AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG
+  ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT
-  CTTCAATAAT ATTGAAAAAG GAACAGTATG AGTATTCAAC ATTTCCGTGT
+  TCAATAATAT TGAAAAAGGA ACAGTATGAG TATTCAACAT TTCCGTGTCG
-  CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC
+  CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA
-  CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA
+  GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT
-  GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT
+  GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC
-  TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT
+  GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT
-  GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC
+  GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG
-  CGCATACACT ATTCTCAGAA TGACTTGGTT GACGCGTCAC CAGTCACAGA
+  CATACACTAT TCTCAGAATG ACTTGGTTGA CGCGTCACCA GTCACAGAAA
-  AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA
+  AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA
-  TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA
+  ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG
-  GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC
+  ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC
-  TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG
+  GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG
-  AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA
+  CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT
-  TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG
+  AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA
-  GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG
+  TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT
-  CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC
+  GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG
-  GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT
+  TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA
-  TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA
+  TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC
-  TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA
+  GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT
-  GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA
+  TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA
-  AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT
+  GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA
-  AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA
+  CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG
-  GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC
+  ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA
-  AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC
+  AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA
-  CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT
+  ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC
-  ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT
+  TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG
-  AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG
+  CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC
-  CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA
+  AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC
-  CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC
+  GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA
-  CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC
+  GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA
-  TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG
+  TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT
-  GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG
+  AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA
-  AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG
+  ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG
-  AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC
+  CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC
-  GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC
+  CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC
-  TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA
+  ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC
-  CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC
+  GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG
-  AGCGAGTCAG TGAGCGAGGA AGCGGAAGAC CGCCTGATGC GGTATTTTCT
+  CGAGTCAGTG AGCGAGGAAG CGGAAGACCG CCTGATGCGG TATTTTCTCC
-  CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG
+  TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA
-  TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC
+  CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC
-  GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG
+  TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC
-  ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT
+  GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT
-  GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC
+  GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA
-  GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG
+  AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT
-  ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC
+  TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG
-  AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT
+  AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT
-  TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA
+  TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG
-  TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT
+  GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC
-  ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT
+  TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG
-  GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC
+  CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG
-  AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT
+  CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC
-  CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT
+  TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT
-  TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC
+  CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG
-  AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC
+  GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG
-  GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT
+  TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC
-  CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC
+  TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG
-  TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT
+  CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT
-  ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG
+  GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT
-  ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC
+  TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT
-  TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG
+  CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG
-  GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC
+  AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA
-  CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT
+  TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA
-  GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT
+  TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA
-  GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC
+  TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC
-  CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC
+  GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC
-  TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC
+  GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG
-  CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG
+  GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC
-  ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG
+  GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC
-  GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC
+  CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG
-  AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC
+  AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT
-  ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC
+  AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG
-  TGGGTTGAAG GCTCTCAAGG GCATCGGTCG AGATCCCGGT GCCTAATGAG
+  GGTTGAAGGC TCTCAAGGGC ATCGGTCGAG ATCCCGGTGC CTAATGAGTG
-  TGAGCTAACT TACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG
+  AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG
-  GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG
+  AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG
-  AGGCGGTTTG CGTATTGGGC GCCAGGGTGG TTTTTCTTTT CACCAGTGAG
+  GCGGTTTGCG TATTGGGCGC CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC
-  ACGGGCAACA GCTGATTGCC CTTCACCGCC TGGCCCTGAG AGAGTTGCAG
+  GGGCAACAGC TGATTGCCCT TCACCGCCTG GCCCTGAGAG AGTTGCAGCA
-  CAAGCGGTCC ACGCTGGTTT GCCCCAGCAG GCGAAAATCC TGTTTGATGG
+  AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG TTTGATGGTG
-  TGGTTAACGG CGGGATATAA CATGAGCTGT CTTCGGTATC GTCGTATCCC
+  GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC
-  ACTACCGAGA TATCCGCACC AACGCGCAGC CCGGACTCGG TAATGGCGCG
+  TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA
-  CATTGCGCCC AGCGCCATCT GATCGTTGGC AACCAGCATC GCAGTGGGAA
+  TTGCGCCCAG CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG
-  CGATGCCCTC ATTCAGCATT TGCATGGTTT GTTGAAAACC GGACATGGCA
+  ATGCCCTCAT TCAGCATTTG CATGGTTTGT TGAAAACCGG ACATGGCACT
-  CTCCAGTCGC CTTCCCGTTC CGCTATCGGC TGAATTTGAT TGCGAGTGAG
+  CCAGTCGCCT TCCCGTTCCG CTATCGGCTG AATTTGATTG CGAGTGAGAT
-  ATATTTATGC CAGCCAGCCA GACGCAGACG CGCCGAGACA GAACTTAATG
+  ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA ACTTAATGGG
-  GGCCCGCTAA CAGCGCGATT TGCTGGTGAC CCAATGCGAC CAGATGCTCC
+  CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC
-  ACGCCCAGTC GCGTACCGTC TTCATGGGAG AAAATAATAC TGTTGATGGG
+  GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG
-  TGTCTGGTCA GAGACATCAA GAAATAACGC CGGAACATTA GTGCAGGCAG
+  TCTGGTCAGA GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT
-  CTTCCACAGC AATGGCATCC TGGTCATCCA GCGGATAGTT AATGATCAGC
+  TCCACAGCAA TGGCATCCTG GTCATCCAGC GGATAGTTAA TGATCAGCCC
-  CCACTGACGC GTTGCGCGAG AAGATTGTGC ACCGCCGCTT TACAGGCTTC
+  ACTGACGCGT TGCGCGAGAA GATTGTGCAC CGCCGCTTTA CAGGCTTCGA
-  GACGCCGCTT CGTTCTACCA TCGACACCAC CACGCTGGCA CCCAGTTGAT
+  CGCCGCTTCG TTCTACCATC GACACCACCA CGCTGGCACC CAGTTGATCG
-  CGGCGCGAGA TTTAATCGCC GCGACAATTT GCGACGGCGC GTGCAGGGCC
+  GCGCGAGATT TAATCGCCGC GACAATTTGC GACGGCGCGT GCAGGGCCAG
-  AGACTGGAGG TGGCAACGCC AATCAGCAAC GACTGTTTGC CCGCCAGTTG
+  ACTGGAGGTG GCAACGCCAA TCAGCAACGA CTGTTTGCCC GCCAGTTGTT
-  TTGTGCCACG CGGTTGGGAA TGTAATTCAG CTCCGCCATC GCCGCTTCCA
+  GTGCCACGCG GTTGGGAATG TAATTCAGCT CCGCCATCGC CGCTTCCACT
-  CTTTTTCCCG CGTTTTCGCA GAAACGTGGC TGGCCTGGTT CACCACGCGG
+  TTTTCCCGCG TTTTCGCAGA AACGTGGCTG GCCTGGTTCA CCACGCGGGA
-  GAAACGGTCT GATAAGAGAC ACCGGCATAC TCTGCGACAT CGTATAACGT
+  AACGGTCTGA TAAGAGACAC CGGCATACTC TGCGACATCG TATAACGTTA
-  TACTGGTTTC ACATTCACCA CCCTGAATTG ACTGTCTTCC GGGCGCTATC
+  CTGGTTTCAC ATTCACCACC CTGAATTGAC TGTCTTCCGG GCGCTATCAT
-  ATGCCATACC GCGAAAGGTT TTGCGCCATT CGATGGTGTC CGGGATCTCG
+  GCCATACCGC GAAAGGTTTT GCGCCATTCG ATGGTGTCCG GGATCTCGAC
-  ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT
+  GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG
-  TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG
+  AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC
-  GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA
+  GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC
-  ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA
+  AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG
-  TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG
+  TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC
-  GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT
+  CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT
-[[Category:Plasmids]]
+[[Category:Plasmids]][[Category:Expression Plasmids]][[Category:Stratagene Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:T7 Promoter]][[Category:lac Operator]][[Category:MCS]][[Category:Agilent Plasmids]]
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