pMAL-c4X: Difference between revisions

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==At a Glance {{HelpIcon|Help:Plasmid}}==
==At a Glance==
[[Image:PMAL-c4x.jpg|thumb|400px|right||pMAL-c4X. In red is the '''bla''' gene, with the origins of replication in green,the MCS in cyan, and the promoter sites in magenta ]]
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{|{{Prettytable}} width='30%' align=left
{| {{Prettytable}} width='30%' align=left id="K46e6c860acb96"  class=" tableEdit PlasmidTable" 
 
|-
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!{{table heading style}} |Type
!align=left {{table heading style}} |Type
||
Maltose binding protein (MBP) vector <ref name='PMID:3073105'/>
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!{{table heading style}} |Origin of Replication
!align=left {{table heading style}} |Origin of Replication
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M13, pMB1
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!{{table heading style}} |Copy #
!align=left {{table heading style}} |Copy #
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|-
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!{{table heading style}} |Markers
!align=left {{table heading style}} |Markers
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Ampicillin
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!{{table heading style}} |Link to Sequence
!align=left {{table heading style}} |Link to Sequence
||
[http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/GenBank/pMAL-c4X.gbk.txt NEB]
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!{{table heading style}} |Comments
!align=left {{table heading style}} |Features
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lacIq promoter<Br />
Ptac promoter<Br />
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!align=left {{table heading style}} |Accession Number
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==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}


==Sources {{HelpIcon|Help:Plasmid}}==
==Notes==
{{PlasmidSources}}
The vector pMAL-c4X produces maltose-binding protein fusions
 
==History==
The MBP has been engineered for tighter binding to amylose resin.
 
==Sources==
[http://www.neb.com/nebecomm/products/productN8107.asp New England Biolabs]


==References==
==References==
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{{RefHelp}}
{{RefHelp}}
<references/>
<references />


==Sequence  
==Sequence==


     1    CCGACACCAT CGAATGGTGC AAAACCTTTC GCGGTATGGC ATGATAGCGC  
     1    CCGACACCAT CGAATGGTGC AAAACCTTTC GCGGTATGGC ATGATAGCGC  
Line 48: Line 73:
     401  TAAAGCGGCG GTGCACAATC TTCTCGCGCA ACGCGTCAGT GGGCTGATCA  
     401  TAAAGCGGCG GTGCACAATC TTCTCGCGCA ACGCGTCAGT GGGCTGATCA  
     451  TTAACTATCC GCTGGATGAC CAGGATGCCA TTGCTGTGGA AGCTGCCTGC  
     451  TTAACTATCC GCTGGATGAC CAGGATGCCA TTGCTGTGGA AGCTGCCTGC  
501  ACTAATGTTC CGGCGTTATT TCTTGATGTC TCTGACCAGA CACCCATCAA  
    501  ACTAATGTTC CGGCGTTATT TCTTGATGTC TCTGACCAGA CACCCATCAA  
551  CAGTATTATT TTCTCCCATG AAGACGGTAC GCGACTGGGC GTGGAGCATC  
    551  CAGTATTATT TTCTCCCATG AAGACGGTAC GCGACTGGGC GTGGAGCATC  
601  TGGTCGCATT GGGTCACCAG CAAATCGCGC TGTTAGCGGG CCCATTAAGT  
    601  TGGTCGCATT GGGTCACCAG CAAATCGCGC TGTTAGCGGG CCCATTAAGT  
651  TCTGTCTCGG CGCGTCTGCG TCTGGCTGGC TGGCATAAAT ATCTCACTCG  
    651  TCTGTCTCGG CGCGTCTGCG TCTGGCTGGC TGGCATAAAT ATCTCACTCG  
701  CAATCAAATT CAGCCGATAG CGGAACGGGA AGGCGACTGG AGTGCCATGT  
    701  CAATCAAATT CAGCCGATAG CGGAACGGGA AGGCGACTGG AGTGCCATGT  
751  CCGGTTTTCA ACAAACCATG CAAATGCTGA ATGAGGGCAT CGTTCCCACT  
    751  CCGGTTTTCA ACAAACCATG CAAATGCTGA ATGAGGGCAT CGTTCCCACT  
801  GCGATGCTGG TTGCCAACGA TCAGATGGCG CTGGGCGCAA TGCGCGCCAT  
    801  GCGATGCTGG TTGCCAACGA TCAGATGGCG CTGGGCGCAA TGCGCGCCAT  
851  TACCGAGTCC GGGCTGCGCG TTGGTGCGGA TATCTCGGTA GTGGGATACG  
    851  TACCGAGTCC GGGCTGCGCG TTGGTGCGGA TATCTCGGTA GTGGGATACG  
901  ACGATACCGA AGACAGCTCA TGTTATATCC CGCCGTTAAC CACCATCAAA  
    901  ACGATACCGA AGACAGCTCA TGTTATATCC CGCCGTTAAC CACCATCAAA  
951  CAGGATTTTC GCCTGCTGGG GCAAACCAGC GTGGACCGCT TGCTGCAACT  
    951  CAGGATTTTC GCCTGCTGGG GCAAACCAGC GTGGACCGCT TGCTGCAACT  
1001  CTCTCAGGGC CAGGCGGTGA AGGGCAATCA GCTGTTGCCC GTCTCACTGG  
    1001  CTCTCAGGGC CAGGCGGTGA AGGGCAATCA GCTGTTGCCC GTCTCACTGG  
1051  TGAAAAGAAA AACCACCCTG GCGCCCAATA CGCAAACCGC CTCTCCCCGC  
    1051  TGAAAAGAAA AACCACCCTG GCGCCCAATA CGCAAACCGC CTCTCCCCGC  
1101  GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA  
    1101  GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA  
1151  AAGCGGGCAG TGAGCGCAAC GCAATTAATG TAAGTTAGCT CACTCATTAG  
    1151  AAGCGGGCAG TGAGCGCAAC GCAATTAATG TAAGTTAGCT CACTCATTAG  
1201  GCACAATTCT CATGTTTGAC AGCTTATCAT CGACTGCACG GTGCACCAAT  
    1201  GCACAATTCT CATGTTTGAC AGCTTATCAT CGACTGCACG GTGCACCAAT  
1251  GCTTCTGGCG TCAGGCAGCC ATCGGAAGCT GTGGTATGGC TGTGCAGGTC  
    1251  GCTTCTGGCG TCAGGCAGCC ATCGGAAGCT GTGGTATGGC TGTGCAGGTC  
1301  GTAAATCACT GCATAATTCG TGTCGCTCAA GGCGCACTCC CGTTCTGGAT  
    1301  GTAAATCACT GCATAATTCG TGTCGCTCAA GGCGCACTCC CGTTCTGGAT  
1351  AATGTTTTTT GCGCCGACAT CATAACGGTT CTGGCAAATA TTCTGAAATG  
    1351  AATGTTTTTT GCGCCGACAT CATAACGGTT CTGGCAAATA TTCTGAAATG  
1401  AGCTGTTGAC AATTAATCAT CGGCTCGTAT AATGTGTGGA ATTGTGAGCG  
    1401  AGCTGTTGAC AATTAATCAT CGGCTCGTAT AATGTGTGGA ATTGTGAGCG  
1451  GATAACAATT TCACACAGGA AACAGCCAGT CCGTTTAGGT GTTTTCACGA  
    1451  GATAACAATT TCACACAGGA AACAGCCAGT CCGTTTAGGT GTTTTCACGA  
1501  GCACTTCACC AACAAGGACC ATAGCATATG AAAATCGAAG AAGGTAAACT  
    1501  GCACTTCACC AACAAGGACC ATAGCATATG AAAATCGAAG AAGGTAAACT  
1551  GGTAATCTGG ATTAACGGCG ATAAAGGCTA TAACGGTCTC GCTGAAGTCG  
    1551  GGTAATCTGG ATTAACGGCG ATAAAGGCTA TAACGGTCTC GCTGAAGTCG  
1601  GTAAGAAATT CGAGAAAGAT ACCGGAATTA AAGTCACCGT TGAGCATCCG  
    1601  GTAAGAAATT CGAGAAAGAT ACCGGAATTA AAGTCACCGT TGAGCATCCG  
1651  GATAAACTGG AAGAGAAATT CCCACAGGTT GCGGCAACTG GCGATGGCCC  
    1651  GATAAACTGG AAGAGAAATT CCCACAGGTT GCGGCAACTG GCGATGGCCC  
1701  TGACATTATC TTCTGGGCAC ACGACCGCTT TGGTGGCTAC GCTCAATCTG  
    1701  TGACATTATC TTCTGGGCAC ACGACCGCTT TGGTGGCTAC GCTCAATCTG  
1751  GCCTGTTGGC TGAAATCACC CCGGACAAAG CGTTCCAGGA CAAGCTGTAT  
    1751  GCCTGTTGGC TGAAATCACC CCGGACAAAG CGTTCCAGGA CAAGCTGTAT  
1801  CCGTTTACCT GGGATGCCGT ACGTTACAAC GGCAAGCTGA TTGCTTACCC  
    1801  CCGTTTACCT GGGATGCCGT ACGTTACAAC GGCAAGCTGA TTGCTTACCC  
1851  GATCGCTGTT GAAGCGTTAT CGCTGATTTA TAACAAAGAT CTGCTGCCGA  
    1851  GATCGCTGTT GAAGCGTTAT CGCTGATTTA TAACAAAGAT CTGCTGCCGA  
1901  ACCCGCCAAA AACCTGGGAA GAGATCCCGG CGCTGGATAA AGAACTGAAA  
    1901  ACCCGCCAAA AACCTGGGAA GAGATCCCGG CGCTGGATAA AGAACTGAAA  
1951  GCGAAAGGTA AGAGCGCGCT GATGTTCAAC CTGCAAGAAC CGTACTTCAC  
    1951  GCGAAAGGTA AGAGCGCGCT GATGTTCAAC CTGCAAGAAC CGTACTTCAC  
2001  CTGGCCGCTG ATTGCTGCTG ACGGGGGTTA TGCGTTCAAG TATGAAAACG  
    2001  CTGGCCGCTG ATTGCTGCTG ACGGGGGTTA TGCGTTCAAG TATGAAAACG  
2051  GCAAGTACGA CATTAAAGAC GTGGGCGTGG ATAACGCTGG CGCGAAAGCG  
    2051  GCAAGTACGA CATTAAAGAC GTGGGCGTGG ATAACGCTGG CGCGAAAGCG  
2101  GGTCTGACCT TCCTGGTTGA CCTGATTAAA AACAAACACA TGAATGCAGA  
    2101  GGTCTGACCT TCCTGGTTGA CCTGATTAAA AACAAACACA TGAATGCAGA  
2151  CACCGATTAC TCCATCGCAG AAGCTGCCTT TAATAAAGGC GAAACAGCGA  
    2151  CACCGATTAC TCCATCGCAG AAGCTGCCTT TAATAAAGGC GAAACAGCGA  
2201  TGACCATCAA CGGCCCGTGG GCATGGTCCA ACATCGACAC CAGCAAAGTG  
    2201  TGACCATCAA CGGCCCGTGG GCATGGTCCA ACATCGACAC CAGCAAAGTG  
2251  AATTATGGTG TAACGGTACT GCCGACCTTC AAGGGTCAAC CATCCAAACC  
    2251  AATTATGGTG TAACGGTACT GCCGACCTTC AAGGGTCAAC CATCCAAACC  
2301  GTTCGTTGGC GTGCTGAGCG CAGGTATTAA CGCCGCCAGT CCGAACAAAG  
    2301  GTTCGTTGGC GTGCTGAGCG CAGGTATTAA CGCCGCCAGT CCGAACAAAG  
2351  AGCTGGCAAA AGAGTTCCTC GAAAACTATC TGCTGACTGA TGAAGGTCTG  
    2351  AGCTGGCAAA AGAGTTCCTC GAAAACTATC TGCTGACTGA TGAAGGTCTG  
2401  GAAGCGGTTA ATAAAGACAA ACCGCTGGGT GCCGTAGCGC TGAAGTCTTA  
    2401  GAAGCGGTTA ATAAAGACAA ACCGCTGGGT GCCGTAGCGC TGAAGTCTTA  
2451  CGAGGAAGAG TTGGTGAAAG ATCCGCGGAT TGCCGCCACT ATGGAAAACG  
    2451  CGAGGAAGAG TTGGTGAAAG ATCCGCGGAT TGCCGCCACT ATGGAAAACG  
2501  CCCAGAAAGG TGAAATCATG CCGAACATCC CGCAGATGTC CGCTTTCTGG  
    2501  CCCAGAAAGG TGAAATCATG CCGAACATCC CGCAGATGTC CGCTTTCTGG  
2551  TATGCCGTGC GTACTGCGGT GATCAACGCC GCCAGCGGTC GTCAGACTGT  
    2551  TATGCCGTGC GTACTGCGGT GATCAACGCC GCCAGCGGTC GTCAGACTGT  
2601  CGATGAAGCC CTGAAAGACG CGCAGACTAA TTCGAGCTCG AACAACAACA  
    2601  CGATGAAGCC CTGAAAGACG CGCAGACTAA TTCGAGCTCG AACAACAACA  
2651  ACAATAACAA TAACAACAAC CTCGGGATCG AGGGAAGGAT TTCAGAATTC  
    2651  ACAATAACAA TAACAACAAC CTCGGGATCG AGGGAAGGAT TTCAGAATTC  
2701  GGATCCTCTA GAGTCGACCT GCAGGCAAGC TTGGCACTGG CCGTCGTTTT  
    2701  GGATCCTCTA GAGTCGACCT GCAGGCAAGC TTGGCACTGG CCGTCGTTTT  
2751  ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG  
    2751  ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG  
2801  CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC  
    2801  CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC  
2851  GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGCAGCTTGG  
    2851  GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGCAGCTTGG  
2901  CTGTTTTGGC GGATGAGATA AGATTTTCAG CCTGATACAG ATTAAATCAG  
    2901  CTGTTTTGGC GGATGAGATA AGATTTTCAG CCTGATACAG ATTAAATCAG  
2951  AACGCAGAAG CGGTCTGATA AAACAGAATT TGCCTGGCGG CAGTAGCGCG  
    2951  AACGCAGAAG CGGTCTGATA AAACAGAATT TGCCTGGCGG CAGTAGCGCG  
3001  GTGGTCCCAC CTGACCCCAT GCCGAACTCA GAAGTGAAAC GCCGTAGCGC  
    3001  GTGGTCCCAC CTGACCCCAT GCCGAACTCA GAAGTGAAAC GCCGTAGCGC  
3051  CGATGGTAGT GTGGGGTCTC CCCATGCGAG AGTAGGGAAC TGCCAGGCAT  
    3051  CGATGGTAGT GTGGGGTCTC CCCATGCGAG AGTAGGGAAC TGCCAGGCAT  
3101  CAAATAAAAC GAAAGGCTCA GTCGAAAGAC TGGGCCTTTC GTTTTATCTG  
    3101  CAAATAAAAC GAAAGGCTCA GTCGAAAGAC TGGGCCTTTC GTTTTATCTG  
3151  TTGTTTGTCG GTGAACGCTC TCCTGAGTAG GACAAATCCG CCGGGAGCGG  
    3151  TTGTTTGTCG GTGAACGCTC TCCTGAGTAG GACAAATCCG CCGGGAGCGG  
3201  ATTTGAACGT TGCGAAGCAA CGGCCCGGAG GGTGGCGGGC AGGACGCCCG  
    3201  ATTTGAACGT TGCGAAGCAA CGGCCCGGAG GGTGGCGGGC AGGACGCCCG  
3251  CCATAAACTG CCAGGCATCA AATTAAGCAG AAGGCCATCC TGACGGATGG  
    3251  CCATAAACTG CCAGGCATCA AATTAAGCAG AAGGCCATCC TGACGGATGG  
3301  CCTTTTTGCG TTTCTACAAA CTCTTTTGTT TATTTTTCTA AATACATTCA  
    3301  CCTTTTTGCG TTTCTACAAA CTCTTTTGTT TATTTTTCTA AATACATTCA  
3351  AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA  
    3351  AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA  
3401  TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC  
    3401  TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC  
3451  CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG  
    3451  CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG  
3501  GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT  
    3501  GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT  
3551  CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG  
    3551  CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG  
3601  AACGTTTCCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA  
    3601  AACGTTTCCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA  
3651  TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA  
    3651  TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA  
3701  TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA  
    3701  TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA  
3751  CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT  
    3751  CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT  
3801  GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA  
    3801  GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA  
3851  GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC  
    3851  GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC  
3901  GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC  
    3901  GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC  
3951  ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA  
    3951  ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA  
4001  ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG  
    4001  ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG  
4051  ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT  
    4051  ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT  
4101  ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC  
    4101  ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC  
4151  AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA  
    4151  AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA  
4201  CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA  
    4201  CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA  
4251  GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA  
    4251  GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA  
4301  TATACTTTAG ATTGATTTAC CCCGGTTGAT AATCAGAAAA GCCCCAAAAA  
    4301  TATACTTTAG ATTGATTTAC CCCGGTTGAT AATCAGAAAA GCCCCAAAAA  
4351  CAGGAAGATT GTATAAGCAA ATATTTAAAT TGTAAACGTT AATATTTTGT  
    4351  CAGGAAGATT GTATAAGCAA ATATTTAAAT TGTAAACGTT AATATTTTGT  
4401  TAAAATTCGC GTTAAATTTT TGTTAAATCA GCTCATTTTT TAACCAATAG  
    4401  TAAAATTCGC GTTAAATTTT TGTTAAATCA GCTCATTTTT TAACCAATAG  
4451  GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGA CCGAGATAGG  
    4451  GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGA CCGAGATAGG  
4501  GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA AAGAACGTGG  
    4501  GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA AAGAACGTGG  
4551  ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA TGGCCCACTA  
    4551  ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA TGGCCCACTA  
4601  CGTGAACCAT CACCCAAATC AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC  
    4601  CGTGAACCAT CACCCAAATC AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC  
4651  ACTAAATCGG AACCCTAAAG GGAGCCCCCG ATTTAGAGCT TGACGGGGAA  
    4651  ACTAAATCGG AACCCTAAAG GGAGCCCCCG ATTTAGAGCT TGACGGGGAA  
4701  AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGC  
    4701  AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGC  
4751  GCTAGGGCGC TGGCAAGTGT AGCGGTCACG CTGCGCGTAA CCACCACACC  
    4751  GCTAGGGCGC TGGCAAGTGT AGCGGTCACG CTGCGCGTAA CCACCACACC  
4801  CGCCGCGCTT AATGCGCCGC TACAGGGCGC GTAAAAGGAT CTAGGTGAAG  
    4801  CGCCGCGCTT AATGCGCCGC TACAGGGCGC GTAAAAGGAT CTAGGTGAAG  
4851  ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT  
    4851  ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT  
4901  CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC  
    4901  CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC  
4951  CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA  
    4951  CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA  
5001  CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA  
    5001  CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA  
5051  GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT  
    5051  GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT  
5101  AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC  
    5101  AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC  
5151  CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC  
    5151  CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC  
5201  GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC  
    5201  GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC  
5251  GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG  
    5251  GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG  
5301  ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC  
    5301  ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC  
5351  GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG  
    5351  GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG  
5401  GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT  
    5401  GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT  
5451  TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG  
    5451  TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG  
5501  ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT  
    5501  ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT  
5551  TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT  
    5551  TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT  
5601  GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC  
    5601  GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC  
5651  TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG  
    5651  TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG  
5701  AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC  
    5701  AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC  
5751  GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT  
    5751  GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT  
5801  GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC  
    5801  GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC  
5851  ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC  
    5851  ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC  
5901  TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA  
    5901  TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA  
5951  GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG  
    5951  GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG  
6001  CTGCGGTAAA GCTCATCAGC GTGGTCGTGC AGCGATTCAC AGATGTCTGC  
    6001  CTGCGGTAAA GCTCATCAGC GTGGTCGTGC AGCGATTCAC AGATGTCTGC  
6051  CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT  
    6051  CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT  
6101  GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC  
    6101  GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC  
6151  ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC  
    6151  ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC  
6201  GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG  
    6201  GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG  
6251  CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG  
    6251  CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG  
6301  CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC  
    6301  CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC  
6351  AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC  
    6351  AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC  
6401  GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA  
    6401  GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA  
6451  ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT  
    6451  ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT  
6501  GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT  
    6501  GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT  
6551  AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC  
    6551  AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC  
6601  ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AAATT
    6601  ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AAATT
__NOTOC__
__NOTOC__
[[Category:Plasmids]][[Category:NEB Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:lacIq promoter]][[Category:Ptac promoter]]

Latest revision as of 16:35, 19 August 2011

At a Glance

pMAL-c4X. In red is the bla gene, with the origins of replication in green,the MCS in cyan, and the promoter sites in magenta
Type

Maltose binding protein (MBP) vector [1]

Origin of Replication

M13, pMB1

Copy #
Markers

Ampicillin

Link to Sequence

NEB

Features

lacIq promoter
Ptac promoter

Accession Number


edit table



Notes

The vector pMAL-c4X produces maltose-binding protein fusions

History

The MBP has been engineered for tighter binding to amylose resin.

Sources

New England Biolabs

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Maina, CV et al. (1988) An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding protein. Gene 74 365-73 PubMed

Sequence

   1     CCGACACCAT CGAATGGTGC AAAACCTTTC GCGGTATGGC ATGATAGCGC 
   51    CCGGAAGAGA GTCAATTCAG GGTGGTGAAT GTGAAACCAG TAACGTTATA 
   101   CGATGTCGCA GAGTATGCCG GTGTCTCTTA TCAGACCGTT TCCCGCGTGG 
   151   TGAACCAGGC CAGCCACGTT TCTGCGAAAA CGCGGGAAAA AGTGGAAGCG 
   201   GCGATGGCGG AGCTGAATTA CATTCCCAAC CGCGTGGCAC AACAACTGGC 
   251   GGGCAAACAG TCGTTGCTGA TTGGCGTTGC CACCTCCAGT CTGGCCCTGC 
   301   ACGCGCCGTC GCAAATTGTC GCGGCGATTA AATCTCGCGC CGATCAACTG 
   351   GGTGCCAGCG TGGTGGTGTC GATGGTAGAA CGAAGCGGCG TCGAAGCCTG 
   401   TAAAGCGGCG GTGCACAATC TTCTCGCGCA ACGCGTCAGT GGGCTGATCA 
   451   TTAACTATCC GCTGGATGAC CAGGATGCCA TTGCTGTGGA AGCTGCCTGC 
   501   ACTAATGTTC CGGCGTTATT TCTTGATGTC TCTGACCAGA CACCCATCAA 
   551   CAGTATTATT TTCTCCCATG AAGACGGTAC GCGACTGGGC GTGGAGCATC 
   601   TGGTCGCATT GGGTCACCAG CAAATCGCGC TGTTAGCGGG CCCATTAAGT 
   651   TCTGTCTCGG CGCGTCTGCG TCTGGCTGGC TGGCATAAAT ATCTCACTCG 
   701   CAATCAAATT CAGCCGATAG CGGAACGGGA AGGCGACTGG AGTGCCATGT 
   751   CCGGTTTTCA ACAAACCATG CAAATGCTGA ATGAGGGCAT CGTTCCCACT 
   801   GCGATGCTGG TTGCCAACGA TCAGATGGCG CTGGGCGCAA TGCGCGCCAT 
   851   TACCGAGTCC GGGCTGCGCG TTGGTGCGGA TATCTCGGTA GTGGGATACG 
   901   ACGATACCGA AGACAGCTCA TGTTATATCC CGCCGTTAAC CACCATCAAA 
   951   CAGGATTTTC GCCTGCTGGG GCAAACCAGC GTGGACCGCT TGCTGCAACT 
   1001  CTCTCAGGGC CAGGCGGTGA AGGGCAATCA GCTGTTGCCC GTCTCACTGG 
   1051  TGAAAAGAAA AACCACCCTG GCGCCCAATA CGCAAACCGC CTCTCCCCGC 
   1101  GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA 
   1151  AAGCGGGCAG TGAGCGCAAC GCAATTAATG TAAGTTAGCT CACTCATTAG 
   1201  GCACAATTCT CATGTTTGAC AGCTTATCAT CGACTGCACG GTGCACCAAT 
   1251  GCTTCTGGCG TCAGGCAGCC ATCGGAAGCT GTGGTATGGC TGTGCAGGTC 
   1301  GTAAATCACT GCATAATTCG TGTCGCTCAA GGCGCACTCC CGTTCTGGAT 
   1351  AATGTTTTTT GCGCCGACAT CATAACGGTT CTGGCAAATA TTCTGAAATG 
   1401  AGCTGTTGAC AATTAATCAT CGGCTCGTAT AATGTGTGGA ATTGTGAGCG 
   1451  GATAACAATT TCACACAGGA AACAGCCAGT CCGTTTAGGT GTTTTCACGA 
   1501  GCACTTCACC AACAAGGACC ATAGCATATG AAAATCGAAG AAGGTAAACT 
   1551  GGTAATCTGG ATTAACGGCG ATAAAGGCTA TAACGGTCTC GCTGAAGTCG 
   1601  GTAAGAAATT CGAGAAAGAT ACCGGAATTA AAGTCACCGT TGAGCATCCG 
   1651  GATAAACTGG AAGAGAAATT CCCACAGGTT GCGGCAACTG GCGATGGCCC 
   1701  TGACATTATC TTCTGGGCAC ACGACCGCTT TGGTGGCTAC GCTCAATCTG 
   1751  GCCTGTTGGC TGAAATCACC CCGGACAAAG CGTTCCAGGA CAAGCTGTAT 
   1801  CCGTTTACCT GGGATGCCGT ACGTTACAAC GGCAAGCTGA TTGCTTACCC 
   1851  GATCGCTGTT GAAGCGTTAT CGCTGATTTA TAACAAAGAT CTGCTGCCGA 
   1901  ACCCGCCAAA AACCTGGGAA GAGATCCCGG CGCTGGATAA AGAACTGAAA 
   1951  GCGAAAGGTA AGAGCGCGCT GATGTTCAAC CTGCAAGAAC CGTACTTCAC 
   2001  CTGGCCGCTG ATTGCTGCTG ACGGGGGTTA TGCGTTCAAG TATGAAAACG 
   2051  GCAAGTACGA CATTAAAGAC GTGGGCGTGG ATAACGCTGG CGCGAAAGCG 
   2101  GGTCTGACCT TCCTGGTTGA CCTGATTAAA AACAAACACA TGAATGCAGA 
   2151  CACCGATTAC TCCATCGCAG AAGCTGCCTT TAATAAAGGC GAAACAGCGA 
   2201  TGACCATCAA CGGCCCGTGG GCATGGTCCA ACATCGACAC CAGCAAAGTG 
   2251  AATTATGGTG TAACGGTACT GCCGACCTTC AAGGGTCAAC CATCCAAACC 
   2301  GTTCGTTGGC GTGCTGAGCG CAGGTATTAA CGCCGCCAGT CCGAACAAAG 
   2351  AGCTGGCAAA AGAGTTCCTC GAAAACTATC TGCTGACTGA TGAAGGTCTG 
   2401  GAAGCGGTTA ATAAAGACAA ACCGCTGGGT GCCGTAGCGC TGAAGTCTTA 
   2451  CGAGGAAGAG TTGGTGAAAG ATCCGCGGAT TGCCGCCACT ATGGAAAACG 
   2501  CCCAGAAAGG TGAAATCATG CCGAACATCC CGCAGATGTC CGCTTTCTGG 
   2551  TATGCCGTGC GTACTGCGGT GATCAACGCC GCCAGCGGTC GTCAGACTGT 
   2601  CGATGAAGCC CTGAAAGACG CGCAGACTAA TTCGAGCTCG AACAACAACA 
   2651  ACAATAACAA TAACAACAAC CTCGGGATCG AGGGAAGGAT TTCAGAATTC 
   2701  GGATCCTCTA GAGTCGACCT GCAGGCAAGC TTGGCACTGG CCGTCGTTTT 
   2751  ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG 
   2801  CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC 
   2851  GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGCAGCTTGG 
   2901  CTGTTTTGGC GGATGAGATA AGATTTTCAG CCTGATACAG ATTAAATCAG 
   2951  AACGCAGAAG CGGTCTGATA AAACAGAATT TGCCTGGCGG CAGTAGCGCG 
   3001  GTGGTCCCAC CTGACCCCAT GCCGAACTCA GAAGTGAAAC GCCGTAGCGC 
   3051  CGATGGTAGT GTGGGGTCTC CCCATGCGAG AGTAGGGAAC TGCCAGGCAT 
   3101  CAAATAAAAC GAAAGGCTCA GTCGAAAGAC TGGGCCTTTC GTTTTATCTG 
   3151  TTGTTTGTCG GTGAACGCTC TCCTGAGTAG GACAAATCCG CCGGGAGCGG 
   3201  ATTTGAACGT TGCGAAGCAA CGGCCCGGAG GGTGGCGGGC AGGACGCCCG 
   3251  CCATAAACTG CCAGGCATCA AATTAAGCAG AAGGCCATCC TGACGGATGG 
   3301  CCTTTTTGCG TTTCTACAAA CTCTTTTGTT TATTTTTCTA AATACATTCA 
   3351  AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA 
   3401  TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC 
   3451  CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG 
   3501  GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT 
   3551  CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG 
   3601  AACGTTTCCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA 
   3651  TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA 
   3701  TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA 
   3751  CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT 
   3801  GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA 
   3851  GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC 
   3901  GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC 
   3951  ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA 
   4001  ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG 
   4051  ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT 
   4101  ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC 
   4151  AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA 
   4201  CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA 
   4251  GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA 
   4301  TATACTTTAG ATTGATTTAC CCCGGTTGAT AATCAGAAAA GCCCCAAAAA 
   4351  CAGGAAGATT GTATAAGCAA ATATTTAAAT TGTAAACGTT AATATTTTGT 
   4401  TAAAATTCGC GTTAAATTTT TGTTAAATCA GCTCATTTTT TAACCAATAG 
   4451  GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGA CCGAGATAGG 
   4501  GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA AAGAACGTGG 
   4551  ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA TGGCCCACTA 
   4601  CGTGAACCAT CACCCAAATC AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC 
   4651  ACTAAATCGG AACCCTAAAG GGAGCCCCCG ATTTAGAGCT TGACGGGGAA 
   4701  AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGC 
   4751  GCTAGGGCGC TGGCAAGTGT AGCGGTCACG CTGCGCGTAA CCACCACACC 
   4801  CGCCGCGCTT AATGCGCCGC TACAGGGCGC GTAAAAGGAT CTAGGTGAAG 
   4851  ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT 
   4901  CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC 
   4951  CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA 
   5001  CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA 
   5051  GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT 
   5101  AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC 
   5151  CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC 
   5201  GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC 
   5251  GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG 
   5301  ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC 
   5351  GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG 
   5401  GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT 
   5451  TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG 
   5501  ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT 
   5551  TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT 
   5601  GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC 
   5651  TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG 
   5701  AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC 
   5751  GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT 
   5801  GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC 
   5851  ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC 
   5901  TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA 
   5951  GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG 
   6001  CTGCGGTAAA GCTCATCAGC GTGGTCGTGC AGCGATTCAC AGATGTCTGC 
   6051  CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT 
   6101  GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC 
   6151  ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC 
   6201  GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG 
   6251  CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG 
   6301  CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC 
   6351  AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC 
   6401  GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA 
   6451  ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT 
   6501  GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT 
   6551  AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC 
   6601  ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AAATT
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