pMAL-p4G: Difference between revisions

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==At a Glance {{HelpIcon|Help:Plasmid}}==
==At a Glance==
 
[[File:PMAL-p4G.jpg|thumb|450px|pMAL-p4G, Image made with [[PlasMapper]]]]
 
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{|{{Prettytable}} width='30%' align=left
{| {{Prettytable}} width='30%' align=left id="L46e17257c9410"  class=" tableEdit PlasmidTable" 
 
|-
|-
!{{table heading style}} |Type
!align=left {{table heading style}} |Type
|
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Cloning
Fusion
|-
|-
!{{table heading style}} |Origin of Replication
!align=left {{table heading style}} |Origin of Replication
|
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M13; pMB1
M13; pMB1
|-
|-
!{{table heading style}} |Copy #
!align=left {{table heading style}} |Copy #
|
||


|-
|-
!{{table heading style}} |Markers
!align=left {{table heading style}} |Markers
|
||
 
Ampicillin
|-
!align=left {{table heading style}} |Link to Sequence
||
[http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/pmalp4g.txt  NEB]
|-
|-
!{{table heading style}} |Link to Sequence
!align=left {{table heading style}} |Features
|
||
[http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/pmalp4g.txt Sequence at NEB]
lac Iq Promoter<br />
Ptac Promoter
|-
|-
!{{table heading style}} |Comments
!align=left {{table heading style}} |Accession Number
|
||
 


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==Notes==
pMAL-p4G is designed to produce maltose-binding protein (MBP) fusions <ref name='PMID:3073105'/>, where the protein of interest can be cleaved from MBP with the specific protease Genenase™ I.  Its source is NEB 5-alpha Competent E. coli.
==History {{HelpIcon|Help:Plasmid}}==
==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}
{{PlasmidHistory}}


==Sources {{HelpIcon|Help:Plasmid}}==
==Sources==
{{PlasmidSources}}
[http://www.neb.com/nebecomm/products/productN8103.asp New England Biolabs]


==References==
==References==
{{BacktoTop}}
{{RefHelp}}
{{RefHelp}}
<references/>
<references />
 
==Sequence {{HelpIcon|Help:Plasmid}}==
{{PlasmidSeq}}


==Sequence==


      1  CCGACACCAT CGAATGGTGC AAAACCTTTC GCGGTATGGC ATGATAGCGC
      51  CCGGAAGAGA GTCAATTCAG GGTGGTGAAT GTGAAACCAG TAACGTTATA
    101  CGATGTCGCA GAGTATGCCG GTGTCTCTTA TCAGACCGTT TCCCGCGTGG
    151  TGAACCAGGC CAGCCACGTT TCTGCGAAAA CGCGGGAAAA AGTGGAAGCG
    201  GCGATGGCGG AGCTGAATTA CATTCCCAAC CGCGTGGCAC AACAACTGGC
    251  GGGCAAACAG TCGTTGCTGA TTGGCGTTGC CACCTCCAGT CTGGCCCTGC
    301  ACGCGCCGTC GCAAATTGTC GCGGCGATTA AATCTCGCGC CGATCAACTG
    351  GGTGCCAGCG TGGTGGTGTC GATGGTAGAA CGAAGCGGCG TCGAAGCCTG
    401  TAAAGCGGCG GTGCACAATC TTCTCGCGCA ACGCGTCAGT GGGCTGATCA
    451  TTAACTATCC GCTGGATGAC CAGGATGCCA TTGCTGTGGA AGCTGCCTGC
    501  ACTAATGTTC CGGCGTTATT TCTTGATGTC TCTGACCAGA CACCCATCAA
    551  CAGTATTATT TTCTCCCATG AAGACGGTAC GCGACTGGGC GTGGAGCATC
    601  TGGTCGCATT GGGTCACCAG CAAATCGCGC TGTTAGCGGG CCCATTAAGT
    651  TCTGTCTCGG CGCGTCTGCG TCTGGCTGGC TGGCATAAAT ATCTCACTCG
    701  CAATCAAATT CAGCCGATAG CGGAACGGGA AGGCGACTGG AGTGCCATGT
    751  CCGGTTTTCA ACAAACCATG CAAATGCTGA ATGAGGGCAT CGTTCCCACT
    801  GCGATGCTGG TTGCCAACGA TCAGATGGCG CTGGGCGCAA TGCGCGCCAT
    851  TACCGAGTCC GGGCTGCGCG TTGGTGCGGA TATCTCGGTA GTGGGATACG
    901  ACGATACCGA AGACAGCTCA TGTTATATCC CGCCGTTAAC CACCATCAAA
    951  CAGGATTTTC GCCTGCTGGG GCAAACCAGC GTGGACCGCT TGCTGCAACT
    1001  CTCTCAGGGC CAGGCGGTGA AGGGCAATCA GCTGTTGCCC GTCTCACTGG
    1051  TGAAAAGAAA AACCACCCTG GCGCCCAATA CGCAAACCGC CTCTCCCCGC
    1101  GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA
    1151  AAGCGGGCAG TGAGCGCAAC GCAATTAATG TAAGTTAGCT CACTCATTAG
    1201  GCACAATTCT CATGTTTGAC AGCTTATCAT CGACTGCACG GTGCACCAAT
    1251  GCTTCTGGCG TCAGGCAGCC ATCGGAAGCT GTGGTATGGC TGTGCAGGTC
    1301  GTAAATCACT GCATAATTCG TGTCGCTCAA GGCGCACTCC CGTTCTGGAT
    1351  AATGTTTTTT GCGCCGACAT CATAACGGTT CTGGCAAATA TTCTGAAATG
    1401  AGCTGTTGAC AATTAATCAT CGGCTCGTAT AATGTGTGGA ATTGTGAGCG
    1451  GATAACAATT TCACACAGGA AACAGCCAGT CCGTTTAGGT GTTTTCACGA
    1501  GCACTTCACC AACAAGGACC ATAGCATATG AAAATAAAAA CAGGTGCACG
    1551  CATCCTCGCA TTATCCGCAT TAACGACGAT GATGTTTTCC GCCTCGGCTC
    1601  TCGCCAAAAT CGAAGAAGGT AAACTGGTAA TCTGGATTAA CGGCGATAAA
    1651  GGCTATAACG GTCTCGCTGA AGTCGGTAAG AAATTCGAGA AAGATACCGG
    1701  AATTAAAGTC ACCGTTGAGC ATCCGGATAA ACTGGAAGAG AAATTCCCAC
    1751  AGGTTGCGGC AACTGGCGAT GGCCCTGACA TTATCTTCTG GGCACACGAC
    1801  CGCTTTGGTG GCTACGCTCA ATCTGGCCTG TTGGCTGAAA TCACCCCGGA
    1851  CAAAGCGTTC CAGGACAAGC TGTATCCGTT TACCTGGGAT GCCGTACGTT
    1901  ACAACGGCAA GCTGATTGCT TACCCGATCG CTGTTGAAGC GTTATCGCTG
    1951  ATTTATAACA AAGATCTGCT GCCGAACCCG CCAAAAACCT GGGAAGAGAT
    2001  CCCGGCGCTG GATAAAGAAC TGAAAGCGAA AGGTAAGAGC GCGCTGATGT
    2051  TCAACCTGCA AGAACCGTAC TTCACCTGGC CGCTGATTGC TGCTGACGGG
    2101  GGTTATGCGT TCAAGTATGA AAACGGCAAG TACGACATTA AAGACGTGGG
    2151  CGTGGATAAC GCTGGCGCGA AAGCGGGTCT GACCTTCCTG GTTGACCTGA
    2201  TTAAAAACAA ACACATGAAT GCAGACACCG ATTACTCCAT CGCAGAAGCT
    2251  GCCTTTAATA AAGGCGAAAC AGCGATGACC ATCAACGGCC CGTGGGCATG
    2301  GTCCAACATC GACACCAGCA AAGTGAATTA TGGTGTAACG GTACTGCCGA
    2351  CCTTCAAGGG TCAACCATCC AAACCGTTCG TTGGCGTGCT GAGCGCAGGT
    2401  ATTAACGCCG CCAGTCCGAA CAAAGAGCTG GCAAAAGAGT TCCTCGAAAA
    2451  CTATCTGCTG ACTGATGAAG GTCTGGAAGC GGTTAATAAA GACAAACCGC
    2501  TGGGTGCCGT AGCGCTGAAG TCTTACGAGG AAGAGTTGGT GAAAGATCCG
    2551  CGGATTGCCG CCACTATGGA AAACGCCCAG AAAGGTGAAA TCATGCCGAA
    2601  CATCCCGCAG ATGTCCGCTT TCTGGTATGC CGTGCGTACT GCGGTGATCA
    2651  ACGCCGCCAG CGGTCGTCAG ACTGTCGATG AAGCCCTGAA AGACGCGCAG
    2701  ACTAATTCGA GCTCGAACAA CAACAACAAT AACAATAACA ACAACCTCGG
    2751  GCCGGGTGCG GCACACTACG TAGAATTCGG ATCCTCTAGA GTCGACCTGC
    2801  AGGCAAGCTT GGCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC
    2851  CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG
    2901  CTGGCGTAAT AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC
    2951  GCAGCCTGAA TGGCGAATGG CAGCTTGGCT GTTTTGGCGG ATGAGATAAG
    3001  ATTTTCAGCC TGATACAGAT TAAATCAGAA CGCAGAAGCG GTCTGATAAA
    3051  ACAGAATTTG CCTGGCGGCA GTAGCGCGGT GGTCCCACCT GACCCCATGC
    3101  CGAACTCAGA AGTGAAACGC CGTAGCGCCG ATGGTAGTGT GGGGTCTCCC
    3151  CATGCGAGAG TAGGGAACTG CCAGGCATCA AATAAAACGA AAGGCTCAGT
    3201  CGAAAGACTG GGCCTTTCGT TTTATCTGTT GTTTGTCGGT GAACGCTCTC
    3251  CTGAGTAGGA CAAATCCGCC GGGAGCGGAT TTGAACGTTG CGAAGCAACG
    3301  GCCCGGAGGG TGGCGGGCAG GACGCCCGCC ATAAACTGCC AGGCATCAAA
    3351  TTAAGCAGAA GGCCATCCTG ACGGATGGCC TTTTTGCGTT TCTACAAACT
    3401  CTTTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC
    3451  AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT
    3501  ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT
    3551  TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG
    3601  ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT
    3651  AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTCCCAA TGATGAGCAC
    3701  TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC
    3751  AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG
    3801  TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA
    3851  ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC
    3901  TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC
    3951  ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA
    4001  AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA
    4051  CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG
    4101  CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT
    4151  GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG
    4201  GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG
    4251  CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA
    4301  TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT
    4351  GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTACCC
    4401  CGGTTGATAA TCAGAAAAGC CCCAAAAACA GGAAGATTGT ATAAGCAAAT
    4451  ATTTAAATTG TAAACGTTAA TATTTTGTTA AAATTCGCGT TAAATTTTTG
    4501  TTAAATCAGC TCATTTTTTA ACCAATAGGC CGAAATCGGC AAAATCCCTT
    4551  ATAAATCAAA AGAATAGACC GAGATAGGGT TGAGTGTTGT TCCAGTTTGG
    4601  AACAAGAGTC CACTATTAAA GAACGTGGAC TCCAACGTCA AAGGGCGAAA
    4651  AACCGTCTAT CAGGGCGATG GCCCACTACG TGAACCATCA CCCAAATCAA
    4701  GTTTTTTGGG GTCGAGGTGC CGTAAAGCAC TAAATCGGAA CCCTAAAGGG
    4751  AGCCCCCGAT TTAGAGCTTG ACGGGGAAAG CCGGCGAACG TGGCGAGAAA
    4801  GGAAGGGAAG AAAGCGAAAG GAGCGGGCGC TAGGGCGCTG GCAAGTGTAG
    4851  CGGTCACGCT GCGCGTAACC ACCACACCCG CCGCGCTTAA TGCGCCGCTA
    4901  CAGGGCGCGT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA
    4951  CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA
    5001  GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG
    5051  CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG
    5101  ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG
    5151  CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT
    5201  CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC
    5251  CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA
    5301  AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC
    5351  GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC
    5401  TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG
    5451  GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA
    5501  GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC
    5551  ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC
    5601  CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG
    5651  CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG
    5701  ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA
    5751  ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT
    5801  GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG
    5851  GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA
    5901  CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC
    5951  CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT
    6001  TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC
    6051  ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT
    6101  GGTCGTGCAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG
    6151  TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT
    6201  GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG
    6251  GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC
    6301  ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA
    6351  GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC
    6401  AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT
    6451  AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC
    6501  TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC
    6551  ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT
    6601  CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA
    6651  GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC
    6701  AGGACCCAAC GCTGCCCGAA ATT




[[Category:Plasmids]]
[[Category:Plasmids]][[Category:NEB Plasmids]][[Category:Fusion Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:lacIq Promoter]][[Category:Ptac Promoter]]
__NOTOC__
__NOTOC__

Latest revision as of 16:49, 19 August 2011

At a Glance

pMAL-p4G, Image made with PlasMapper
Type

Fusion

Origin of Replication

M13; pMB1

Copy #
Markers

Ampicillin

Link to Sequence

NEB

Features

lac Iq Promoter
Ptac Promoter

Accession Number


edit table



Notes

pMAL-p4G is designed to produce maltose-binding protein (MBP) fusions [1], where the protein of interest can be cleaved from MBP with the specific protease Genenase™ I. Its source is NEB 5-alpha Competent E. coli.

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

New England Biolabs

References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. ↑ Maina, CV et al. (1988) An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding protein. Gene 74 365-73 PubMed

Sequence

      1  CCGACACCAT CGAATGGTGC AAAACCTTTC GCGGTATGGC ATGATAGCGC 
     51  CCGGAAGAGA GTCAATTCAG GGTGGTGAAT GTGAAACCAG TAACGTTATA 
    101  CGATGTCGCA GAGTATGCCG GTGTCTCTTA TCAGACCGTT TCCCGCGTGG 
    151  TGAACCAGGC CAGCCACGTT TCTGCGAAAA CGCGGGAAAA AGTGGAAGCG 
    201  GCGATGGCGG AGCTGAATTA CATTCCCAAC CGCGTGGCAC AACAACTGGC 
    251  GGGCAAACAG TCGTTGCTGA TTGGCGTTGC CACCTCCAGT CTGGCCCTGC 
    301  ACGCGCCGTC GCAAATTGTC GCGGCGATTA AATCTCGCGC CGATCAACTG 
    351  GGTGCCAGCG TGGTGGTGTC GATGGTAGAA CGAAGCGGCG TCGAAGCCTG 
    401  TAAAGCGGCG GTGCACAATC TTCTCGCGCA ACGCGTCAGT GGGCTGATCA 
    451  TTAACTATCC GCTGGATGAC CAGGATGCCA TTGCTGTGGA AGCTGCCTGC 
    501  ACTAATGTTC CGGCGTTATT TCTTGATGTC TCTGACCAGA CACCCATCAA 
    551  CAGTATTATT TTCTCCCATG AAGACGGTAC GCGACTGGGC GTGGAGCATC 
    601  TGGTCGCATT GGGTCACCAG CAAATCGCGC TGTTAGCGGG CCCATTAAGT 
    651  TCTGTCTCGG CGCGTCTGCG TCTGGCTGGC TGGCATAAAT ATCTCACTCG 
    701  CAATCAAATT CAGCCGATAG CGGAACGGGA AGGCGACTGG AGTGCCATGT 
    751  CCGGTTTTCA ACAAACCATG CAAATGCTGA ATGAGGGCAT CGTTCCCACT 
    801  GCGATGCTGG TTGCCAACGA TCAGATGGCG CTGGGCGCAA TGCGCGCCAT 
    851  TACCGAGTCC GGGCTGCGCG TTGGTGCGGA TATCTCGGTA GTGGGATACG 
    901  ACGATACCGA AGACAGCTCA TGTTATATCC CGCCGTTAAC CACCATCAAA 
    951  CAGGATTTTC GCCTGCTGGG GCAAACCAGC GTGGACCGCT TGCTGCAACT 
   1001  CTCTCAGGGC CAGGCGGTGA AGGGCAATCA GCTGTTGCCC GTCTCACTGG 
   1051  TGAAAAGAAA AACCACCCTG GCGCCCAATA CGCAAACCGC CTCTCCCCGC 
   1101  GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA 
   1151  AAGCGGGCAG TGAGCGCAAC GCAATTAATG TAAGTTAGCT CACTCATTAG 
   1201  GCACAATTCT CATGTTTGAC AGCTTATCAT CGACTGCACG GTGCACCAAT 
   1251  GCTTCTGGCG TCAGGCAGCC ATCGGAAGCT GTGGTATGGC TGTGCAGGTC 
   1301  GTAAATCACT GCATAATTCG TGTCGCTCAA GGCGCACTCC CGTTCTGGAT 
   1351  AATGTTTTTT GCGCCGACAT CATAACGGTT CTGGCAAATA TTCTGAAATG 
   1401  AGCTGTTGAC AATTAATCAT CGGCTCGTAT AATGTGTGGA ATTGTGAGCG 
   1451  GATAACAATT TCACACAGGA AACAGCCAGT CCGTTTAGGT GTTTTCACGA 
   1501  GCACTTCACC AACAAGGACC ATAGCATATG AAAATAAAAA CAGGTGCACG 
   1551  CATCCTCGCA TTATCCGCAT TAACGACGAT GATGTTTTCC GCCTCGGCTC 
   1601  TCGCCAAAAT CGAAGAAGGT AAACTGGTAA TCTGGATTAA CGGCGATAAA 
   1651  GGCTATAACG GTCTCGCTGA AGTCGGTAAG AAATTCGAGA AAGATACCGG 
   1701  AATTAAAGTC ACCGTTGAGC ATCCGGATAA ACTGGAAGAG AAATTCCCAC 
   1751  AGGTTGCGGC AACTGGCGAT GGCCCTGACA TTATCTTCTG GGCACACGAC 
   1801  CGCTTTGGTG GCTACGCTCA ATCTGGCCTG TTGGCTGAAA TCACCCCGGA 
   1851  CAAAGCGTTC CAGGACAAGC TGTATCCGTT TACCTGGGAT GCCGTACGTT 
   1901  ACAACGGCAA GCTGATTGCT TACCCGATCG CTGTTGAAGC GTTATCGCTG 
   1951  ATTTATAACA AAGATCTGCT GCCGAACCCG CCAAAAACCT GGGAAGAGAT 
   2001  CCCGGCGCTG GATAAAGAAC TGAAAGCGAA AGGTAAGAGC GCGCTGATGT 
   2051  TCAACCTGCA AGAACCGTAC TTCACCTGGC CGCTGATTGC TGCTGACGGG 
   2101  GGTTATGCGT TCAAGTATGA AAACGGCAAG TACGACATTA AAGACGTGGG 
   2151  CGTGGATAAC GCTGGCGCGA AAGCGGGTCT GACCTTCCTG GTTGACCTGA 
   2201  TTAAAAACAA ACACATGAAT GCAGACACCG ATTACTCCAT CGCAGAAGCT 
   2251  GCCTTTAATA AAGGCGAAAC AGCGATGACC ATCAACGGCC CGTGGGCATG 
   2301  GTCCAACATC GACACCAGCA AAGTGAATTA TGGTGTAACG GTACTGCCGA 
   2351  CCTTCAAGGG TCAACCATCC AAACCGTTCG TTGGCGTGCT GAGCGCAGGT 
   2401  ATTAACGCCG CCAGTCCGAA CAAAGAGCTG GCAAAAGAGT TCCTCGAAAA 
   2451  CTATCTGCTG ACTGATGAAG GTCTGGAAGC GGTTAATAAA GACAAACCGC 
   2501  TGGGTGCCGT AGCGCTGAAG TCTTACGAGG AAGAGTTGGT GAAAGATCCG 
   2551  CGGATTGCCG CCACTATGGA AAACGCCCAG AAAGGTGAAA TCATGCCGAA 
   2601  CATCCCGCAG ATGTCCGCTT TCTGGTATGC CGTGCGTACT GCGGTGATCA 
   2651  ACGCCGCCAG CGGTCGTCAG ACTGTCGATG AAGCCCTGAA AGACGCGCAG 
   2701  ACTAATTCGA GCTCGAACAA CAACAACAAT AACAATAACA ACAACCTCGG 
   2751  GCCGGGTGCG GCACACTACG TAGAATTCGG ATCCTCTAGA GTCGACCTGC 
   2801  AGGCAAGCTT GGCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC 
   2851  CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG 
   2901  CTGGCGTAAT AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC 
   2951  GCAGCCTGAA TGGCGAATGG CAGCTTGGCT GTTTTGGCGG ATGAGATAAG 
   3001  ATTTTCAGCC TGATACAGAT TAAATCAGAA CGCAGAAGCG GTCTGATAAA 
   3051  ACAGAATTTG CCTGGCGGCA GTAGCGCGGT GGTCCCACCT GACCCCATGC 
   3101  CGAACTCAGA AGTGAAACGC CGTAGCGCCG ATGGTAGTGT GGGGTCTCCC 
   3151  CATGCGAGAG TAGGGAACTG CCAGGCATCA AATAAAACGA AAGGCTCAGT 
   3201  CGAAAGACTG GGCCTTTCGT TTTATCTGTT GTTTGTCGGT GAACGCTCTC 
   3251  CTGAGTAGGA CAAATCCGCC GGGAGCGGAT TTGAACGTTG CGAAGCAACG 
   3301  GCCCGGAGGG TGGCGGGCAG GACGCCCGCC ATAAACTGCC AGGCATCAAA 
   3351  TTAAGCAGAA GGCCATCCTG ACGGATGGCC TTTTTGCGTT TCTACAAACT 
   3401  CTTTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC 
   3451  AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT 
   3501  ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT 
   3551  TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG 
   3601  ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT 
   3651  AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTCCCAA TGATGAGCAC 
   3701  TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC 
   3751  AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG 
   3801  TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA 
   3851  ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC 
   3901  TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC 
   3951  ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA 
   4001  AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA 
   4051  CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG 
   4101  CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT 
   4151  GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG 
   4201  GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG 
   4251  CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA 
   4301  TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT 
   4351  GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTACCC 
   4401  CGGTTGATAA TCAGAAAAGC CCCAAAAACA GGAAGATTGT ATAAGCAAAT 
   4451  ATTTAAATTG TAAACGTTAA TATTTTGTTA AAATTCGCGT TAAATTTTTG 
   4501  TTAAATCAGC TCATTTTTTA ACCAATAGGC CGAAATCGGC AAAATCCCTT 
   4551  ATAAATCAAA AGAATAGACC GAGATAGGGT TGAGTGTTGT TCCAGTTTGG 
   4601  AACAAGAGTC CACTATTAAA GAACGTGGAC TCCAACGTCA AAGGGCGAAA 
   4651  AACCGTCTAT CAGGGCGATG GCCCACTACG TGAACCATCA CCCAAATCAA 
   4701  GTTTTTTGGG GTCGAGGTGC CGTAAAGCAC TAAATCGGAA CCCTAAAGGG 
   4751  AGCCCCCGAT TTAGAGCTTG ACGGGGAAAG CCGGCGAACG TGGCGAGAAA 
   4801  GGAAGGGAAG AAAGCGAAAG GAGCGGGCGC TAGGGCGCTG GCAAGTGTAG 
   4851  CGGTCACGCT GCGCGTAACC ACCACACCCG CCGCGCTTAA TGCGCCGCTA 
   4901  CAGGGCGCGT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA 
   4951  CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA 
   5001  GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG 
   5051  CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG 
   5101  ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG 
   5151  CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT 
   5201  CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC 
   5251  CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA 
   5301  AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC 
   5351  GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC 
   5401  TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG 
   5451  GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA 
   5501  GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC 
   5551  ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC 
   5601  CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG 
   5651  CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG 
   5701  ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA 
   5751  ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT 
   5801  GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG 
   5851  GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA 
   5901  CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC 
   5951  CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT 
   6001  TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC 
   6051  ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT 
   6101  GGTCGTGCAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG 
   6151  TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT 
   6201  GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG 
   6251  GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC 
   6301  ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA 
   6351  GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC 
   6401  AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT 
   6451  AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC 
   6501  TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC 
   6551  ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT 
   6601  CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA 
   6651  GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC 
   6701  AGGACCCAAC GCTGCCCGAA ATT
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