MG1655 seqA-mEOS3.2: Difference between revisions
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| Sources | | Sources | ||
| [https://bccm.belspo.be/ | | [https://bccm.belspo.be/page/genecorner-hosts-display-razor/661 LMBP 9777] | ||
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| References | | References | ||
Latest revision as of 11:31, 28 October 2024
| Name | MG1655 seqA-mEOS3.2 |
| Synonyms | |
| Genotype | F– λ– ilvG– rfb-50 rph-1 seqA-mEOS3.2:kmR |
| Phenotype | Kanamycin resistant |
| Sources | LMBP 9777 |
| References | PMID:26449690 |
Notes
The native seqA gene was fused at its 3' end to the fluorescent protein gene mEOS3.2, separated with a linker (ASPPPGRSR) and followed by a kanamycin resistance marker. The entire cassette was integrated in the chromosome.
This strain can be used to study the subcellular localization of the E. coli DNA binding protein SeqA.