PMID:21527672: Difference between revisions
imported>Wikientrybot change PMID template to TableEdit version |
imported>Wikientrybot change PMID template to TableEdit version |
||
| (One intermediate revision by the same user not shown) | |||
| Line 12: | Line 12: | ||
!align=left |Citation | !align=left |Citation | ||
|| | || | ||
''' | '''Saks, ME, Sanderson, LE, Choi, DS, Crosby, CM and Uhlenbeck, OC''' (2011) Functional consequences of T-stem mutations in E. coli tRNAThrUGU in vitro and in vivo. ''RNA'' '''17''':1038-47 | ||
|- | |- | ||
!align=left |Abstract | !align=left |Abstract | ||
| Line 38: | Line 38: | ||
==Annotations {{HelpIcon|Help:Literature}}== | ==Annotations {{HelpIcon|Help:Literature}}== | ||
<protect>< | <protect><annotationlinks/></protect> | ||
==EcoliWiki Links {{HelpIcon|Help:Literature}} == | ==EcoliWiki Links {{HelpIcon|Help:Literature}} == | ||
Latest revision as of 04:38, 15 September 2013
| Citation |
Saks, ME, Sanderson, LE, Choi, DS, Crosby, CM and Uhlenbeck, OC (2011) Functional consequences of T-stem mutations in E. coli tRNAThrUGU in vitro and in vivo. RNA 17:1038-47 |
|---|---|
| Abstract |
The binding affinities between Escherichia coli EF-Tu and 34 single and double base-pair changes in the T stem of E. coli tRNA(Thr)(UGU) were compared with similar data obtained previously for several aa-tRNAs binding to Thermus thermophilus EF-Tu. With a single exception, the two proteins bound to mutations in three T-stem base pairs in a quantitatively identical manner. However, tRNA(Thr) differs from other tRNAs by also using its rare A52-C62 pair as a negative specificity determinant. Using a plasmid-based tRNA gene replacement strategy, we show that many of the tRNA(Thr)(UGU) T-stem changes are either unable to support growth of E. coli or are less effective than the wild-type sequence. Since the inviable T-stem sequences are often present in other E. coli tRNAs, it appears that T-stem sequences in each tRNA body have evolved to optimize function in a different way. Although mutations of tRNA(Thr) can substantially increase or decrease its affinity to EF-Tu, the observed affinities do not correlate with the growth phenotype of the mutations in any simple way. This may either reflect the different conditions used in the two assays or indicate that the T-stem mutants affect another step in the translation mechanism. |
| Links |
PubMed PMC3096036 Online version:10.1261/rna.2427311 |
| Keywords |
Base Sequence; Escherichia coli/genetics; Escherichia coli/metabolism; Molecular Sequence Data; Mutation; Nucleic Acid Conformation; Peptide Elongation Factor Tu/chemistry; Peptide Elongation Factor Tu/metabolism; RNA, Bacterial/chemistry; RNA, Bacterial/metabolism; RNA, Transfer, Thr/chemistry; RNA, Transfer, Thr/metabolism; Thermus thermophilus/genetics; Thermus thermophilus/metabolism |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.