PMID:1438220: Difference between revisions

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'''Wickner, S, Skowyra, D, Hoskins, J and McKenney, K'''  (1992) DnaJ, DnaK, and GrpE heat shock proteins are required in oriP1 DNA replication solely at the RepA monomerization step.''Proc. Natl. Acad. Sci. U.S.A.'' '''89''':10345-9
'''Wickner, S, Skowyra, D, Hoskins, J and McKenney, K'''  (1992) DnaJ, DnaK, and GrpE heat shock proteins are required in oriP1 DNA replication solely at the RepA monomerization step. ''Proc. Natl. Acad. Sci. U.S.A.'' '''89''':10345-9
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1438220 PubMed]
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1438220 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC50335 PMC50335]


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Bacterial Proteins; DNA Helicases; DNA Replication; DNA, Bacterial; DNA-Binding Proteins; Escherichia coli; Escherichia coli Proteins; Genetic Complementation Test; HSP40 Heat-Shock Proteins; HSP70 Heat-Shock Proteins; Heat-Shock Proteins; Kinetics; Models, Genetic; Models, Structural; Proteins; Trans-Activators
Bacterial Proteins/metabolism; DNA Helicases; DNA Replication; DNA, Bacterial/genetics; DNA, Bacterial/metabolism; DNA-Binding Proteins/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; Genetic Complementation Test; HSP40 Heat-Shock Proteins; HSP70 Heat-Shock Proteins; Heat-Shock Proteins/metabolism; Kinetics; Models, Genetic; Models, Structural; Proteins; Trans-Activators


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Latest revision as of 04:21, 15 September 2013

Citation

Wickner, S, Skowyra, D, Hoskins, J and McKenney, K (1992) DnaJ, DnaK, and GrpE heat shock proteins are required in oriP1 DNA replication solely at the RepA monomerization step. Proc. Natl. Acad. Sci. U.S.A. 89:10345-9

Abstract

We have found that three Escherichia coli heat shock proteins, DnaK (the hsp70 homolog), DnaJ, and GrpE, function in oriP1 DNA replication in vitro solely to activate DNA binding by the replication initiator protein RepA. Activation results from the conversion of P1 or P7 RepA dimers to monomers that bind with high affinity to the origin of replication of plasmid P1. Thus, the essential role of these three heat shock proteins in this replication system is to change the quaternary structure of a single protein, RepA.

Links

PubMed PMC50335

Keywords

Bacterial Proteins/metabolism; DNA Helicases; DNA Replication; DNA, Bacterial/genetics; DNA, Bacterial/metabolism; DNA-Binding Proteins/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; Genetic Complementation Test; HSP40 Heat-Shock Proteins; HSP70 Heat-Shock Proteins; Heat-Shock Proteins/metabolism; Kinetics; Models, Genetic; Models, Structural; Proteins; Trans-Activators

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Annotations

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