PMID:326784: Difference between revisions
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'''Hogg, RW and Hermodson, MA''' (1977) Amino acid sequence of the L-arabinose-binding protein from Escherichia coli B/r.''J. Biol. Chem.'' '''252''':5135-41 | '''Hogg, RW and Hermodson, MA''' (1977) Amino acid sequence of the L-arabinose-binding protein from Escherichia coli B/r. ''J. Biol. Chem.'' '''252''':5135-41 | ||
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Amino Acid Sequence; Arabinose; Bacterial Proteins; Carrier Proteins; Escherichia coli; Peptide Fragments | Amino Acid Sequence; Arabinose/metabolism; Bacterial Proteins; Carrier Proteins/metabolism; Escherichia coli; Peptide Fragments | ||
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==Significance {{HelpIcon|Help:Literature}} == | ==Significance {{HelpIcon|Help:Literature}} == | ||
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Latest revision as of 04:46, 15 September 2013
| Citation |
Hogg, RW and Hermodson, MA (1977) Amino acid sequence of the L-arabinose-binding protein from Escherichia coli B/r. J. Biol. Chem. 252:5135-41 |
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| Abstract |
The amino acid sequence of the L-arabinose-binding protein of Escherichia coli B/r was determined by sequenator analyses of reduced and S-pyridylethylated L-arabinose-binding protein and fragments derived by chemical and enzymatic cleavage of the native protein. The fragments were the products of cleavage by cyanogen bromide. BNPS-skatole, hydroxylamine, mild acid hydrolysis, limited trypsin digestion, chymotrypsin subdigestion, and subdigestion with Staphylococcus aureus protease V8. The COOH-terminal sequence was determined using bovine carboxypeptidases A and B and amino acid analyses. The L-arabinose-binding protein was determined to contain 306 amino acid residues, the sequence of which is presented below. |
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| Keywords |
Amino Acid Sequence; Arabinose/metabolism; Bacterial Proteins; Carrier Proteins/metabolism; Escherichia coli; Peptide Fragments |
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