PMID:21402428: Difference between revisions

From EcoliWiki
Jump to navigation Jump to search
← Older edit
Fill PMID: Page!
imported>Wikientrybot
change PMID template to TableEdit version
 
(2 intermediate revisions by the same user not shown)
Line 1: Line 1:
{{RightTOC}}
{{RightTOC}}
<protect>
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.179597.H5223918b6e0c1-->
<span style = 'font-size: large'> '''Vidovic, S, Mangalappalli-Illathu, AK and Korber, DR'''  (2011) Prolonged cold stress response of Escherichia coli O157 and the role of rpoS.''Int. J. Food Microbiol.'' '''146''':163-9</span>
<!--
******************************************************************************************
*
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **
*
****************************************************************************************** -->
{|  id="H5223918b6e0c1"  class=" tableEdit PMID_info_table" 


{|{{Prettytable}} align = 'center' width= '70%'
|-
|-
|{{TableHeadingColor}} |'''Abstract''' || <protect>Phenotypic analyses were performed using an enterohemorrhagic Escherichia coli O157 (EHEC) strain (B-1) and a commensal E. coli K-12 strain, exposed to prolonged cold stress. The EHEC E. coli O157 showed significantly (P<0.05) higher resistance to cold stress compared to non-pathogenic E. coli K-12 DH5α. Further, it was found that RpoS sigma factor plays a significant (P<0.05) role in the cold stress physiology of the enterohemorrhagic E. coli strain. Using comparative proteomic analysis of hypo-thermally adapted E. coli O157 wild-type and rpoS mutant strains, we identified 21 proteins that were differentially expressed upon cold temperature shifts or rpoS mutation. All identified proteins of cold post-acclimation stimulons fell into two large sub-groups: (i) stress proteins, and (ii) housekeeping proteins. This prolonged cold stress response included proteins involved in mRNA turnover, cell replication efficiency, conditional and post-synthetic modification of membrane lipid bilayers, biosynthetic processes, and the uptake of different sugars. The RpoS sigma factor had no control over the key stress proteins, polynucleotide phosphorylase and elongation factor G, in prolonged stress stimulon. However, RpoS was shown to regulate the expression of proteins involved in homeoviscous adaptation during cold shock, as well as various proteins involved in central metabolic pathways of this food-borne pathogen.</protect>
!align=left  |Citation
||
'''Vidovic, S, Mangalappalli-Illathu, AK and Korber, DR''' (2011) Prolonged cold stress response of Escherichia coli O157 and the role of rpoS. ''Int. J. Food Microbiol.'' '''146''':163-9
|-
|-
|{{TableHeadingColor}} nowrap |'''Links''' ||
!align=left  |Abstract
*  [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=21402428 PubMed]
||
Phenotypic analyses were performed using an enterohemorrhagic Escherichia coli O157 (EHEC) strain (B-1) and a commensal E. coli K-12 strain, exposed to prolonged cold stress. The EHEC E. coli O157 showed significantly (P<0.05) higher resistance to cold stress compared to non-pathogenic E. coli K-12 DH5α. Further, it was found that RpoS sigma factor plays a significant (P<0.05) role in the cold stress physiology of the enterohemorrhagic E. coli strain. Using comparative proteomic analysis of hypo-thermally adapted E. coli O157 wild-type and rpoS mutant strains, we identified 21 proteins that were differentially expressed upon cold temperature shifts or rpoS mutation. All identified proteins of cold post-acclimation stimulons fell into two large sub-groups: (i) stress proteins, and (ii) housekeeping proteins. This prolonged cold stress response included proteins involved in mRNA turnover, cell replication efficiency, conditional and post-synthetic modification of membrane lipid bilayers, biosynthetic processes, and the uptake of different sugars. The RpoS sigma factor had no control over the key stress proteins, polynucleotide phosphorylase and elongation factor G, in prolonged stress stimulon. However, RpoS was shown to regulate the expression of proteins involved in homeoviscous adaptation during cold shock, as well as various proteins involved in central metabolic pathways of this food-borne pathogen.
|-
!align=left  |Links
||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=21402428 PubMed]
Online version:[http://dx.doi.org/10.1016/j.ijfoodmicro.2011.02.018 10.1016/j.ijfoodmicro.2011.02.018]
Online version:[http://dx.doi.org/10.1016/j.ijfoodmicro.2011.02.018 10.1016/j.ijfoodmicro.2011.02.018]
|-
!align=left  |Keywords
||
Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Carbohydrate Metabolism; Cell Membrane/metabolism; Cold Temperature; Cold-Shock Response; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli/physiology; Escherichia coli O157/genetics; Escherichia coli O157/metabolism; Escherichia coli O157/physiology; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Mutation; Proteome/analysis; RNA, Bacterial/metabolism; RNA, Messenger/metabolism; Sigma Factor/genetics; Sigma Factor/metabolism
|- class="tableEdit_footer"
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.179597.H5223918b6e0c1&page=179597&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table]</span> ||
|}
|}
</protect>
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.179597.H5223918b6e0c1-->
==Significance {{HelpIcon|Help:Literature}} ==
==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}
{{LitSignificance}}
Line 19: Line 38:


==Annotations {{HelpIcon|Help:Literature}}==
==Annotations {{HelpIcon|Help:Literature}}==
<protect><!--box uid=63ce1649aab8b3180235b0af7618f667.179597.R4e41c6dc57c71-->
<protect><annotationlinks/></protect>
<!--
******************************************************************************************
*
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **
*
****************************************************************************************** -->
{|  id="R4e41c6dc57c71"  class=" tableEdit GO_table_reference dataTable" 
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
 
|- class="tableEdit_footer"
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.179597.R4e41c6dc57c71&page=179597&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table]</span> || || || || || || || ||
|}
<!--box uid=63ce1649aab8b3180235b0af7618f667.179597.R4e41c6dc57c71--></protect>


==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
==EcoliWiki Links {{HelpIcon|Help:Literature}} ==

Latest revision as of 04:38, 15 September 2013

Citation

Vidovic, S, Mangalappalli-Illathu, AK and Korber, DR (2011) Prolonged cold stress response of Escherichia coli O157 and the role of rpoS. Int. J. Food Microbiol. 146:163-9

Abstract

Phenotypic analyses were performed using an enterohemorrhagic Escherichia coli O157 (EHEC) strain (B-1) and a commensal E. coli K-12 strain, exposed to prolonged cold stress. The EHEC E. coli O157 showed significantly (P<0.05) higher resistance to cold stress compared to non-pathogenic E. coli K-12 DH5α. Further, it was found that RpoS sigma factor plays a significant (P<0.05) role in the cold stress physiology of the enterohemorrhagic E. coli strain. Using comparative proteomic analysis of hypo-thermally adapted E. coli O157 wild-type and rpoS mutant strains, we identified 21 proteins that were differentially expressed upon cold temperature shifts or rpoS mutation. All identified proteins of cold post-acclimation stimulons fell into two large sub-groups: (i) stress proteins, and (ii) housekeeping proteins. This prolonged cold stress response included proteins involved in mRNA turnover, cell replication efficiency, conditional and post-synthetic modification of membrane lipid bilayers, biosynthetic processes, and the uptake of different sugars. The RpoS sigma factor had no control over the key stress proteins, polynucleotide phosphorylase and elongation factor G, in prolonged stress stimulon. However, RpoS was shown to regulate the expression of proteins involved in homeoviscous adaptation during cold shock, as well as various proteins involved in central metabolic pathways of this food-borne pathogen.

Links

PubMed Online version:10.1016/j.ijfoodmicro.2011.02.018

Keywords

Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Carbohydrate Metabolism; Cell Membrane/metabolism; Cold Temperature; Cold-Shock Response; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli/physiology; Escherichia coli O157/genetics; Escherichia coli O157/metabolism; Escherichia coli O157/physiology; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Mutation; Proteome/analysis; RNA, Bacterial/metabolism; RNA, Messenger/metabolism; Sigma Factor/genetics; Sigma Factor/metabolism

edit table

Significance

You can help EcoliWiki by summarizing why this paper is useful

Useful Materials and Methods

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Annotations

<annotationlinks/>

EcoliWiki Links

Add links to pages that link here (e.g. gene, product, method pages)

See also

References

See Help:References for how to manage references in EcoliWiki.

Retrieved from "https://ecoliwiki.org/colipedia/index.php?title=PMID:21402428&oldid=1464977"