Quinolones: Difference between revisions
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== | ==Targets== | ||
Type II Topoisomerases | |||
*DNA Gyrase | |||
*Topoisomerase IV<ref name='PMID:8524852'/> | |||
**inhibition by norfloxacin reduces activity to a rate of 1/100 the normal level<ref name='PMID:7590259'/> | |||
Results in replication fork collapse and induction of the SOS response due to permanent double-stranded breaks caused by the formation of poisonous protein:DNA adducts. | |||
==Resistant Alleles== | |||
*ParC | |||
==Usage Examples and Notes== | ==Usage Examples and Notes== | ||
*Norfloxacin<ref name='PMID:16164551'/> | *Norfloxacin<ref name='PMID:16164551'/> | ||
*Oxolinic acid and nalidixic acid interact with the same target to inhibit replication DNA synthesis. Although nalidixic acid is used extensively, oxolinic acid has ben found to be 10-fold more active of an inhibitor. Mutants with resistance to one drug also show resistance to the other drug. <ref name='PMID:770163'/> | |||
== References == | == References == | ||
Latest revision as of 16:04, 1 October 2013
Targets
Type II Topoisomerases
- DNA Gyrase
- Topoisomerase IV[1]
- inhibition by norfloxacin reduces activity to a rate of 1/100 the normal level[2]
Results in replication fork collapse and induction of the SOS response due to permanent double-stranded breaks caused by the formation of poisonous protein:DNA adducts.
Resistant Alleles
- ParC
Usage Examples and Notes
- Norfloxacin[3]
- Oxolinic acid and nalidixic acid interact with the same target to inhibit replication DNA synthesis. Although nalidixic acid is used extensively, oxolinic acid has ben found to be 10-fold more active of an inhibitor. Mutants with resistance to one drug also show resistance to the other drug. [4]
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Khodursky, AB et al. (1995) Topoisomerase IV is a target of quinolones in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 92 11801-5 PubMed
- ↑ Zechiedrich, EL & Cozzarelli, NR (1995) Roles of topoisomerase IV and DNA gyrase in DNA unlinking during replication in Escherichia coli. Genes Dev. 9 2859-69 PubMed
- ↑ Lopez, CR et al. (2005) A role for topoisomerase III in a recombination pathway alternative to RuvABC. Mol. Microbiol. 58 80-101 PubMed
- ↑ Staudenbauer, WL (1976) Replication of Escherichia coli DNA in vitro: inhibition by oxolinic acid. Eur. J. Biochem. 62 491-7 PubMed