yhdJ:Quickview: Difference between revisions

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{{Tab On|Quickview|yhdJ}}{{Tab|Gene|yhdJ}}{{Tab|Gene Product(s)|yhdJ}}{{Tab|Expression|yhdJ}}{{Tab|Evolution|yhdJ}}{{Tab|On One Page|yhdJ}}{{TabsBottom}}
{{Tab On|Quickview|yhdJ}}{{Tab|Gene|yhdJ}}{{Tab|Gene Product(s)|yhdJ}}{{Tab|Expression|yhdJ}}{{Tab|Evolution|yhdJ}}{{Tab|On One Page|yhdJ}}{{TabsBottom}}
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!{{table_heading_style}} |Gene Name
!align=left {{table_heading_style}} |Standard Name
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[[yhdJ:Gene|''yhdJ]]'' (synonyms: ''ECK3249, b3262, JW5543, ccrM'')<ref name='LIB:Riley_2006'/>
''yhdJ''
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!{{table_heading_style}} |Product(s)  
!align=left {{table_heading_style}} |Gene Synonym(s)
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[[yhdJ:Gene_Product(s)|YhdJ product]]<ref name='LIB:Riley_2006'/>: predicted methyltransferase<ref name='LIB:Riley_2006'/>, B3262<ref name='LIB:EcoCyc10.6'/>, YhdJ<ref name='LIB:EcoCyc10.6'/>, CcrM<ref name='LIB:EcoCyc10.6'/>
''ECK3249, b3262, JW5543, ccrM''<ref name='LIB:Riley_2006'/>, ccrM''
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!{{table_heading_style}} |Description
!align=left {{table_heading_style}} |Product Desc.
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predicted methyltransferase<ref name='LIB:EcoCyc10.6'/>DNA adenine methyltransferase<ref name='LIB:EcoCyc11.1'/>
predicted methyltransferase<ref name='LIB:EcoCyc10.6'/>; DNA adenine methyltransferase<ref name='LIB:EcoCyc11.1'/>
 


DNA adenine methyltransferase, SAM-dependent<ref name='LIB:EcoGene'/>
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!align=left {{table_heading_style}} |Product Synonyms(s)
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''predicted methyltransferase<ref name='LIB:Riley_2006'/>, B3262<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, YhdJ<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, CcrM<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/> , ccrM, ECK3249, JW5543, b3262''
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!align=left {{table_heading_style}} |Function from GO
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<GO_nr />
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!{{table_heading_style}} |Knock-Out Phenotype
!align=left {{table_heading_style}} |Knock-Out Phenotype
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!{{table_heading_style}} |Regulation/Expression
!align=left {{table_heading_style}} |Regulation/Expression
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transcription unit(s): [[:Category:txn_units:yhdJ|yhdJ]]<ref name='LIB:EcoCyc10.6'/>  
transcription unit(s): [[:Category:Txn_unit:yhdJ|yhdJ]]<ref name='LIB:EcoCyc10.6'/>  
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!{{table_heading_style}} |Regulation/Activity
!align=left {{table_heading_style}} |Regulation/Activity
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[[File:porteco.png|link=http://porteco.org/AjaxSearch.jsp?searchString=yhdJ&s=search]] [[File:EcoCyc.gif|link=http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG11498]] [[File:regulondb.jpg|link=http://regulondb.ccg.unam.mx/gene?term=ECK120001454&organism=ECK12&format=jsp&type=gene ]] <br/><br/> [[File:dnadisplay.png|link=http://ecoliwiki.net/tools/display_dna.php?gene=yhdJ&loc=MG1655%3A3409675..3410559,W3110%3A3411508..3412392,REL606%3A3339544..3340428,DH10B%3A3507420..3508304,BW2952%3A3296823..3297707]] [[File:proteindisplay.png|link=http://ecoliwiki.net/tools/display_protein.php?f=NC_000913&start=3296823&end=3297707&gene=yhdJ&aa=mtmrtgceptrfgneaktiihgdalaelkkipaesvdlifadppynigknfdglieawkedlfidwlfeviaechrvlkkqgsmyimnstenmpfidlqcrklftiksrivwsydssgvqakkhygsmyepilmmvkdaknytfngdailveaktgsqralidyrknppqpynhqkvpgnvwdfprvrylmdeyenhptqkpeallkriilassnpgdivldpfagsfttgavaiasgrkfigieinseyikmglrrldvashysaeelakvkkrktgnlskrsrlsevdpdlitk]]&nbsp;&nbsp;  [[File:pubmed.jpg|link=http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=Search&Term=yhdJ+AND+escherichia+coli]]&nbsp;&nbsp;  [[File:textpresso.jpg|link=http://textpresso.tamu.edu/cgi-bin/textpresso/search?q=yhdJ]]&nbsp;&nbsp;
 
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==Notes==
===Notes===
{{BacktoTop}}
 
In vitro DNA adenine methyltransferase activity has been demonstrated for YhdJ; overexpression of YhdJ leads to protection of chromosomal DNA from NsiI digestion, but DNA from wild type cells is not protected (Broadbent (2007). DNA from a DNA methyltransferase deficient strain (dam-, dcm-, EcoK-) does not react with anti-N6-methylA or anti-N4-methylC antibodies when YhdJ is expressed from the pBR322 Tet promoter; the yhdJ-containing pBR322 derivative was not resistant to a variety of restriction (personal communication: R. Vaisvila, S. Stickel and R.J. Roberts, unpublished results, April, 2006). The Kossykh (2004) report describing yhdJ as ccrM, an adenine methyltransferase, has been retracted.<ref name='LIB:EcoGene'/>
 




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==References==
==References==
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Latest revision as of 21:41, 27 May 2013

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
References Suggestions
Standard Name

yhdJ

Gene Synonym(s)

ECK3249, b3262, JW5543, ccrM[1], ccrM

Product Desc.

predicted methyltransferase[2]; DNA adenine methyltransferase[3]

DNA adenine methyltransferase, SAM-dependent[4]

Product Synonyms(s)

predicted methyltransferase[1], B3262[2][1], YhdJ[2][1], CcrM[2][1] , ccrM, ECK3249, JW5543, b3262

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): yhdJ[2]

Regulation/Activity
Quick Links



        

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Notes

[back to top]


In vitro DNA adenine methyltransferase activity has been demonstrated for YhdJ; overexpression of YhdJ leads to protection of chromosomal DNA from NsiI digestion, but DNA from wild type cells is not protected (Broadbent (2007). DNA from a DNA methyltransferase deficient strain (dam-, dcm-, EcoK-) does not react with anti-N6-methylA or anti-N4-methylC antibodies when YhdJ is expressed from the pBR322 Tet promoter; the yhdJ-containing pBR322 derivative was not resistant to a variety of restriction (personal communication: R. Vaisvila, S. Stickel and R.J. Roberts, unpublished results, April, 2006). The Kossykh (2004) report describing yhdJ as ccrM, an adenine methyltransferase, has been retracted.[4]



References

[back to top]


  1. ↑ 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
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