PMID:2017367: Difference between revisions

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<span style = 'font-size: large'> '''Welsh, J and McClelland, M'''  (1991) Genomic fingerprints produced by PCR with consensus tRNA gene primers.''Nucleic Acids Res.'' '''19''':861-6</span>
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!align=left  |Citation
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'''Welsh, J and McClelland, M'''  (1991) Genomic fingerprints produced by PCR with consensus tRNA gene primers. ''Nucleic Acids Res.'' '''19''':861-6
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!align=left  |Abstract
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The polymerase chain reaction using only a single 'consensus' tRNA gene primer, or a pair of primers facing outward from tRNA genes, amplifies a set of DNA fragments in bacterial, plant and animal genomic DNAs. Presumably, these PCR fingerprints are mainly derived from the regions between closely linked tRNA genes. The pattern of the PCR products is determined by which genomes and which primer(s) are used. Genomic fingerprints are largely conserved within a species and, in bacteria, most products in the fingerprint are conserved between closely related species. Thus, PCR with tRNA gene consensus primers helps to identify species and genera.
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!align=left  |Links
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2017367 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC333722 PMC333722]


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|{{TableHeadingColor}} |'''Abstract''' || The polymerase chain reaction using only a single 'consensus' tRNA gene primer, or a pair of primers facing outward from tRNA genes, amplifies a set of DNA fragments in bacterial, plant and animal genomic DNAs. Presumably, these PCR fingerprints are mainly derived from the regions between closely linked tRNA genes. The pattern of the PCR products is determined by which genomes and which primer(s) are used. Genomic fingerprints are largely conserved within a species and, in bacteria, most products in the fingerprint are conserved between closely related species. Thus, PCR with tRNA gene consensus primers helps to identify species and genera.
!align=left  |Keywords
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Base Sequence; DNA Fingerprinting; DNA, Bacterial/genetics; Genes, Bacterial; Molecular Sequence Data; Polymerase Chain Reaction; RNA Probes; RNA, Transfer/genetics; Staphylococcus/genetics; Streptococcus/genetics
|{{TableHeadingColor}} nowrap |'''Links''' ||  [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2017367 PubMed]
 
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==Annotations {{HelpIcon|Help:Literature}}==
==Annotations {{HelpIcon|Help:Literature}}==
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!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
 
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==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
==EcoliWiki Links {{HelpIcon|Help:Literature}} ==

Latest revision as of 04:36, 15 September 2013

Citation

Welsh, J and McClelland, M (1991) Genomic fingerprints produced by PCR with consensus tRNA gene primers. Nucleic Acids Res. 19:861-6

Abstract

The polymerase chain reaction using only a single 'consensus' tRNA gene primer, or a pair of primers facing outward from tRNA genes, amplifies a set of DNA fragments in bacterial, plant and animal genomic DNAs. Presumably, these PCR fingerprints are mainly derived from the regions between closely linked tRNA genes. The pattern of the PCR products is determined by which genomes and which primer(s) are used. Genomic fingerprints are largely conserved within a species and, in bacteria, most products in the fingerprint are conserved between closely related species. Thus, PCR with tRNA gene consensus primers helps to identify species and genera.

Links

PubMed PMC333722

Keywords

Base Sequence; DNA Fingerprinting; DNA, Bacterial/genetics; Genes, Bacterial; Molecular Sequence Data; Polymerase Chain Reaction; RNA Probes; RNA, Transfer/genetics; Staphylococcus/genetics; Streptococcus/genetics

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