PMID:19767843: Difference between revisions

From EcoliWiki
Jump to navigation Jump to search
← Older edit
/Volumes/r3/t3/ul/working/touchTables/touch_all_tables.php
imported>Wikientrybot
change PMID template to TableEdit version
 
(2 intermediate revisions by the same user not shown)
Line 1: Line 1:
{{RightTOC}}
{{RightTOC}}
<protect>
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.170018.O5223906adceb0-->
<span style = 'font-size: large'> '''Moen, B, Janbu, AO, Langsrud, S, Langsrud, O, Hobman, JL, Constantinidou, C, Kohler, A and Rudi, K'''  (2009) Global responses of Escherichia coli to adverse conditions determined by microarrays and FT-IR spectroscopy.''Can. J. Microbiol.'' '''55''':714-28</span>
<!--
******************************************************************************************
*
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **
*
****************************************************************************************** -->
{|  id="O5223906adceb0"  class=" tableEdit PMID_info_table" 


{|{{Prettytable}} align = 'center' width= '70%'
|-
|-
|{{TableHeadingColor}} |'''Abstract''' || <protect>The global gene expression and biomolecular composition in an Escherichia coli model strain exposed to 10 adverse conditions (sodium chloride, ethanol, glycerol, hydrochloric and acetic acid, sodium hydroxide, heat (46 degrees C), and cold (15 degrees C), as well as ethidium bromide and the disinfectant benzalkonium chloride) were determined using DNA microarrays and Fourier transform infrared (FT-IR) spectroscopy. In total, approximately 40% of all investigated genes (1682/4279 genes) significantly changed expression, compared with a nonstressed control. There were, however, only 3 genes (ygaW (unknown function), rmf (encoding a ribosomal modification factor), and ghrA (encoding a glyoxylate/hydroxypyruvate reductase)) that significantly changed expression under all conditions (not including benzalkonium chloride). The FT-IR analysis showed an increase in unsaturated fatty acids during ethanol and cold exposure, and a decrease during acid and heat exposure. Cold conditions induced changes in the carbohydrate composition of the cell, possibly related to the upregulation of outer membrane genes (glgAP and rcsA). Although some covariance was observed between the 2 data sets, principle component analysis and regression analyses revealed that the gene expression and the biomolecular responses are not well correlated in stressed populations of E. coli, underlining the importance of multiple strategies to begin to understand the effect on the whole cell.</protect>
!align=left  |Citation
||
'''Moen, B, Janbu, AO, Langsrud, S, Langsrud, O, Hobman, JL, Constantinidou, C, Kohler, A and Rudi, K'''  (2009) Global responses of Escherichia coli to adverse conditions determined by microarrays and FT-IR spectroscopy. ''Can. J. Microbiol.'' '''55''':714-28
|-
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=19767843 PubMed]<br/>Online version:[http://dx.doi.org/10.1139/w09-016 10.1139/w09-016]
!align=left  |Abstract
||
The global gene expression and biomolecular composition in an Escherichia coli model strain exposed to 10 adverse conditions (sodium chloride, ethanol, glycerol, hydrochloric and acetic acid, sodium hydroxide, heat (46 degrees C), and cold (15 degrees C), as well as ethidium bromide and the disinfectant benzalkonium chloride) were determined using DNA microarrays and Fourier transform infrared (FT-IR) spectroscopy. In total, approximately 40% of all investigated genes (1682/4279 genes) significantly changed expression, compared with a nonstressed control. There were, however, only 3 genes (ygaW (unknown function), rmf (encoding a ribosomal modification factor), and ghrA (encoding a glyoxylate/hydroxypyruvate reductase)) that significantly changed expression under all conditions (not including benzalkonium chloride). The FT-IR analysis showed an increase in unsaturated fatty acids during ethanol and cold exposure, and a decrease during acid and heat exposure. Cold conditions induced changes in the carbohydrate composition of the cell, possibly related to the upregulation of outer membrane genes (glgAP and rcsA). Although some covariance was observed between the 2 data sets, principle component analysis and regression analyses revealed that the gene expression and the biomolecular responses are not well correlated in stressed populations of E. coli, underlining the importance of multiple strategies to begin to understand the effect on the whole cell.
|-
!align=left  |Links
||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=19767843 PubMed]
Online version:[http://dx.doi.org/10.1139/w09-016 10.1139/w09-016]
|-
!align=left  |Keywords
||
Bacterial Proteins/genetics; Escherichia coli/chemistry; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli/metabolism; Fatty Acids/metabolism; Gene Expression Regulation, Bacterial; Oligonucleotide Array Sequence Analysis; Organic Chemicals/pharmacology; Spectroscopy, Fourier Transform Infrared; Temperature
 
|- class="tableEdit_footer"
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.170018.O5223906adceb0&page=170018&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table]</span> ||
|}
|}
</protect>
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.170018.O5223906adceb0-->
==Significance {{HelpIcon|Help:Literature}} ==
==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}
{{LitSignificance}}
Line 17: Line 38:


==Annotations {{HelpIcon|Help:Literature}}==
==Annotations {{HelpIcon|Help:Literature}}==
<protect><!--box uid=63ce1649aab8b3180235b0af7618f667.170018.C4b269ff6cff3a-->
<protect><annotationlinks/></protect>
<!--
******************************************************************************************
*
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **
*
****************************************************************************************** -->
{|  id="C4b269ff6cff3a"  class=" tableEdit GO_table_reference" 
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
 
|- class="tableEdit_footer"
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.170018.C4b269ff6cff3a&page=170018&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table]</span> || || || || || || || ||
|}
<!--box uid=63ce1649aab8b3180235b0af7618f667.170018.C4b269ff6cff3a--></protect>


==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
==EcoliWiki Links {{HelpIcon|Help:Literature}} ==

Latest revision as of 04:35, 15 September 2013

Citation

Moen, B, Janbu, AO, Langsrud, S, Langsrud, O, Hobman, JL, Constantinidou, C, Kohler, A and Rudi, K (2009) Global responses of Escherichia coli to adverse conditions determined by microarrays and FT-IR spectroscopy. Can. J. Microbiol. 55:714-28

Abstract

The global gene expression and biomolecular composition in an Escherichia coli model strain exposed to 10 adverse conditions (sodium chloride, ethanol, glycerol, hydrochloric and acetic acid, sodium hydroxide, heat (46 degrees C), and cold (15 degrees C), as well as ethidium bromide and the disinfectant benzalkonium chloride) were determined using DNA microarrays and Fourier transform infrared (FT-IR) spectroscopy. In total, approximately 40% of all investigated genes (1682/4279 genes) significantly changed expression, compared with a nonstressed control. There were, however, only 3 genes (ygaW (unknown function), rmf (encoding a ribosomal modification factor), and ghrA (encoding a glyoxylate/hydroxypyruvate reductase)) that significantly changed expression under all conditions (not including benzalkonium chloride). The FT-IR analysis showed an increase in unsaturated fatty acids during ethanol and cold exposure, and a decrease during acid and heat exposure. Cold conditions induced changes in the carbohydrate composition of the cell, possibly related to the upregulation of outer membrane genes (glgAP and rcsA). Although some covariance was observed between the 2 data sets, principle component analysis and regression analyses revealed that the gene expression and the biomolecular responses are not well correlated in stressed populations of E. coli, underlining the importance of multiple strategies to begin to understand the effect on the whole cell.

Links

PubMed Online version:10.1139/w09-016

Keywords

Bacterial Proteins/genetics; Escherichia coli/chemistry; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli/metabolism; Fatty Acids/metabolism; Gene Expression Regulation, Bacterial; Oligonucleotide Array Sequence Analysis; Organic Chemicals/pharmacology; Spectroscopy, Fourier Transform Infrared; Temperature

edit table

Significance

You can help EcoliWiki by summarizing why this paper is useful

Useful Materials and Methods

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Annotations

<annotationlinks/>

EcoliWiki Links

Add links to pages that link here (e.g. gene, product, method pages)

See also

References

See Help:References for how to manage references in EcoliWiki.

Retrieved from "https://ecoliwiki.org/colipedia/index.php?title=PMID:19767843&oldid=1464020"