PMID:10074062: Difference between revisions

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<span style = 'font-size: large'> '''Zeng, X and Saxild, HH'''  (1999) Identification and characterization of a DeoR-specific operator sequence essential for induction of dra-nupC-pdp operon expression in Bacillus subtilis.''J. Bacteriol.'' '''181''':1719-27</span>
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!align=left  |Citation
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'''Zeng, X and Saxild, HH'''  (1999) Identification and characterization of a DeoR-specific operator sequence essential for induction of dra-nupC-pdp operon expression in Bacillus subtilis. ''J. Bacteriol.'' '''181''':1719-27
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!align=left  |Abstract
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The deoR gene located just upstream the dra-nupC-pdp operon of Bacillus subtilis encodes the DeoR repressor protein that negatively regulates the expression of the operon at the level of transcription. The control region upstream of the operon was mapped by the use of transcriptional lacZ fusions. It was shown that all of the cis-acting elements, which were necessary for full DeoR regulation of the operon, were included in a 141-bp sequence just upstream of dra. The increased copy number of this control region resulted in titration of the DeoR molecules of the cell. By using mutagenic PCR and site-directed mutagenesis techniques, a palindromic sequence located from position -60 to position -43 relative to the transcription start point was identified as a part of the operator site for the binding of DeoR. Furthermore, it was shown that a direct repeat of five nucleotides, which was identical to the 3' half of the palindrome and was located between the -10 and -35 regions of the dra promoter, might function as a half binding site involved in cooperative binding of DeoR to the regulatory region. Binding of DeoR protein to the operator DNA was confirmed by a gel electrophoresis mobility shift assay. Moreover, deoxyribose-5-phosphate was shown to be a likely candidate for the true inducer of the dra-nupC-pdp expression.
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!align=left  |Links
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10074062 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC93568 PMC93568]


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|{{TableHeadingColor}} |'''Abstract''' || The deoR gene located just upstream the dra-nupC-pdp operon of Bacillus subtilis encodes the DeoR repressor protein that negatively regulates the expression of the operon at the level of transcription. The control region upstream of the operon was mapped by the use of transcriptional lacZ fusions. It was shown that all of the cis-acting elements, which were necessary for full DeoR regulation of the operon, were included in a 141-bp sequence just upstream of dra. The increased copy number of this control region resulted in titration of the DeoR molecules of the cell. By using mutagenic PCR and site-directed mutagenesis techniques, a palindromic sequence located from position -60 to position -43 relative to the transcription start point was identified as a part of the operator site for the binding of DeoR. Furthermore, it was shown that a direct repeat of five nucleotides, which was identical to the 3' half of the palindrome and was located between the -10 and -35 regions of the dra promoter, might function as a half binding site involved in cooperative binding of DeoR to the regulatory region. Binding of DeoR protein to the operator DNA was confirmed by a gel electrophoresis mobility shift assay. Moreover, deoxyribose-5-phosphate was shown to be a likely candidate for the true inducer of the dra-nupC-pdp expression.
!align=left  |Keywords
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Aldehyde-Lyases/genetics; Bacillus subtilis/genetics; Bacillus subtilis/metabolism; Bacterial Proteins/genetics; Base Sequence; Binding Sites/genetics; Carrier Proteins/genetics; DNA, Bacterial/genetics; DNA, Bacterial/metabolism; DNA-Binding Proteins; Gene Expression; Genes, Bacterial; Lac Operon; Membrane Transport Proteins; Molecular Sequence Data; Mutagenesis; Mutagenesis, Site-Directed; Operator Regions, Genetic/drug effects; Operon; Pentosyltransferases/genetics; Polymerase Chain Reaction; Pyrimidine Phosphorylases; Repressor Proteins/genetics; Ribosemonophosphates/metabolism; Ribosemonophosphates/pharmacology; Sequence Deletion
|{{TableHeadingColor}} nowrap |'''Links''' ||  [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10074062 PubMed]
 
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==Significance {{HelpIcon|Help:Literature}} ==
==Significance {{HelpIcon|Help:Literature}} ==
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==Annotations {{HelpIcon|Help:Literature}}==
==Annotations {{HelpIcon|Help:Literature}}==
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!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
 
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==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
==EcoliWiki Links {{HelpIcon|Help:Literature}} ==

Latest revision as of 04:09, 15 September 2013

Citation

Zeng, X and Saxild, HH (1999) Identification and characterization of a DeoR-specific operator sequence essential for induction of dra-nupC-pdp operon expression in Bacillus subtilis. J. Bacteriol. 181:1719-27

Abstract

The deoR gene located just upstream the dra-nupC-pdp operon of Bacillus subtilis encodes the DeoR repressor protein that negatively regulates the expression of the operon at the level of transcription. The control region upstream of the operon was mapped by the use of transcriptional lacZ fusions. It was shown that all of the cis-acting elements, which were necessary for full DeoR regulation of the operon, were included in a 141-bp sequence just upstream of dra. The increased copy number of this control region resulted in titration of the DeoR molecules of the cell. By using mutagenic PCR and site-directed mutagenesis techniques, a palindromic sequence located from position -60 to position -43 relative to the transcription start point was identified as a part of the operator site for the binding of DeoR. Furthermore, it was shown that a direct repeat of five nucleotides, which was identical to the 3' half of the palindrome and was located between the -10 and -35 regions of the dra promoter, might function as a half binding site involved in cooperative binding of DeoR to the regulatory region. Binding of DeoR protein to the operator DNA was confirmed by a gel electrophoresis mobility shift assay. Moreover, deoxyribose-5-phosphate was shown to be a likely candidate for the true inducer of the dra-nupC-pdp expression.

Links

PubMed PMC93568

Keywords

Aldehyde-Lyases/genetics; Bacillus subtilis/genetics; Bacillus subtilis/metabolism; Bacterial Proteins/genetics; Base Sequence; Binding Sites/genetics; Carrier Proteins/genetics; DNA, Bacterial/genetics; DNA, Bacterial/metabolism; DNA-Binding Proteins; Gene Expression; Genes, Bacterial; Lac Operon; Membrane Transport Proteins; Molecular Sequence Data; Mutagenesis; Mutagenesis, Site-Directed; Operator Regions, Genetic/drug effects; Operon; Pentosyltransferases/genetics; Polymerase Chain Reaction; Pyrimidine Phosphorylases; Repressor Proteins/genetics; Ribosemonophosphates/metabolism; Ribosemonophosphates/pharmacology; Sequence Deletion

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