PMID:816772: Difference between revisions
Fill PMID: Page! |
imported>Wikientrybot change PMID template to TableEdit version |
||
| (2 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
{{RightTOC}} | {{RightTOC}} | ||
< | <!--box uid=d41d8cd98f00b204e9800998ecf8427e.170389.U5223990075574--> | ||
< | <!-- | ||
****************************************************************************************** | |||
* | |||
* ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** | |||
* | |||
****************************************************************************************** --> | |||
{| id="U5223990075574" class=" tableEdit PMID_info_table" | |||
|- | |- | ||
| | !align=left |Citation | ||
|| | |||
'''Halegoua, S, Hirashima, A and Inouye, M''' (1976) Puromycin-resistant biosynthesis of a specific outer-membrane lipoprotein of Escherichia coli. ''J. Bacteriol.'' '''126''':183-91 | |||
|- | |- | ||
| | !align=left |Abstract | ||
|| | |||
The reported puromycin resistance of the in vivo biosynthesis of a specific outer-membrane lipoprotein of Escherichia coli was further investigated. The biosynthetic machinery making the lipoprotein was made more accessible to puromycin by disruption of the cell structure using ethylenediaminetetracetate or toluene, and finally in an in vitro protein biosynthesis system using polyribosomes. Puromycin sensitivity of overall protein synthesis increased by about 10-fold for each method of disruption of the cell structure; 50% inhibitions were obtained at 330, 35, 2.7, and 0.22 mug of puromycin per ml for intact cells, ethylenediaminetetraacetate-treated cells, toluene-treated cells, and the polyribosome system, respectively. However, the lipoprotein biosynthesis remained more resistant to puromycin than the biosynthesis of other proteins in all systems tested. These results strongly suggest that puromycin resistance of the lipoprotein biosynthesis is due to an intrinsic property of the lipoprotein biosynthetic machinery. | |||
|- | |||
!align=left |Links | |||
|| | |||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=816772 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC233273 PMC233273] | |||
|- | |||
!align=left |Keywords | |||
|| | |||
Bacterial Proteins/biosynthesis; Cell Wall/metabolism; Cell-Free System; Edetic Acid/pharmacology; Escherichia coli/drug effects; Escherichia coli/metabolism; Lipoproteins/biosynthesis; Polyribosomes/metabolism; Puromycin/pharmacology; Toluene/pharmacology | |||
|- class="tableEdit_footer" | |||
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.170389.U5223990075574&page=170389&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table]</span> || | |||
|} | |} | ||
< | <!--box uid=d41d8cd98f00b204e9800998ecf8427e.170389.U5223990075574--> | ||
==Significance {{HelpIcon|Help:Literature}} == | ==Significance {{HelpIcon|Help:Literature}} == | ||
{{LitSignificance}} | {{LitSignificance}} | ||
| Line 17: | Line 38: | ||
==Annotations {{HelpIcon|Help:Literature}}== | ==Annotations {{HelpIcon|Help:Literature}}== | ||
<protect>< | <protect><annotationlinks/></protect> | ||
==EcoliWiki Links {{HelpIcon|Help:Literature}} == | ==EcoliWiki Links {{HelpIcon|Help:Literature}} == | ||
Latest revision as of 04:53, 15 September 2013
| Citation |
Halegoua, S, Hirashima, A and Inouye, M (1976) Puromycin-resistant biosynthesis of a specific outer-membrane lipoprotein of Escherichia coli. J. Bacteriol. 126:183-91 |
|---|---|
| Abstract |
The reported puromycin resistance of the in vivo biosynthesis of a specific outer-membrane lipoprotein of Escherichia coli was further investigated. The biosynthetic machinery making the lipoprotein was made more accessible to puromycin by disruption of the cell structure using ethylenediaminetetracetate or toluene, and finally in an in vitro protein biosynthesis system using polyribosomes. Puromycin sensitivity of overall protein synthesis increased by about 10-fold for each method of disruption of the cell structure; 50% inhibitions were obtained at 330, 35, 2.7, and 0.22 mug of puromycin per ml for intact cells, ethylenediaminetetraacetate-treated cells, toluene-treated cells, and the polyribosome system, respectively. However, the lipoprotein biosynthesis remained more resistant to puromycin than the biosynthesis of other proteins in all systems tested. These results strongly suggest that puromycin resistance of the lipoprotein biosynthesis is due to an intrinsic property of the lipoprotein biosynthetic machinery. |
| Links | |
| Keywords |
Bacterial Proteins/biosynthesis; Cell Wall/metabolism; Cell-Free System; Edetic Acid/pharmacology; Escherichia coli/drug effects; Escherichia coli/metabolism; Lipoproteins/biosynthesis; Polyribosomes/metabolism; Puromycin/pharmacology; Toluene/pharmacology |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.