WK6mutS(λ): Difference between revisions
Jump to navigation
Jump to search
Created page with "{| class="wikitable" border="1" |- | Name | WK6mutS(λ) |- | Synonyms | |- | Genotype | F' lacI<sup>q</sup> ∆(lacZ)M15 proA<sup>+</sup>B<sup>+</sup> traD36I ∆(lac-proB) g..." |
mNo edit summary |
||
| (2 intermediate revisions by the same user not shown) | |||
| Line 14: | Line 14: | ||
|- | |- | ||
| Sources | | Sources | ||
| [ | | [https://bccm.belspo.be/page/genecorner-hosts-display-razor/388 LMBP 1466] | ||
|- | |- | ||
| References | | References | ||
| Line 23: | Line 23: | ||
Useful strain for site-directed mutagenesis; disabled mismatch repair due to knocked-out mutS.<br /> | Useful strain for site-directed mutagenesis; disabled mismatch repair due to knocked-out mutS.<br /> | ||
The Tn10 insertion renders the strain tetR.<br /> | The Tn10 insertion renders the strain tetR.<br /> | ||
Not to be used as permanent storage host for plasmids because of the danger of accumulating spontaneous mutations.<br / | Not to be used as permanent storage host for plasmids because of the danger of accumulating spontaneous mutations.<br /> | ||
[[Category:BCCM/ | [[Category:BCCM/GeneCorner]][[Category:Strains]] | ||
Latest revision as of 11:33, 28 October 2024
| Name | WK6mutS(λ) |
| Synonyms | |
| Genotype | F' lacIq ∆(lacZ)M15 proA+B+ traD36I ∆(lac-proB) galE rpsL mutS::Tn10 λ |
| Phenotype | Streptomycin resistant, tetracyclin resistant |
| Sources | LMBP 1466 |
| References | PMID:3038536 PMID:2501754 |
Notes
Useful strain for site-directed mutagenesis; disabled mismatch repair due to knocked-out mutS.
The Tn10 insertion renders the strain tetR.
Not to be used as permanent storage host for plasmids because of the danger of accumulating spontaneous mutations.