MG1655 seqA-PAmCherry: Difference between revisions
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Created page with "{| class="wikitable" border="1" |- | Name | MG1655 seqA-PAmCherry |- | Synonyms | |- | Genotype | F<sup>–</sup> λ<sup>–</sup> ilvG<sup>–</sup> rfb-50 rph-1 seqA-PAmChe..." |
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| [ | | [https://bccm.belspo.be/page/genecorner-hosts-display-razor/662 LMBP 9778] | ||
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[[Category:BCCM/ | [[Category:BCCM/GeneCorner]][[Category:Strains]] | ||
Latest revision as of 11:31, 28 October 2024
| Name | MG1655 seqA-PAmCherry |
| Synonyms | |
| Genotype | F– λ– ilvG– rfb-50 rph-1 seqA-PAmCherry:kmR |
| Phenotype | Kanamycin resistant |
| Sources | LMBP 9778 |
| References | PMID:26449690 |
Notes
The native seqA gene was fused at its 3' end to the fluorescent protein gene PAmCherry, separated with a linker (ASPPPGRSR) and followed by a kanamycin resistance marker. The entire cassette was integrated in the chromosome.
PAmCherry is a photoactivatable variant of the red fluorescent protein mCherry. PAmCherry is not fluorescent until it is exposed to 350-400 nm light.
This strain can be used to study the subcellular localization of the E. coli DNA binding protein SeqA.