PMID:5073742: Difference between revisions
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!align=left |Citation | |||
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'''Brockes, JP, Brown, PR and Murray, K''' (1972) The deoxyribonucleic acid modification enzyme of bacteriophage P1. ''Biochem. J.'' '''127''':1-10 | |||
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!align=left |Abstract | |||
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The bacteriophage P1 modification enzyme, assayed by the specific methylation of unmodified bacteriophage 82 DNA, has been purified 500-fold from a bacteriophage P1 lysogen of Escherichia coli. The enzyme catalyses the incorporation of approximately 20-24 methyl groups per bacteriophage 82 DNA molecule. The sole product of methylation is 6-methylaminopurine. Methylation of unmodified bacteriophage DNA confers protection against a challenge by purified bacteriophage P1 restriction enzyme. The pH optimum is 6.0-6.25: the apparent K(m) for S-adenosyl-l-methionine is 5x10(-6)m. | |||
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!align=left |Links | |||
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=5073742 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1178554 PMC1178554] | |||
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!align=left |Keywords | |||
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Ammonium Sulfate; Catalase; Centrifugation, Zonal; Chromatography, DEAE-Cellulose; Coliphages/enzymology; DNA, Viral; Dialysis; Hydrogen-Ion Concentration; Hydrolysis; Methylation; Methyltransferases/isolation & purification; Morpholines; Purines; S-Adenosylmethionine; Sulfonic Acids; Tritium | |||
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==Significance {{HelpIcon|Help:Literature}} == | |||
{{LitSignificance}} | |||
== Useful Materials and Methods {{HelpIcon|Help:Literature}}== | |||
{{LitMaterials}} | |||
==Annotations {{HelpIcon|Help:Literature}}== | |||
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==EcoliWiki Links {{HelpIcon|Help:Literature}} == | |||
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==See also== | |||
==References== | |||
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Latest revision as of 02:05, 7 July 2016
| Citation |
Brockes, JP, Brown, PR and Murray, K (1972) The deoxyribonucleic acid modification enzyme of bacteriophage P1. Biochem. J. 127:1-10 |
|---|---|
| Abstract |
The bacteriophage P1 modification enzyme, assayed by the specific methylation of unmodified bacteriophage 82 DNA, has been purified 500-fold from a bacteriophage P1 lysogen of Escherichia coli. The enzyme catalyses the incorporation of approximately 20-24 methyl groups per bacteriophage 82 DNA molecule. The sole product of methylation is 6-methylaminopurine. Methylation of unmodified bacteriophage DNA confers protection against a challenge by purified bacteriophage P1 restriction enzyme. The pH optimum is 6.0-6.25: the apparent K(m) for S-adenosyl-l-methionine is 5x10(-6)m. |
| Links | |
| Keywords |
Ammonium Sulfate; Catalase; Centrifugation, Zonal; Chromatography, DEAE-Cellulose; Coliphages/enzymology; DNA, Viral; Dialysis; Hydrogen-Ion Concentration; Hydrolysis; Methylation; Methyltransferases/isolation & purification; Morpholines; Purines; S-Adenosylmethionine; Sulfonic Acids; Tritium |
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