PMID:9192629: Difference between revisions

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<span style = 'font-size: large'> '''Bianco, PR and Kowalczykowski, SC'''  (1997) The recombination hotspot Chi is recognized by the translocating RecBCD enzyme as the single strand of DNA containing the sequence 5'-GCTGGTGG-3'.''Proc. Natl. Acad. Sci. U.S.A.'' '''94''':6706-11</span>
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!align=left  |Citation
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'''Bianco, PR and Kowalczykowski, SC'''  (1997) The recombination hotspot Chi is recognized by the translocating RecBCD enzyme as the single strand of DNA containing the sequence 5'-GCTGGTGG-3'. ''Proc. Natl. Acad. Sci. U.S.A.'' '''94''':6706-11
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!align=left  |Abstract
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The RecBCD enzyme of Escherichia coli functions in the seemingly disparate roles of homologous recombination and the degradation of DNA. Which of these two roles it assumes is regulated by the 8-base recombination hotspot, Chi. Using double-stranded DNA substrates that are heteroduplex at the Chi locus we have established the determinants for Chi recognition. Our results show that an actively translocating RecBCD enzyme requires only the sequence information in the 5'-GCTGGTGG-3'-containing strand to recognize and to be regulated by Chi. Furthermore, the RecBCD enzyme can translocate through DNA heteroduplex bubbles as large as 22 bases, and still recognize a Chi sequence embedded in this region. This implies that recognition of Chi occurs following the unwinding of the DNA.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9192629 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC21222 PMC21222]


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|{{TableHeadingColor}} |'''Abstract''' || The RecBCD enzyme of Escherichia coli functions in the seemingly disparate roles of homologous recombination and the degradation of DNA. Which of these two roles it assumes is regulated by the 8-base recombination hotspot, Chi. Using double-stranded DNA substrates that are heteroduplex at the Chi locus we have established the determinants for Chi recognition. Our results show that an actively translocating RecBCD enzyme requires only the sequence information in the 5'-GCTGGTGG-3'-containing strand to recognize and to be regulated by Chi. Furthermore, the RecBCD enzyme can translocate through DNA heteroduplex bubbles as large as 22 bases, and still recognize a Chi sequence embedded in this region. This implies that recognition of Chi occurs following the unwinding of the DNA.
!align=left  |Keywords
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DNA, Recombinant/genetics; DNA, Single-Stranded/genetics; Escherichia coli/genetics; Exodeoxyribonuclease V; Exodeoxyribonucleases/genetics; Recombination, Genetic; Regulatory Sequences, Nucleic Acid/genetics
|{{TableHeadingColor}} nowrap |'''Links''' ||  [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9192629 PubMed]
 
[http://www.ncbi.nlm.nih.gov/entrez/utils/fref.fcgi?PrId=3494&itool=AbstractPlus-nondef&uid=9192629&db=pubmed&url=http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9192629 Full text]
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==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
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Latest revision as of 04:57, 15 September 2013

Citation

Bianco, PR and Kowalczykowski, SC (1997) The recombination hotspot Chi is recognized by the translocating RecBCD enzyme as the single strand of DNA containing the sequence 5'-GCTGGTGG-3'. Proc. Natl. Acad. Sci. U.S.A. 94:6706-11

Abstract

The RecBCD enzyme of Escherichia coli functions in the seemingly disparate roles of homologous recombination and the degradation of DNA. Which of these two roles it assumes is regulated by the 8-base recombination hotspot, Chi. Using double-stranded DNA substrates that are heteroduplex at the Chi locus we have established the determinants for Chi recognition. Our results show that an actively translocating RecBCD enzyme requires only the sequence information in the 5'-GCTGGTGG-3'-containing strand to recognize and to be regulated by Chi. Furthermore, the RecBCD enzyme can translocate through DNA heteroduplex bubbles as large as 22 bases, and still recognize a Chi sequence embedded in this region. This implies that recognition of Chi occurs following the unwinding of the DNA.

Links

PubMed PMC21222

Keywords

DNA, Recombinant/genetics; DNA, Single-Stranded/genetics; Escherichia coli/genetics; Exodeoxyribonuclease V; Exodeoxyribonucleases/genetics; Recombination, Genetic; Regulatory Sequences, Nucleic Acid/genetics

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