PMID:8955422: Difference between revisions

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<span style = 'font-size: large'> '''Callahan, C and Deutscher, MP'''  (1996) Identification and characterization of the Escherichia coli rbn gene encoding the tRNA processing enzyme RNase BN.''J. Bacteriol.'' '''178''':7329-32</span>
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!align=left  |Citation
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'''Callahan, C and Deutscher, MP'''  (1996) Identification and characterization of the Escherichia coli rbn gene encoding the tRNA processing enzyme RNase BN. ''J. Bacteriol.'' '''178''':7329-32
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!align=left  |Abstract
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The gene encoding RNase BN was localized to 88 min on the Escherichia coli chromosome by a novel suppressor assay and conjugational and transductional analysis. Assay of subclones derived from lambda phage 543 of the Kohara library, which encompasses this region of the chromosome, for elevated RNase BN activity identified o290, a previously reported open reading frame, as the gene encoding RNase BN. Interruption of this gene with a Kan(r) cassette and introduction into the chromosome eliminated cellular RNase BN activity but had no effect on cell growth. On the basis of these data, we suggest that o290 be renamed rbn. Potential homologs of rbn in other organisms also were identified.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8955422 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC178653 PMC178653]


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|{{TableHeadingColor}} |'''Abstract''' || The gene encoding RNase BN was localized to 88 min on the Escherichia coli chromosome by a novel suppressor assay and conjugational and transductional analysis. Assay of subclones derived from lambda phage 543 of the Kohara library, which encompasses this region of the chromosome, for elevated RNase BN activity identified o290, a previously reported open reading frame, as the gene encoding RNase BN. Interruption of this gene with a Kan(r) cassette and introduction into the chromosome eliminated cellular RNase BN activity but had no effect on cell growth. On the basis of these data, we suggest that o290 be renamed rbn. Potential homologs of rbn in other organisms also were identified.
!align=left  |Keywords
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Bacterial Proteins/genetics; Chromosome Mapping; Cloning, Molecular; Escherichia coli/enzymology; Exoribonucleases/genetics; Sequence Analysis, DNA
|{{TableHeadingColor}} nowrap |'''Links''' ||  [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8955422 PubMed]
 
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==Annotations {{HelpIcon|Help:Literature}}==
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==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
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Latest revision as of 04:56, 15 September 2013

Citation

Callahan, C and Deutscher, MP (1996) Identification and characterization of the Escherichia coli rbn gene encoding the tRNA processing enzyme RNase BN. J. Bacteriol. 178:7329-32

Abstract

The gene encoding RNase BN was localized to 88 min on the Escherichia coli chromosome by a novel suppressor assay and conjugational and transductional analysis. Assay of subclones derived from lambda phage 543 of the Kohara library, which encompasses this region of the chromosome, for elevated RNase BN activity identified o290, a previously reported open reading frame, as the gene encoding RNase BN. Interruption of this gene with a Kan(r) cassette and introduction into the chromosome eliminated cellular RNase BN activity but had no effect on cell growth. On the basis of these data, we suggest that o290 be renamed rbn. Potential homologs of rbn in other organisms also were identified.

Links

PubMed PMC178653

Keywords

Bacterial Proteins/genetics; Chromosome Mapping; Cloning, Molecular; Escherichia coli/enzymology; Exoribonucleases/genetics; Sequence Analysis, DNA

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