PMID:8655552: Difference between revisions
/Volumes/r3/t3/ul/working/touchTables/touch_all_tables.php |
imported>Wikientrybot change PMID template to TableEdit version |
||
| (2 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
{{RightTOC}} | {{RightTOC}} | ||
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.59507.T52239a691ec4a--> | |||
<!-- | |||
****************************************************************************************** | |||
* | |||
* ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** | |||
* | |||
****************************************************************************************** --> | |||
{| id="T52239a691ec4a" class=" tableEdit PMID_info_table" | |||
|- | |||
!align=left |Citation | |||
|| | |||
'''Francetic, O and Pugsley, AP''' (1996) The cryptic general secretory pathway (gsp) operon of Escherichia coli K-12 encodes functional proteins. ''J. Bacteriol.'' '''178''':3544-9 | |||
|- | |||
!align=left |Abstract | |||
|| | |||
Systematic sequencing of the Escherichia coli K-12 chromosome (GenBank entry U18997) has revealed the presence of an apparently complete operon of genes (the gspC-0 operon) similar to genes coding for components of the main terminal branch of the general secretory pathway (e.g., the Klebsiella oxytoca pulC-0 pullulanase secretion operon) and to related genes required for type IV pilus biogenesis. For example, the last gene in the gsp operon, gspO (formerly hopD), encodes a protein which is similar to several type IV prepilin peptidases. Expression of gspO from lacZp promotes cleavage of two known prepilin peptidase substrates in E. coli K-12: Neisseria gonorrhoeae type IV prepilin and K. oxytoca prePulG protein. gspO also complements a mutation in the corresponding gene (pulO) of the pullulanase secretion operon when it is expressed from lacZp. Another gene in the gsp operon, gspG (formerly hopG), encodes a protein similar to prePulG, a component of the pullulanase secretion pathway. Expression of gspG from lacZp leads to production of a protein which (i) is recognized by PulG-specific antiserum (and by antiserum against the Pseudomonas aeruginosa PulG homolog XcpG [formerly XcpT]), (ii) is processed in cells expressing gspO, and (iii) restores secretion in cells carrying a pulG mutation. The chromosomal copies of gspG and gspO are apparently not expressed, probably because of very weak transcription from the upstream region, as measured by using a chromosomal gspC-lacZ operon fusion. Thus, the gsp operon of E. coli K-12 includes at least two functional genes which, together with the rest of the operon, are probably not expressed under laboratory conditions. | |||
|- | |||
!align=left |Links | |||
|| | |||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8655552 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC178124 PMC178124] | |||
|- | |- | ||
| | !align=left |Keywords | ||
|| | |||
|- | Bacterial Outer Membrane Proteins/metabolism; Bacterial Proteins/genetics; Bacterial Proteins/secretion; Base Sequence; DNA, Bacterial/genetics; Endopeptidases/genetics; Escherichia coli/genetics; Fimbriae Proteins; Gene Expression Regulation, Bacterial; Genes, Bacterial; Genetic Complementation Test; Glycoside Hydrolases/genetics; Molecular Sequence Data; Operon; Promoter Regions, Genetic; RNA, Messenger/genetics; Regulatory Sequences, Nucleic Acid; Transcription, Genetic | ||
|{{ | |||
|- class="tableEdit_footer" | |||
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.59507.T52239a691ec4a&page=59507&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table]</span> || | |||
|} | |} | ||
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.59507.T52239a691ec4a--> | |||
==Significance {{HelpIcon|Help:Literature}} == | ==Significance {{HelpIcon|Help:Literature}} == | ||
{{LitSignificance}} | {{LitSignificance}} | ||
| Line 18: | Line 38: | ||
==Annotations {{HelpIcon|Help:Literature}}== | ==Annotations {{HelpIcon|Help:Literature}}== | ||
< | <annotationlinks/> | ||
==EcoliWiki Links {{HelpIcon|Help:Literature}} == | ==EcoliWiki Links {{HelpIcon|Help:Literature}} == | ||
Latest revision as of 04:55, 15 September 2013
| Citation |
Francetic, O and Pugsley, AP (1996) The cryptic general secretory pathway (gsp) operon of Escherichia coli K-12 encodes functional proteins. J. Bacteriol. 178:3544-9 |
|---|---|
| Abstract |
Systematic sequencing of the Escherichia coli K-12 chromosome (GenBank entry U18997) has revealed the presence of an apparently complete operon of genes (the gspC-0 operon) similar to genes coding for components of the main terminal branch of the general secretory pathway (e.g., the Klebsiella oxytoca pulC-0 pullulanase secretion operon) and to related genes required for type IV pilus biogenesis. For example, the last gene in the gsp operon, gspO (formerly hopD), encodes a protein which is similar to several type IV prepilin peptidases. Expression of gspO from lacZp promotes cleavage of two known prepilin peptidase substrates in E. coli K-12: Neisseria gonorrhoeae type IV prepilin and K. oxytoca prePulG protein. gspO also complements a mutation in the corresponding gene (pulO) of the pullulanase secretion operon when it is expressed from lacZp. Another gene in the gsp operon, gspG (formerly hopG), encodes a protein similar to prePulG, a component of the pullulanase secretion pathway. Expression of gspG from lacZp leads to production of a protein which (i) is recognized by PulG-specific antiserum (and by antiserum against the Pseudomonas aeruginosa PulG homolog XcpG [formerly XcpT]), (ii) is processed in cells expressing gspO, and (iii) restores secretion in cells carrying a pulG mutation. The chromosomal copies of gspG and gspO are apparently not expressed, probably because of very weak transcription from the upstream region, as measured by using a chromosomal gspC-lacZ operon fusion. Thus, the gsp operon of E. coli K-12 includes at least two functional genes which, together with the rest of the operon, are probably not expressed under laboratory conditions. |
| Links | |
| Keywords |
Bacterial Outer Membrane Proteins/metabolism; Bacterial Proteins/genetics; Bacterial Proteins/secretion; Base Sequence; DNA, Bacterial/genetics; Endopeptidases/genetics; Escherichia coli/genetics; Fimbriae Proteins; Gene Expression Regulation, Bacterial; Genes, Bacterial; Genetic Complementation Test; Glycoside Hydrolases/genetics; Molecular Sequence Data; Operon; Promoter Regions, Genetic; RNA, Messenger/genetics; Regulatory Sequences, Nucleic Acid; Transcription, Genetic |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.