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!align=left |Citation | |||
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'''Bischof, O, Kruft, V and Wittmann-Liebold, B''' (1994) Analysis of the puromycin binding site in the 70 S ribosome of Escherichia coli at the peptide level. ''J. Biol. Chem.'' '''269''':18315-9 | |||
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!align=left |Abstract | |||
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Photoinduced cross-linking of Escherichia coli 70 S ribosomes with [3H]puromycin has led to the labeling of ribosomal proteins S7, S14, S18, L18, and L29. Proteolytic fragmentation of these proteins and separation of the peptide mixtures by C18 reversed-phase high performance liquid chromatography resulted in six puromycin-labeled peptides which were applied to sequence analysis. The following peptides were found labeled: Pro1-Lys10 of S7, Ala28-Lys46 and Ala7-Lys11 of S14, Asp24-Lys29 of S18, Tyr64-Lys68 of L18, and Thr55-Lys60 of L29. For the first time the molecular environment of an antibiotic in the procaryotic ribosome is presented at the peptide level. | |||
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8034577 PubMed] | |||
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!align=left |Keywords | |||
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Amino Acid Sequence; Binding Sites; Binding, Competitive; Chromatography, High Pressure Liquid; Escherichia coli/metabolism; Kinetics; Molecular Sequence Data; Peptide Fragments/chemistry; Peptide Fragments/metabolism; Puromycin/metabolism; Ribosomal Proteins/isolation & purification; Ribosomal Proteins/metabolism; Ribosomes/metabolism; Tritium | |||
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==Significance {{HelpIcon|Help:Literature}} == | |||
{{LitSignificance}} | |||
== Useful Materials and Methods {{HelpIcon|Help:Literature}}== | |||
{{LitMaterials}} | |||
==Annotations {{HelpIcon|Help:Literature}}== | |||
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==EcoliWiki Links {{HelpIcon|Help:Literature}} == | |||
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==See also== | |||
==References== | |||
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Latest revision as of 04:52, 15 September 2013
| Citation |
Bischof, O, Kruft, V and Wittmann-Liebold, B (1994) Analysis of the puromycin binding site in the 70 S ribosome of Escherichia coli at the peptide level. J. Biol. Chem. 269:18315-9 |
|---|---|
| Abstract |
Photoinduced cross-linking of Escherichia coli 70 S ribosomes with [3H]puromycin has led to the labeling of ribosomal proteins S7, S14, S18, L18, and L29. Proteolytic fragmentation of these proteins and separation of the peptide mixtures by C18 reversed-phase high performance liquid chromatography resulted in six puromycin-labeled peptides which were applied to sequence analysis. The following peptides were found labeled: Pro1-Lys10 of S7, Ala28-Lys46 and Ala7-Lys11 of S14, Asp24-Lys29 of S18, Tyr64-Lys68 of L18, and Thr55-Lys60 of L29. For the first time the molecular environment of an antibiotic in the procaryotic ribosome is presented at the peptide level. |
| Links | |
| Keywords |
Amino Acid Sequence; Binding Sites; Binding, Competitive; Chromatography, High Pressure Liquid; Escherichia coli/metabolism; Kinetics; Molecular Sequence Data; Peptide Fragments/chemistry; Peptide Fragments/metabolism; Puromycin/metabolism; Ribosomal Proteins/isolation & purification; Ribosomal Proteins/metabolism; Ribosomes/metabolism; Tritium |
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