PMID:7024253: Difference between revisions
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!align=left |Citation | |||
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'''Beher, MG and Schnaitman, CA''' (1981) Regulation of the OmpA outer membrane protein of Escherichia coli. ''J. Bacteriol.'' '''147''':972-85 | |||
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!align=left |Abstract | |||
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The role of lipopolysaccharide in regulating the expression of the ompA outer membrane protein gene of Escherichia coli K-12 was studied by isolating mutants defective in the biosynthesis of lipopolysaccharide and by examining transcription of lacZ in strains carrying operon fusions in which lacZ is expressed from the ompA promoter. By selecting for simultaneous resistance to phages K3 and U3, we obtained mutants defective in rfaC (biosynthesis of core heptose) and in rfaP (phosphorylation of core heptose), and both of these mutant strains failed to express OmpA protein in the outer membrane. Expression of lacZ from the ompA or by foreign ompA alleles which are not expressed in E. coli K-12. Expression was increased in strains carrying rfaC and rfaP mutations. No precursor or degraded form of OmpA protein accumulated in cells which could not express the protein in the outer membrane. This lack of accumulation of precursor was observed even in the presence of phenethyl alcohol, which caused accumulation of OmpA precursor in wild-type cells. We present a model for the regulation of this gene which is consistent with these observations and which involves modulation of transcription coupled to translation of the protein. | |||
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7024253 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC216136 PMC216136] | |||
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| | !align=left |Keywords | ||
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|{{ | Bacterial Outer Membrane Proteins; DNA, Recombinant; Escherichia coli/genetics; Escherichia coli/metabolism; Gene Expression Regulation; Lipopolysaccharides/genetics; Lipopolysaccharides/physiology; Membrane Proteins/genetics; Membrane Proteins/metabolism; Mutation; Operon; Protein Precursors/biosynthesis; Transcription, Genetic | ||
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==Significance {{HelpIcon|Help:Literature}} == | ==Significance {{HelpIcon|Help:Literature}} == | ||
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==EcoliWiki Links {{HelpIcon|Help:Literature}} == | ==EcoliWiki Links {{HelpIcon|Help:Literature}} == | ||
Latest revision as of 04:49, 15 September 2013
| Citation |
Beher, MG and Schnaitman, CA (1981) Regulation of the OmpA outer membrane protein of Escherichia coli. J. Bacteriol. 147:972-85 |
|---|---|
| Abstract |
The role of lipopolysaccharide in regulating the expression of the ompA outer membrane protein gene of Escherichia coli K-12 was studied by isolating mutants defective in the biosynthesis of lipopolysaccharide and by examining transcription of lacZ in strains carrying operon fusions in which lacZ is expressed from the ompA promoter. By selecting for simultaneous resistance to phages K3 and U3, we obtained mutants defective in rfaC (biosynthesis of core heptose) and in rfaP (phosphorylation of core heptose), and both of these mutant strains failed to express OmpA protein in the outer membrane. Expression of lacZ from the ompA or by foreign ompA alleles which are not expressed in E. coli K-12. Expression was increased in strains carrying rfaC and rfaP mutations. No precursor or degraded form of OmpA protein accumulated in cells which could not express the protein in the outer membrane. This lack of accumulation of precursor was observed even in the presence of phenethyl alcohol, which caused accumulation of OmpA precursor in wild-type cells. We present a model for the regulation of this gene which is consistent with these observations and which involves modulation of transcription coupled to translation of the protein. |
| Links | |
| Keywords |
Bacterial Outer Membrane Proteins; DNA, Recombinant; Escherichia coli/genetics; Escherichia coli/metabolism; Gene Expression Regulation; Lipopolysaccharides/genetics; Lipopolysaccharides/physiology; Membrane Proteins/genetics; Membrane Proteins/metabolism; Mutation; Operon; Protein Precursors/biosynthesis; Transcription, Genetic |
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