PMID:6369246: Difference between revisions

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<span style = 'font-size: large'> '''Bencini, DA, Houghton, JE, Hoover, TA, Foltermann, KF, Wild, JR and O'Donovan, GA'''  (1983) The DNA sequence of argI from Escherichia coli K12.''Nucleic Acids Res.'' '''11''':8509-18</span>
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!align=left  |Citation
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'''Bencini, DA, Houghton, JE, Hoover, TA, Foltermann, KF, Wild, JR and O'Donovan, GA'''  (1983) The DNA sequence of argI from Escherichia coli K12. ''Nucleic Acids Res.'' '''11''':8509-18
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!align=left  |Abstract
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The argI gene from E. coli K12 has been sequenced. It contains an open reading frame of 1002 bases which encodes a polypeptide of 334 amino acids. Three such polypeptides are required to form the functional catalytic trimer (c3) of ornithine transcarbamoylase (OTCase-1, EC 2.1.3.3). The molecular mass of the mature trimer deduced from the amino acid sequence is 114,465 daltons. An altered form of argI was produced when a 1.6 kilobase DdeI fragment was subcloned into the HincII site of plasmid pUC8 extending the open reading frame an additional 20 nucleotides. It has been previously reported that the amino-terminal region of the respective polypeptides of argI, argF, and pyrB of E. coli possessed significant homology. In contrast, the homologous promoter/operator regions of argI and argF did not appear to share any homologies with pyrB. However, a closer scrutiny of the nucleotide sequence immediately preceding the pyrBI attenuator revealed a remarkable similarity to the argI and argF control region.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6369246 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC326599 PMC326599]


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|{{TableHeadingColor}} |'''Abstract''' || The argI gene from E. coli K12 has been sequenced. It contains an open reading frame of 1002 bases which encodes a polypeptide of 334 amino acids. Three such polypeptides are required to form the functional catalytic trimer (c3) of ornithine transcarbamoylase (OTCase-1, EC 2.1.3.3). The molecular mass of the mature trimer deduced from the amino acid sequence is 114,465 daltons. An altered form of argI was produced when a 1.6 kilobase DdeI fragment was subcloned into the HincII site of plasmid pUC8 extending the open reading frame an additional 20 nucleotides. It has been previously reported that the amino-terminal region of the respective polypeptides of argI, argF, and pyrB of E. coli possessed significant homology. In contrast, the homologous promoter/operator regions of argI and argF did not appear to share any homologies with pyrB. However, a closer scrutiny of the nucleotide sequence immediately preceding the pyrBI attenuator revealed a remarkable similarity to the argI and argF control region.
!align=left  |Keywords
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Amino Acid Sequence; Base Sequence; Cloning, Molecular; DNA, Bacterial/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Genes; Genes, Bacterial; Ornithine Carbamoyltransferase/genetics; Plasmids
|{{TableHeadingColor}} nowrap |'''Links''' ||  [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6369246 PubMed]
 
[http://www.ncbi.nlm.nih.gov/entrez/utils/fref.fcgi?PrId=3494&itool=AbstractPlus-nondef&uid=6369246&db=pubmed&url=http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=6369246 Full text]
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Latest revision as of 04:49, 15 September 2013

Citation

Bencini, DA, Houghton, JE, Hoover, TA, Foltermann, KF, Wild, JR and O'Donovan, GA (1983) The DNA sequence of argI from Escherichia coli K12. Nucleic Acids Res. 11:8509-18

Abstract

The argI gene from E. coli K12 has been sequenced. It contains an open reading frame of 1002 bases which encodes a polypeptide of 334 amino acids. Three such polypeptides are required to form the functional catalytic trimer (c3) of ornithine transcarbamoylase (OTCase-1, EC 2.1.3.3). The molecular mass of the mature trimer deduced from the amino acid sequence is 114,465 daltons. An altered form of argI was produced when a 1.6 kilobase DdeI fragment was subcloned into the HincII site of plasmid pUC8 extending the open reading frame an additional 20 nucleotides. It has been previously reported that the amino-terminal region of the respective polypeptides of argI, argF, and pyrB of E. coli possessed significant homology. In contrast, the homologous promoter/operator regions of argI and argF did not appear to share any homologies with pyrB. However, a closer scrutiny of the nucleotide sequence immediately preceding the pyrBI attenuator revealed a remarkable similarity to the argI and argF control region.

Links

PubMed PMC326599

Keywords

Amino Acid Sequence; Base Sequence; Cloning, Molecular; DNA, Bacterial/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Genes; Genes, Bacterial; Ornithine Carbamoyltransferase/genetics; Plasmids

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