PMID:3888224: Difference between revisions

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<span style = 'font-size: large'> '''Kita, K, Murakami, H, Oya, H and Anraku, Y'''  (1985) Quantitative determination of cytochromes in the aerobic respiratory chain of Escherichia coli by high-performance liquid chromatography and its application to analysis of mitochondrial cytochromes.''Biochem. Int.'' '''10''':319-26</span>
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'''Kita, K, Murakami, H, Oya, H and Anraku, Y'''  (1985) Quantitative determination of cytochromes in the aerobic respiratory chain of Escherichia coli by high-performance liquid chromatography and its application to analysis of mitochondrial cytochromes. ''Biochem. Int.'' '''10''':319-26
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!align=left  |Abstract
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A method was established for quantitative determination of the composition and the amounts of cytochromes in the aerobic respiratory chain of Escherichia coli. Cytochromes were solubilized with 3%(W/V) Sarkosyl and the extract was analyzed by high-performance liquid chromatography on a gel permeation column of TSK gel-G3000SW. This analytical system required only about 0.5 mg of membrane protein and 20 min per assay. The effects of the gene dosage of F-prime factors and plasmids on cytochromes were analyzed with this system, and the system was applied to the analysis of mitochondrial cytochromes.
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!align=left  |Links
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3888224 PubMed]


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|{{TableHeadingColor}} |'''Abstract''' || A method was established for quantitative determination of the composition and the amounts of cytochromes in the aerobic respiratory chain of Escherichia coli. Cytochromes were solubilized with 3%(W/V) Sarkosyl and the extract was analyzed by high-performance liquid chromatography on a gel permeation column of TSK gel-G3000SW. This analytical system required only about 0.5 mg of membrane protein and 20 min per assay. The effects of the gene dosage of F-prime factors and plasmids on cytochromes were analyzed with this system, and the system was applied to the analysis of mitochondrial cytochromes.
!align=left  |Keywords
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Aerobiosis; Animals; Chromatography, High Pressure Liquid; Cytochrome b Group/metabolism; Cytochromes/isolation & purification; Cytochromes/metabolism; Escherichia coli/metabolism; Mitochondria, Liver/metabolism; Oxygen Consumption; Rats; Species Specificity
|{{TableHeadingColor}} nowrap |'''Links''' ||  [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3888224 PubMed]
 
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Latest revision as of 04:47, 15 September 2013

Citation

Kita, K, Murakami, H, Oya, H and Anraku, Y (1985) Quantitative determination of cytochromes in the aerobic respiratory chain of Escherichia coli by high-performance liquid chromatography and its application to analysis of mitochondrial cytochromes. Biochem. Int. 10:319-26

Abstract

A method was established for quantitative determination of the composition and the amounts of cytochromes in the aerobic respiratory chain of Escherichia coli. Cytochromes were solubilized with 3%(W/V) Sarkosyl and the extract was analyzed by high-performance liquid chromatography on a gel permeation column of TSK gel-G3000SW. This analytical system required only about 0.5 mg of membrane protein and 20 min per assay. The effects of the gene dosage of F-prime factors and plasmids on cytochromes were analyzed with this system, and the system was applied to the analysis of mitochondrial cytochromes.

Links

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Keywords

Aerobiosis; Animals; Chromatography, High Pressure Liquid; Cytochrome b Group/metabolism; Cytochromes/isolation & purification; Cytochromes/metabolism; Escherichia coli/metabolism; Mitochondria, Liver/metabolism; Oxygen Consumption; Rats; Species Specificity

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