PMID:3015603: Difference between revisions
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!align=left |Citation | |||
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'''Besse, M, von Wilcken-Bergmann, B and Müller-Hill, B''' (1986) Synthetic lac operator mediates repression through lac repressor when introduced upstream and downstream from lac promoter. ''EMBO J.'' '''5''':1377-81 | |||
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!align=left |Abstract | |||
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Plasmids were constructed which carry a synthetic lac operator at various distances from the lac promoter. They were tested in vivo for function in the presence and absence of lac repressor. We found significant repression when the lac operator is situated at the 3' end of the lac I gene or at the 5' end of the lac Z gene. When lac operators are inserted at both sites, we found a greater than 150-fold repression. The complex between lac repressor and DNA carrying these two lac operators is exceedingly stable in vitro suggesting that one tetrameric lac repressor may bind to both lac operators. | |||
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!align=left |Links | |||
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3015603 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1166951 PMC1166951] | |||
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| | !align=left |Keywords | ||
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|- | Base Sequence; DNA Restriction Enzymes; Escherichia coli/enzymology; Escherichia coli/genetics; Genes; Genes, Bacterial; Lac Operon; Plasmids; Promoter Regions, Genetic; Repressor Proteins/genetics; Transcription Factors/genetics; beta-Galactosidase/genetics | ||
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==Significance {{HelpIcon|Help:Literature}} == | ==Significance {{HelpIcon|Help:Literature}} == | ||
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Latest revision as of 04:45, 15 September 2013
| Citation |
Besse, M, von Wilcken-Bergmann, B and Müller-Hill, B (1986) Synthetic lac operator mediates repression through lac repressor when introduced upstream and downstream from lac promoter. EMBO J. 5:1377-81 |
|---|---|
| Abstract |
Plasmids were constructed which carry a synthetic lac operator at various distances from the lac promoter. They were tested in vivo for function in the presence and absence of lac repressor. We found significant repression when the lac operator is situated at the 3' end of the lac I gene or at the 5' end of the lac Z gene. When lac operators are inserted at both sites, we found a greater than 150-fold repression. The complex between lac repressor and DNA carrying these two lac operators is exceedingly stable in vitro suggesting that one tetrameric lac repressor may bind to both lac operators. |
| Links | |
| Keywords |
Base Sequence; DNA Restriction Enzymes; Escherichia coli/enzymology; Escherichia coli/genetics; Genes; Genes, Bacterial; Lac Operon; Plasmids; Promoter Regions, Genetic; Repressor Proteins/genetics; Transcription Factors/genetics; beta-Galactosidase/genetics |
| edit table |
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