PMID:2681148: Difference between revisions
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!align=left |Citation | |||
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'''Michelsen, U, Bösl, M, Dingermann, T and Kersten, H''' (1989) The tyrT locus of Escherichia coli exhibits a regulatory function for glycine metabolism. ''J. Bacteriol.'' '''171''':5987-94 | |||
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!align=left |Abstract | |||
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The tyrT locus in Escherichia coli codes for two gene copies of tRNA(1Tyr). Both genes are organized in one operon, which has a unique structure. The two tRNA genes are separated by a spacer segment highly homologous to a part of a unit which is repeated three times in the distal portion of the locus. This operon also contains coding capacity for a small basic protein. A genomic deletion of this locus was constructed and marked by a kanamycin resistance cassette. Deletion mutants exhibited a characteristic phenotype when cells were shifted from rich medium to minimal medium. The cells entered a transient lag phase, apparently resulting from specific glycine starvation. This phenotype involved stringent response and was therefore not observed in relA derivatives. The genomic deletion was complemented in trans by a plasmid-borne tyrT locus. From deletion mapping, it can be concluded that a product of the tyrT operon is responsible for complementation. However, neither the tRNA(1Tyr) nor the proposed basic protein is the complementation-competent entity. | |||
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!align=left |Links | |||
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2681148 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC210463 PMC210463] | |||
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| | !align=left |Keywords | ||
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|- | Amino Acids/metabolism; Blotting, Southern; Chromosome Deletion; Cloning, Molecular; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/metabolism; Genes, Bacterial; Genes, Regulator; Genetic Complementation Test; Genotype; Glycine/metabolism; Mutation; Nucleic Acid Hybridization; RNA, Transfer, Amino Acid-Specific/genetics; RNA, Transfer, Tyr/genetics; Restriction Mapping | ||
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==Significance {{HelpIcon|Help:Literature}} == | ==Significance {{HelpIcon|Help:Literature}} == | ||
{{LitSignificance}} | {{LitSignificance}} | ||
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==Annotations {{HelpIcon|Help:Literature}}== | ==Annotations {{HelpIcon|Help:Literature}}== | ||
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==EcoliWiki Links {{HelpIcon|Help:Literature}} == | ==EcoliWiki Links {{HelpIcon|Help:Literature}} == | ||
Latest revision as of 04:44, 15 September 2013
| Citation |
Michelsen, U, Bösl, M, Dingermann, T and Kersten, H (1989) The tyrT locus of Escherichia coli exhibits a regulatory function for glycine metabolism. J. Bacteriol. 171:5987-94 |
|---|---|
| Abstract |
The tyrT locus in Escherichia coli codes for two gene copies of tRNA(1Tyr). Both genes are organized in one operon, which has a unique structure. The two tRNA genes are separated by a spacer segment highly homologous to a part of a unit which is repeated three times in the distal portion of the locus. This operon also contains coding capacity for a small basic protein. A genomic deletion of this locus was constructed and marked by a kanamycin resistance cassette. Deletion mutants exhibited a characteristic phenotype when cells were shifted from rich medium to minimal medium. The cells entered a transient lag phase, apparently resulting from specific glycine starvation. This phenotype involved stringent response and was therefore not observed in relA derivatives. The genomic deletion was complemented in trans by a plasmid-borne tyrT locus. From deletion mapping, it can be concluded that a product of the tyrT operon is responsible for complementation. However, neither the tRNA(1Tyr) nor the proposed basic protein is the complementation-competent entity. |
| Links | |
| Keywords |
Amino Acids/metabolism; Blotting, Southern; Chromosome Deletion; Cloning, Molecular; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/metabolism; Genes, Bacterial; Genes, Regulator; Genetic Complementation Test; Genotype; Glycine/metabolism; Mutation; Nucleic Acid Hybridization; RNA, Transfer, Amino Acid-Specific/genetics; RNA, Transfer, Tyr/genetics; Restriction Mapping |
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