PMID:10617639: Difference between revisions
/Volumes/r3/t3/ul/working/touchTables/touch_all_tables.php |
imported>Wikientrybot change PMID template to TableEdit version |
||
| (2 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
{{RightTOC}} | {{RightTOC}} | ||
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.59488.H52237e504eca5--> | |||
<!-- | |||
****************************************************************************************** | |||
* | |||
* ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** | |||
* | |||
****************************************************************************************** --> | |||
{| id="H52237e504eca5" class=" tableEdit PMID_info_table" | |||
|- | |||
!align=left |Citation | |||
|| | |||
'''Hindson, VJ, Moody, PC, Rowe, AJ and Shaw, WV''' (2000) Serine acetyltransferase from Escherichia coli is a dimer of trimers. ''J. Biol. Chem.'' '''275''':461-6 | |||
|- | |||
!align=left |Abstract | |||
|| | |||
Equilibrium sedimentation studies show that the serine acetyltransferase (SAT) of Escherichia coli is a hexamer. The results of velocity sedimentation and quasi-elastic light scattering experiments suggest that the identical subunits are loosely packed and/or arranged in an ellipsoidal fashion. Chemical cross-linking studies indicate that the fundamental unit of quaternary structure is a trimer. The likelihood, therefore, is that in solution SAT exists as an open arrangement of paired trimers. Crystals of SAT have 32 symmetry, consistent with such an arrangement, and the cell density function is that expected for a hexamer. Electron microscopy with negative staining provides further evidence that SAT has an ellipsoidal subunit organization, the dimensions of the particles consistent with the proposed paired trimeric subunit arrangement. A bead model analysis supports the view that SAT has a low packing density and, furthermore, indicates that the monomers may have an ellipsoidal shape. Such a view is in keeping with the ellipsoidal subunit shape of trimeric LpxA, an acyltransferase with which SAT shares contiguous repeats of a hexapeptide motif. | |||
|- | |||
!align=left |Links | |||
|| | |||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10617639 PubMed] | |||
|- | |- | ||
| | !align=left |Keywords | ||
|| | |||
|- | Acetyltransferases/chemistry; Acetyltransferases/ultrastructure; Chromatography, Gel; Cross-Linking Reagents; Crystallography; Diffusion; Dimerization; Escherichia coli/enzymology; Microscopy, Electron; Protein Structure, Quaternary; Serine O-Acetyltransferase; Ultracentrifugation | ||
|{{ | |||
|- class="tableEdit_footer" | |||
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.59488.H52237e504eca5&page=59488&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table]</span> || | |||
|} | |} | ||
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.59488.H52237e504eca5--> | |||
==Significance {{HelpIcon|Help:Literature}} == | ==Significance {{HelpIcon|Help:Literature}} == | ||
{{LitSignificance}} | {{LitSignificance}} | ||
| Line 18: | Line 38: | ||
==Annotations {{HelpIcon|Help:Literature}}== | ==Annotations {{HelpIcon|Help:Literature}}== | ||
< | <annotationlinks/> | ||
==EcoliWiki Links {{HelpIcon|Help:Literature}} == | ==EcoliWiki Links {{HelpIcon|Help:Literature}} == | ||
Latest revision as of 04:11, 15 September 2013
| Citation |
Hindson, VJ, Moody, PC, Rowe, AJ and Shaw, WV (2000) Serine acetyltransferase from Escherichia coli is a dimer of trimers. J. Biol. Chem. 275:461-6 |
|---|---|
| Abstract |
Equilibrium sedimentation studies show that the serine acetyltransferase (SAT) of Escherichia coli is a hexamer. The results of velocity sedimentation and quasi-elastic light scattering experiments suggest that the identical subunits are loosely packed and/or arranged in an ellipsoidal fashion. Chemical cross-linking studies indicate that the fundamental unit of quaternary structure is a trimer. The likelihood, therefore, is that in solution SAT exists as an open arrangement of paired trimers. Crystals of SAT have 32 symmetry, consistent with such an arrangement, and the cell density function is that expected for a hexamer. Electron microscopy with negative staining provides further evidence that SAT has an ellipsoidal subunit organization, the dimensions of the particles consistent with the proposed paired trimeric subunit arrangement. A bead model analysis supports the view that SAT has a low packing density and, furthermore, indicates that the monomers may have an ellipsoidal shape. Such a view is in keeping with the ellipsoidal subunit shape of trimeric LpxA, an acyltransferase with which SAT shares contiguous repeats of a hexapeptide motif. |
| Links | |
| Keywords |
Acetyltransferases/chemistry; Acetyltransferases/ultrastructure; Chromatography, Gel; Cross-Linking Reagents; Crystallography; Diffusion; Dimerization; Escherichia coli/enzymology; Microscopy, Electron; Protein Structure, Quaternary; Serine O-Acetyltransferase; Ultracentrifugation |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.