pSP64: Difference between revisions
Jump to navigation
Jump to search
edited by Miriam via TableEdit |
|||
| (12 intermediate revisions by 5 users not shown) | |||
| Line 1: | Line 1: | ||
==At a Glance == | ==At a Glance == | ||
[[Image: | |||
[[Image:PSP64_GCK.jpg|thumb|450px|right|pSP64, Key: Red = Ampicillin marker, Magenta = SP6 promoter, Stripes = MCS]] | |||
<protect><!--box uid=63ce1649aab8b3180235b0af7618f667.0.O471c1ab2cfb28--> | <protect><!--box uid=63ce1649aab8b3180235b0af7618f667.0.O471c1ab2cfb28--> | ||
<!-- | <!-- | ||
****************************************************************************************** | ****************************************************************************************** | ||
* | * | ||
* ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** | * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** | ||
* | * | ||
****************************************************************************************** --> | ****************************************************************************************** --> | ||
{|{{Prettytable}} width='30%' align=left | {| {{Prettytable}} width='30%' align=left id="O471c1ab2cfb28" class=" tableEdit PlasmidTable" | ||
|- | |- | ||
!{{table heading style}} |Type | !align=left {{table heading style}} |Type | ||
| | || | ||
Cloning | Cloning | ||
|- | |- | ||
!{{table heading style}} |Origin of Replication | !align=left {{table heading style}} |Origin of Replication | ||
| | || | ||
|- | |- | ||
!{{table heading style}} |Copy # | !align=left {{table heading style}} |Copy # | ||
| | || | ||
|- | |- | ||
!{{table heading style}} |Markers | !align=left {{table heading style}} |Markers | ||
| | || | ||
Ampicillin | Ampicillin | ||
|- | |- | ||
!{{table heading style}} |Link to Sequence | !align=left {{table heading style}} |Link to Sequence | ||
| | || | ||
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=58229 PubMed] | [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=58229 PubMed] | ||
|- | |- | ||
!{{table heading style}} |Features | !align=left {{table heading style}} |Features | ||
| | || | ||
SP6 promoter<br> | SP6 promoter<br> | ||
multiple cloning sites<br> | multiple cloning sites<br> | ||
| Line 40: | Line 42: | ||
Hind III, Pst I, Sal I, Acc I, Hinc II,Xba I, Ava I, BamH I, Sma I, Sac I, EcoR I | Hind III, Pst I, Sal I, Acc I, Hinc II,Xba I, Ava I, BamH I, Sma I, Sac I, EcoR I | ||
|- | |- | ||
!{{table heading style}} |Accession Number | !align=left {{table heading style}} |Accession Number | ||
| | || | ||
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=X65327 X65327] | |||
|- | |||
| | |- class="tableEdit_footer" | ||
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.O471c1ab2cfb28&page=0&pagename={{FULLPAGENAMEE}}&type=1&template=PlasmidTable edit table]</span> || | |||
|} | |} | ||
<!--box uid=63ce1649aab8b3180235b0af7618f667.0.O471c1ab2cfb28--></protect> | <!--box uid=63ce1649aab8b3180235b0af7618f667.0.O471c1ab2cfb28--></protect> | ||
<BR> | <BR> | ||
<Br clear='all'> | <Br clear='all'> | ||
==Notes== | ==Notes== | ||
Used for cloning, in vitro transcription. | |||
==History {{HelpIcon|Help:Plasmid}}== | ==History {{HelpIcon|Help:Plasmid}}== | ||
| Line 55: | Line 60: | ||
==Sources == | ==Sources == | ||
[http://www.promega.com/vectors/ | [http://www.promega.com/products/pm/discontinued-vectors/#DV14 Promega]. Discontinued. | ||
==References== | ==References== | ||
{{BacktoTop}} | |||
{{RefHelp}} | {{RefHelp}} | ||
<references/> | <references /> | ||
==Sequence == | ==Sequence == | ||
Latest revision as of 18:30, 22 August 2011
At a Glance
| Type |
Cloning |
|---|---|
| Origin of Replication | |
| Copy # | |
| Markers |
Ampicillin |
| Link to Sequence | |
| Features |
SP6 promoter Hind III, Pst I, Sal I, Acc I, Hinc II,Xba I, Ava I, BamH I, Sma I, Sac I, EcoR I |
| Accession Number | |
| edit table |
Notes
Used for cloning, in vitro transcription.
History
You can help EcoliWiki by writing about the history of this plasmid.
Sources
Promega. Discontinued.
References
See Help:References for how to manage references in EcoliWiki.
Sequence
1 gaatacaagc ttgggctgca ggtcgactct agaggatccc cgggcgagct cgaattcgta
61 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
121 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
181 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
241 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
301 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
361 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
421 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
481 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
541 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
601 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
661 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
721 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
781 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
841 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
901 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
961 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
1021 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
1081 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
1141 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
1201 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
1261 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
1321 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
1381 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
1441 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
1501 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
1561 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
1621 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
1681 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
1741 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
1801 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
1861 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
1921 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
1981 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
2041 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
2101 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
2161 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
2221 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
2281 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
2341 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
2401 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
2461 gagagtgcac catatcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt
2521 agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg
2581 cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg
2641 agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca
2701 accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatctggcta
2761 gcgatgaccc tgctgattgg ttcgctgacc atttccgggg tgcggaacgg cgttaccaga
2821 aactcagaag gttcgtccaa ccaaaccgac tctgacggca gtttacgaga gagatgatag
2881 ggtctgcttc agtaagccag atgctacaca attaggcttg tacatattgt cgttagaacg
2941 cggctacaat taatacataa ccttatgtat catacacata cgatttaggt gacactata