pSP64: Difference between revisions

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New page: ==At a Glance {{HelpIcon|Help:Plasmid}}== <protect><!--box uid=63ce1649aab8b3180235b0af7618f667.0.O471c1ab2cfb28--> <!-- *******************************************************************...
 
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==At a Glance {{HelpIcon|Help:Plasmid}}==
==At a Glance ==
 
[[Image:PSP64_GCK.jpg|thumb|450px|right|pSP64, Key: Red = Ampicillin marker, Magenta = SP6 promoter, Stripes = MCS]]
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{|{{Prettytable}} width='30%' align=left
{| {{Prettytable}} width='30%' align=left id="O471c1ab2cfb28"  class=" tableEdit PlasmidTable" 
 
|-
|-
!{{table heading style}} |Type
!align=left {{table heading style}} |Type
||
Cloning
|-
|-
!{{table heading style}} |Origin of Replication
!align=left {{table heading style}} |Origin of Replication
||
 
|-
|-
!{{table heading style}} |Copy #
!align=left {{table heading style}} |Copy #
||
 
|-
|-
!{{table heading style}} |Markers
!align=left {{table heading style}} |Markers
||
Ampicillin
|-
|-
!{{table heading style}} |Link to Sequence
!align=left {{table heading style}} |Link to Sequence
||
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=58229 PubMed]
|-
|-
!{{table heading style}} |Comments
!align=left {{table heading style}} |Features
||
SP6 promoter<br>                   
multiple cloning sites<br>       
lacZ start codon<br>                       
lac operon<br>               
lac operator<br>                   
 
Hind III, Pst  I, Sal  I, Acc  I, Hinc II,Xba  I, Ava  I, BamH I, Sma  I, Sac  I, EcoR I
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!align=left {{table heading style}} |Accession Number
||
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=X65327 X65327]
 
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==Notes==
Used for cloning, in vitro transcription.
==History {{HelpIcon|Help:Plasmid}}==
==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}
{{PlasmidHistory}}


==Sources {{HelpIcon|Help:Plasmid}}==
==Sources ==
{{PlasmidSources}}
[http://www.promega.com/products/pm/discontinued-vectors/#DV14 Promega]. Discontinued.


==References==
==References==
{{BacktoTop}}
{{RefHelp}}
{{RefHelp}}
<references/>
<references />
 
==Sequence {{HelpIcon|Help:Plasmid}}==
{{PlasmidSeq}}


==Sequence ==
        1 gaatacaagc ttgggctgca ggtcgactct agaggatccc cgggcgagct cgaattcgta
      61 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
      121 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
      181 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
      241 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
      301 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
      361 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
      421 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
      481 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
      541 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
      601 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
      661 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
      721 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
      781 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
      841 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
      901 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
      961 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    1021 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    1081 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
    1141 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
    1201 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
    1261 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
    1321 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
    1381 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
    1441 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    1501 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    1561 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    1621 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    1681 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    1741 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    1801 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    1861 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    1921 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    1981 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    2041 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    2101 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    2161 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    2221 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
    2281 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
    2341 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
    2401 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
    2461 gagagtgcac catatcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt
    2521 agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg
    2581 cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg
    2641 agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca
    2701 accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatctggcta
    2761 gcgatgaccc tgctgattgg ttcgctgacc atttccgggg tgcggaacgg cgttaccaga
    2821 aactcagaag gttcgtccaa ccaaaccgac tctgacggca gtttacgaga gagatgatag
    2881 ggtctgcttc agtaagccag atgctacaca attaggcttg tacatattgt cgttagaacg
    2941 cggctacaat taatacataa ccttatgtat catacacata cgatttaggt gacactata






[[Category:Plasmids]]
[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]][[Category:SP6 Promoter]]
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Latest revision as of 18:30, 22 August 2011

At a Glance

pSP64, Key: Red = Ampicillin marker, Magenta = SP6 promoter, Stripes = MCS
Type

Cloning

Origin of Replication
Copy #
Markers

Ampicillin

Link to Sequence

PubMed

Features

SP6 promoter
multiple cloning sites
lacZ start codon
lac operon
lac operator

Hind III, Pst I, Sal I, Acc I, Hinc II,Xba I, Ava I, BamH I, Sma I, Sac I, EcoR I

Accession Number

X65327

edit table



Notes

Used for cloning, in vitro transcription.

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Promega. Discontinued.

References

[back to top]


See Help:References for how to manage references in EcoliWiki.


Sequence

       1 gaatacaagc ttgggctgca ggtcgactct agaggatccc cgggcgagct cgaattcgta
      61 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     121 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     181 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     241 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     301 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     361 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     421 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     481 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     541 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     601 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     661 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     721 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     781 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     841 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     901 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     961 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    1021 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    1081 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
    1141 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
    1201 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
    1261 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
    1321 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
    1381 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
    1441 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    1501 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    1561 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    1621 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    1681 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    1741 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    1801 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    1861 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    1921 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    1981 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    2041 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    2101 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    2161 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    2221 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
    2281 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
    2341 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
    2401 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
    2461 gagagtgcac catatcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt
    2521 agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg
    2581 cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg
    2641 agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca
    2701 accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatctggcta
    2761 gcgatgaccc tgctgattgg ttcgctgacc atttccgggg tgcggaacgg cgttaccaga
    2821 aactcagaag gttcgtccaa ccaaaccgac tctgacggca gtttacgaga gagatgatag
    2881 ggtctgcttc agtaagccag atgctacaca attaggcttg tacatattgt cgttagaacg
    2941 cggctacaat taatacataa ccttatgtat catacacata cgatttaggt gacactata
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